CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells

Detalhes bibliográficos
Autor(a) principal: Mudado,M.A.
Data de Publicação: 2004
Outros Autores: Rodrigues,A.L., Prado,V.F., Beirão,P.S.L., Cruz,J.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020
Resumo: T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of -120 mV, N = 15), and -27.0 ± 0.97 and 7.5 ± 0.7 mV (pre-pulse of -40 mV, N = 9). The 8-mV shift in the activation mid-point was statistically significant (P < 0.05). The tail currents decayed bi-exponentially suggesting two different T-type Ca2+ channel populations. RT-PCR revealed the presence of a1G (CaV3.1) and a1I (CaV3.3) T-type Ca2+ channel mRNA transcripts.
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spelling CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cellsCalcium channelT-typeElectrophysiologyRT-PCRGH3 cellsT-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of -120 mV, N = 15), and -27.0 ± 0.97 and 7.5 ± 0.7 mV (pre-pulse of -40 mV, N = 9). The 8-mV shift in the activation mid-point was statistically significant (P < 0.05). The tail currents decayed bi-exponentially suggesting two different T-type Ca2+ channel populations. RT-PCR revealed the presence of a1G (CaV3.1) and a1I (CaV3.3) T-type Ca2+ channel mRNA transcripts.Associação Brasileira de Divulgação Científica2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020Brazilian Journal of Medical and Biological Research v.37 n.6 2004reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2004000600020info:eu-repo/semantics/openAccessMudado,M.A.Rodrigues,A.L.Prado,V.F.Beirão,P.S.L.Cruz,J.S.eng2004-05-27T00:00:00Zoai:scielo:S0100-879X2004000600020Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2004-05-27T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
title CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
spellingShingle CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
Mudado,M.A.
Calcium channel
T-type
Electrophysiology
RT-PCR
GH3 cells
title_short CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
title_full CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
title_fullStr CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
title_full_unstemmed CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
title_sort CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
author Mudado,M.A.
author_facet Mudado,M.A.
Rodrigues,A.L.
Prado,V.F.
Beirão,P.S.L.
Cruz,J.S.
author_role author
author2 Rodrigues,A.L.
Prado,V.F.
Beirão,P.S.L.
Cruz,J.S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mudado,M.A.
Rodrigues,A.L.
Prado,V.F.
Beirão,P.S.L.
Cruz,J.S.
dc.subject.por.fl_str_mv Calcium channel
T-type
Electrophysiology
RT-PCR
GH3 cells
topic Calcium channel
T-type
Electrophysiology
RT-PCR
GH3 cells
description T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of -120 mV, N = 15), and -27.0 ± 0.97 and 7.5 ± 0.7 mV (pre-pulse of -40 mV, N = 9). The 8-mV shift in the activation mid-point was statistically significant (P < 0.05). The tail currents decayed bi-exponentially suggesting two different T-type Ca2+ channel populations. RT-PCR revealed the presence of a1G (CaV3.1) and a1I (CaV3.3) T-type Ca2+ channel mRNA transcripts.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2004000600020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.37 n.6 2004
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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