Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200009 |
Resumo: | We investigated the vascular responses and the blood pressure reducing effects of different fractions obtained from the methanol extract of Loranthus ferrugineus Roxb. (F. Loranthaceae). By means of solvent-solvent extraction, L. ferrugineus methanol extract (LFME) was successively fractionated with chloroform, ethyl acetate and n-butanol. The ability of these LFME fractions to relax vascular smooth muscle against phenylephrine (PE)- and KCl-induced contractions in isolated rat aortic rings was determined. In another set of experiments, LFME fractions were tested for blood pressure lowering activity in anesthetized adult male Sprague-Dawley rats (250-300 g, 14-18 weeks). The n-butanol fraction of LFME (NBF-LFME) produced a significant concentration-dependent inhibition of PE- and KCl-induced aortic ring contractions compared to other fractions. Moreover, NBF-LFME had a significantly higher relaxant effect against PE- than against high K+-induced contractions. In anesthetized Sprague-Dawley rats, NBF-LFME significantly lowered blood pressure in a dose-dependent manner and with a relatively longer duration of action compared to the other fractions. HPLC, UV and IR spectra suggested the presence of terpenoid constituents in both LFME and NBF-LFME. Accordingly, we conclude that NBF-LFME is the most potent fraction producing a concentration-dependent relaxation in vascular smooth muscle in vitro and a dose-dependent blood pressure lowering activity in vivo. The cardiovascular effects of NBF-LFME are most likely attributable to its terpenoid content. |
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Brazilian Journal of Medical and Biological Research |
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Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb.Loranthus ferrugineusn-butanol fractionCardiovascular effectAortic ringVasorelaxationTerpenoidWe investigated the vascular responses and the blood pressure reducing effects of different fractions obtained from the methanol extract of Loranthus ferrugineus Roxb. (F. Loranthaceae). By means of solvent-solvent extraction, L. ferrugineus methanol extract (LFME) was successively fractionated with chloroform, ethyl acetate and n-butanol. The ability of these LFME fractions to relax vascular smooth muscle against phenylephrine (PE)- and KCl-induced contractions in isolated rat aortic rings was determined. In another set of experiments, LFME fractions were tested for blood pressure lowering activity in anesthetized adult male Sprague-Dawley rats (250-300 g, 14-18 weeks). The n-butanol fraction of LFME (NBF-LFME) produced a significant concentration-dependent inhibition of PE- and KCl-induced aortic ring contractions compared to other fractions. Moreover, NBF-LFME had a significantly higher relaxant effect against PE- than against high K+-induced contractions. In anesthetized Sprague-Dawley rats, NBF-LFME significantly lowered blood pressure in a dose-dependent manner and with a relatively longer duration of action compared to the other fractions. HPLC, UV and IR spectra suggested the presence of terpenoid constituents in both LFME and NBF-LFME. Accordingly, we conclude that NBF-LFME is the most potent fraction producing a concentration-dependent relaxation in vascular smooth muscle in vitro and a dose-dependent blood pressure lowering activity in vivo. The cardiovascular effects of NBF-LFME are most likely attributable to its terpenoid content.Associação Brasileira de Divulgação Científica2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200009Brazilian Journal of Medical and Biological Research v.43 n.2 2010reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2010005000002info:eu-repo/semantics/openAccessAmeer,O.Z.Salman,I.M.Siddiqui,M.J.A.Yam,M.F.Sriramaneni,R.N.Sadikun,A.Ismail,Z.Shah,A.M.Asmawi,M.Z.eng2010-02-22T00:00:00Zoai:scielo:S0100-879X2010000200009Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2010-02-22T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
title |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
spellingShingle |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. Ameer,O.Z. Loranthus ferrugineus n-butanol fraction Cardiovascular effect Aortic ring Vasorelaxation Terpenoid |
title_short |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
title_full |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
title_fullStr |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
title_full_unstemmed |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
title_sort |
Cardiovascular activity of the n-butanol fraction of the methanol extract of Loranthus ferrugineus Roxb. |
author |
Ameer,O.Z. |
author_facet |
Ameer,O.Z. Salman,I.M. Siddiqui,M.J.A. Yam,M.F. Sriramaneni,R.N. Sadikun,A. Ismail,Z. Shah,A.M. Asmawi,M.Z. |
author_role |
author |
author2 |
Salman,I.M. Siddiqui,M.J.A. Yam,M.F. Sriramaneni,R.N. Sadikun,A. Ismail,Z. Shah,A.M. Asmawi,M.Z. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ameer,O.Z. Salman,I.M. Siddiqui,M.J.A. Yam,M.F. Sriramaneni,R.N. Sadikun,A. Ismail,Z. Shah,A.M. Asmawi,M.Z. |
dc.subject.por.fl_str_mv |
Loranthus ferrugineus n-butanol fraction Cardiovascular effect Aortic ring Vasorelaxation Terpenoid |
topic |
Loranthus ferrugineus n-butanol fraction Cardiovascular effect Aortic ring Vasorelaxation Terpenoid |
description |
We investigated the vascular responses and the blood pressure reducing effects of different fractions obtained from the methanol extract of Loranthus ferrugineus Roxb. (F. Loranthaceae). By means of solvent-solvent extraction, L. ferrugineus methanol extract (LFME) was successively fractionated with chloroform, ethyl acetate and n-butanol. The ability of these LFME fractions to relax vascular smooth muscle against phenylephrine (PE)- and KCl-induced contractions in isolated rat aortic rings was determined. In another set of experiments, LFME fractions were tested for blood pressure lowering activity in anesthetized adult male Sprague-Dawley rats (250-300 g, 14-18 weeks). The n-butanol fraction of LFME (NBF-LFME) produced a significant concentration-dependent inhibition of PE- and KCl-induced aortic ring contractions compared to other fractions. Moreover, NBF-LFME had a significantly higher relaxant effect against PE- than against high K+-induced contractions. In anesthetized Sprague-Dawley rats, NBF-LFME significantly lowered blood pressure in a dose-dependent manner and with a relatively longer duration of action compared to the other fractions. HPLC, UV and IR spectra suggested the presence of terpenoid constituents in both LFME and NBF-LFME. Accordingly, we conclude that NBF-LFME is the most potent fraction producing a concentration-dependent relaxation in vascular smooth muscle in vitro and a dose-dependent blood pressure lowering activity in vivo. The cardiovascular effects of NBF-LFME are most likely attributable to its terpenoid content. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2010005000002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.43 n.2 2010 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302938594934784 |