Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607 |
Resumo: | Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (Isc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked Isc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum Isc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression. |
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Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine modelUndernutritionIon transportIntestinal absorptionUndernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (Isc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked Isc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum Isc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression.Associação Brasileira de Divulgação Científica2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607Brazilian Journal of Medical and Biological Research v.49 n.10 2016reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20165340info:eu-repo/semantics/openAccessSampaio,I.C.Medeiros,P.H.Q.S.Rodrigues,F.A.P.Cavalcante,P.A.Ribeiro,S.A.Oliveira,J.S.Prata,M.M.G.Costa,D.V.S.Fonseca,S.G.C.Guedes,M.M.Soares,A.M.Brito,G.A.C.Havt,A.Moore,S.R.Lima,A.A.M.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2016001000607Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
title |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
spellingShingle |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model Sampaio,I.C. Undernutrition Ion transport Intestinal absorption |
title_short |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
title_full |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
title_fullStr |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
title_full_unstemmed |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
title_sort |
Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model |
author |
Sampaio,I.C. |
author_facet |
Sampaio,I.C. Medeiros,P.H.Q.S. Rodrigues,F.A.P. Cavalcante,P.A. Ribeiro,S.A. Oliveira,J.S. Prata,M.M.G. Costa,D.V.S. Fonseca,S.G.C. Guedes,M.M. Soares,A.M. Brito,G.A.C. Havt,A. Moore,S.R. Lima,A.A.M. |
author_role |
author |
author2 |
Medeiros,P.H.Q.S. Rodrigues,F.A.P. Cavalcante,P.A. Ribeiro,S.A. Oliveira,J.S. Prata,M.M.G. Costa,D.V.S. Fonseca,S.G.C. Guedes,M.M. Soares,A.M. Brito,G.A.C. Havt,A. Moore,S.R. Lima,A.A.M. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sampaio,I.C. Medeiros,P.H.Q.S. Rodrigues,F.A.P. Cavalcante,P.A. Ribeiro,S.A. Oliveira,J.S. Prata,M.M.G. Costa,D.V.S. Fonseca,S.G.C. Guedes,M.M. Soares,A.M. Brito,G.A.C. Havt,A. Moore,S.R. Lima,A.A.M. |
dc.subject.por.fl_str_mv |
Undernutrition Ion transport Intestinal absorption |
topic |
Undernutrition Ion transport Intestinal absorption |
description |
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (Isc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked Isc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum Isc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000607 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20165340 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.49 n.10 2016 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302945361395712 |