Impact of the contamination time by Escherichia coli on biofilm formation in surgical instruments

• Autor(a) principal: Evangelista,Síntia de Souza
• Data de Publicação: 2021
• Outros Autores: Santos,Simone Gonçalves dos, Oliveira,Adriana Cristina de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Enfermagem (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71672021000300170
• topic: Biofilms; Escherichia coli; Surgical Instruments; Patient Safety; Equipment Contamination

ABSTRACT Objectives: to evaluate the microbial load and adherence of Escherichia coli in different areas of the surgical instrument surface exposed to experimental contamination over time. Methods: experimental study in which fragments of crile forceps (serrated, rod and rack) were contaminated by immersion in Tryptic Soy Broth, containing 106 CFU/mL of E. coli, for 1, 2, 4, 6, 8, 12 and 24 hours. Microbial load and bacterial adherence were evaluated using microbiological culture and scanning electron microscopy, respectively. Results: there was an increase in the microbial load on the surgical instrument, proportional to the contamination interval, ranging from 102 after 1 hour to 105 CFU/cm2 in 24 hours. The presence of exopolysaccharide was detected after two hours of contamination. Conclusions: microbial load and adhesion of E. coli increased over time, reaching 105 CFU/cm2 after 24 hours of contamination, starting biofilm formation after two hours.