Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats

Detalhes bibliográficos
Autor(a) principal: de-Oliveira,G.R.
Data de Publicação: 1998
Outros Autores: Gondim,F. de-A.A., da-Graça,J.R.V., Xavier-Neto,J., Dantas,R.P., Gondim,R.B.M., Santos,A.A., Rola,F.H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017
Resumo: The present study evaluates the effect of blood volume expansion on the gastrointestinal transit of a charchoal meal (2.5 ml of an aqueous suspension consisting of 5% charcoal and 5% gum arabic) in awake male Wistar rats (200-270 g). On the day before the experiments, the rats were anesthetized with ether, submitted to left jugular vein cannulation and fasted with water ad libitum until 2 h before the gastrointestinal transit measurement. Blood volume expansion by iv infusion of 1 ml/min Ringer bicarbonate in volumes of 3, 4 or 5% body weight delayed gastrointestinal transit at 10 min after test meal administration by 21.3-26.7% (P<0.05), but no effect was observed after 1 or 2% body weight expansion. The effect of blood volume expansion (up to 5% body weight) on gastrointestinal transit lasted for at least 60 min (P<0.05). Mean arterial pressure increased transiently and central venous pressure increased and hematocrit decreased (P<0.05). Subdiaphragmatic vagotomy and yohimbine (3 mg/kg) prevented the delay caused by expansion on gastrointestinal transit, while atropine (0.5 mg/kg), L-NAME (2 mg/kg), hexamethonium (10 mg/kg), prazosin (1 mg/kg) or propranolol (2 mg/kg) were ineffective. These data show that blood volume expansion delays the gastrointestinal transit of a charcoal meal and that vagal and yohimbine-sensitive pathways appear to be involved in this phenomenon. The delay in gastrointestinal transit observed here, taken together with the modifications of gastrointestinal permeability to salt and water reported by others, may be part of the mechanisms involved in liquid excess management.
id ABDC-1_e8f5b09174a2e3890158190893ea9284
oai_identifier_str oai:scielo:S0100-879X1998000600017
network_acronym_str ABDC-1
network_name_str Brazilian Journal of Medical and Biological Research
repository_id_str
spelling Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake ratshypervolemiablood volumegastrointestinal transitratsgastrointestinal motilityThe present study evaluates the effect of blood volume expansion on the gastrointestinal transit of a charchoal meal (2.5 ml of an aqueous suspension consisting of 5% charcoal and 5% gum arabic) in awake male Wistar rats (200-270 g). On the day before the experiments, the rats were anesthetized with ether, submitted to left jugular vein cannulation and fasted with water ad libitum until 2 h before the gastrointestinal transit measurement. Blood volume expansion by iv infusion of 1 ml/min Ringer bicarbonate in volumes of 3, 4 or 5% body weight delayed gastrointestinal transit at 10 min after test meal administration by 21.3-26.7% (P<0.05), but no effect was observed after 1 or 2% body weight expansion. The effect of blood volume expansion (up to 5% body weight) on gastrointestinal transit lasted for at least 60 min (P<0.05). Mean arterial pressure increased transiently and central venous pressure increased and hematocrit decreased (P<0.05). Subdiaphragmatic vagotomy and yohimbine (3 mg/kg) prevented the delay caused by expansion on gastrointestinal transit, while atropine (0.5 mg/kg), L-NAME (2 mg/kg), hexamethonium (10 mg/kg), prazosin (1 mg/kg) or propranolol (2 mg/kg) were ineffective. These data show that blood volume expansion delays the gastrointestinal transit of a charcoal meal and that vagal and yohimbine-sensitive pathways appear to be involved in this phenomenon. The delay in gastrointestinal transit observed here, taken together with the modifications of gastrointestinal permeability to salt and water reported by others, may be part of the mechanisms involved in liquid excess management.Associação Brasileira de Divulgação Científica1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017Brazilian Journal of Medical and Biological Research v.31 n.6 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998000600017info:eu-repo/semantics/openAccessde-Oliveira,G.R.Gondim,F. de-A.A.da-Graça,J.R.V.Xavier-Neto,J.Dantas,R.P.Gondim,R.B.M.Santos,A.A.Rola,F.H.eng1998-10-06T00:00:00Zoai:scielo:S0100-879X1998000600017Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-06T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
title Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
spellingShingle Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
de-Oliveira,G.R.
hypervolemia
blood volume
gastrointestinal transit
rats
gastrointestinal motility
title_short Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
title_full Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
title_fullStr Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
title_full_unstemmed Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
title_sort Acute blood volume expansion delays the gastrointestinal transit of a charcoal meal in awake rats
author de-Oliveira,G.R.
author_facet de-Oliveira,G.R.
Gondim,F. de-A.A.
da-Graça,J.R.V.
Xavier-Neto,J.
Dantas,R.P.
Gondim,R.B.M.
Santos,A.A.
Rola,F.H.
author_role author
author2 Gondim,F. de-A.A.
da-Graça,J.R.V.
Xavier-Neto,J.
Dantas,R.P.
Gondim,R.B.M.
Santos,A.A.
Rola,F.H.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv de-Oliveira,G.R.
Gondim,F. de-A.A.
da-Graça,J.R.V.
Xavier-Neto,J.
Dantas,R.P.
Gondim,R.B.M.
Santos,A.A.
Rola,F.H.
dc.subject.por.fl_str_mv hypervolemia
blood volume
gastrointestinal transit
rats
gastrointestinal motility
topic hypervolemia
blood volume
gastrointestinal transit
rats
gastrointestinal motility
description The present study evaluates the effect of blood volume expansion on the gastrointestinal transit of a charchoal meal (2.5 ml of an aqueous suspension consisting of 5% charcoal and 5% gum arabic) in awake male Wistar rats (200-270 g). On the day before the experiments, the rats were anesthetized with ether, submitted to left jugular vein cannulation and fasted with water ad libitum until 2 h before the gastrointestinal transit measurement. Blood volume expansion by iv infusion of 1 ml/min Ringer bicarbonate in volumes of 3, 4 or 5% body weight delayed gastrointestinal transit at 10 min after test meal administration by 21.3-26.7% (P<0.05), but no effect was observed after 1 or 2% body weight expansion. The effect of blood volume expansion (up to 5% body weight) on gastrointestinal transit lasted for at least 60 min (P<0.05). Mean arterial pressure increased transiently and central venous pressure increased and hematocrit decreased (P<0.05). Subdiaphragmatic vagotomy and yohimbine (3 mg/kg) prevented the delay caused by expansion on gastrointestinal transit, while atropine (0.5 mg/kg), L-NAME (2 mg/kg), hexamethonium (10 mg/kg), prazosin (1 mg/kg) or propranolol (2 mg/kg) were ineffective. These data show that blood volume expansion delays the gastrointestinal transit of a charcoal meal and that vagal and yohimbine-sensitive pathways appear to be involved in this phenomenon. The delay in gastrointestinal transit observed here, taken together with the modifications of gastrointestinal permeability to salt and water reported by others, may be part of the mechanisms involved in liquid excess management.
publishDate 1998
dc.date.none.fl_str_mv 1998-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X1998000600017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.31 n.6 1998
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
_version_ 1754302929297211392