Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank

Detalhes bibliográficos
Autor(a) principal: Demenech, Luciana Stadler
Data de Publicação: 2017
Outros Autores: Tomazinho, Flávia Sens Fagundes, Tomazinho, Paulo Henrique, Gabardo, Marilisa Carneiro Leão, Baratto-Filho, Flares
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Revista da ABENO (Online)
Texto Completo: https://revabeno.emnuvens.com.br/revabeno/article/view/427
Resumo: The importance of a human teeth bank in an institution is justified and it is advantageous, since it allows the maximum approximation of reality when working with the extracted organ. This study aimed to evaluate methods for maintaining the sterility of extracted human teeth during storage. A total of 72 human incisors extracted in the undergraduate and graduate clinics of Universidade Positivo (Curitiba, PR, Brazil) were used in this study. The teeth were provided by patients, who signed a donation form. After all teeth were subjected to cleaning and disinfection procedures, 36 teeth were autoclaved as well. Teeth were then stored in various solutions for periods of 15 and 120 days. Microbiological tests were conducted to determine which method or storage promoted maintenance of sterility. Better results were obtained for teeth - including autoclaved teeth - stored in Incidin Extra N®, formaldehyde, and alcohol. The solutions analyzed over the proposed time periods have been shown to prevent microbial growth. Results of this study will aid in developing a protocol of processing for extracted human dental teeth to be stored in a tooth bank.
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spelling Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bankAvaliação de métodos de manutenção da esterilidade do órgão dental humano extraído para armazenamento em banco de dentesDentistry. Biosecurity. Sterilization. Tooth. Microbiological Techniques.Odontologia. Biossegurança. Esterilização. Dente. Técnicas microbiológicas.The importance of a human teeth bank in an institution is justified and it is advantageous, since it allows the maximum approximation of reality when working with the extracted organ. This study aimed to evaluate methods for maintaining the sterility of extracted human teeth during storage. A total of 72 human incisors extracted in the undergraduate and graduate clinics of Universidade Positivo (Curitiba, PR, Brazil) were used in this study. The teeth were provided by patients, who signed a donation form. After all teeth were subjected to cleaning and disinfection procedures, 36 teeth were autoclaved as well. Teeth were then stored in various solutions for periods of 15 and 120 days. Microbiological tests were conducted to determine which method or storage promoted maintenance of sterility. Better results were obtained for teeth - including autoclaved teeth - stored in Incidin Extra N®, formaldehyde, and alcohol. The solutions analyzed over the proposed time periods have been shown to prevent microbial growth. Results of this study will aid in developing a protocol of processing for extracted human dental teeth to be stored in a tooth bank.A importância de um banco de dentes humanos em uma instituição de se justifica e se mostra vantajosa, uma vez que permite a máxima aproximação da realidade ao se trabalhar com o órgão extraído. Este estudo teve o propósito de avaliar métodos de manutenção da esterilidade do órgão dental humano extraído armazenado. Foram utilizados 72 dentes incisivos humanos extraídos, obtidos em clínicas de graduação e de pós-graduação da Universidade Positivo, Curitiba, PR, Brasil. Os elementos dentários foram cedidos pelos pacientes, por meio de termo de doação devidamente assinado. Após os procedimentos de limpeza e de desinfecção, 36 dentes foram esterilizados em autoclave e 36 foram somente limpos. Os dentes foram, então, armazenados em recipientes contendo o método (autoclavagem ou limpeza) ou a solução de escolha, por um período de 15 e 120 dias. Testes microbiológicos foram realizados a fim de determinar qual método ou solução de armazenamento promove a manutenção da esterilidade no tempo determinado. Melhores desempenhos foram observados quando as amostras foram armazenadas em Incidin Extra N®, formol e álcool, mesmo para os dentes não autoclavados. As substâncias em análise nos períodos propostos se mostraram capazes de impedir o crescimento microbiano. Este experimento poderá auxiliar o desenvolvimento de um protocolo de processamento e de administração do órgão dental humano extraído em um banco de dentes.Associação Brasileira de Ensino Odontológico2017-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revabeno.emnuvens.com.br/revabeno/article/view/42710.30979/rev.abeno.v17i3.427Revista da ABENO; Vol. 17 No. 3 (2017); 55-65Revista da ABENO; Vol. 17 Núm. 3 (2017); 55-65Revista da ABENO; v. 17 n. 3 (2017); 55-652595-02741679-5954reponame:Revista da ABENO (Online)instname:Associação Brasileira de Ensino Odontológico (ABENO)instacron:ABENOporenghttps://revabeno.emnuvens.com.br/revabeno/article/view/427/324https://revabeno.emnuvens.com.br/revabeno/article/view/427/323Copyright (c) 2017 Revista da ABENOinfo:eu-repo/semantics/openAccessDemenech, Luciana StadlerTomazinho, Flávia Sens FagundesTomazinho, Paulo HenriqueGabardo, Marilisa Carneiro LeãoBaratto-Filho, Flares2017-09-15T09:56:51Zoai:ojs.revabeno.emnuvens.com.br:article/427Revistahttps://revabeno.emnuvens.com.br/revabeno/indexONGhttps://revabeno.emnuvens.com.br/revabeno/oairevabeno@gmail.com2595-02741679-5954opendoar:2023-01-13T09:48:02.186760Revista da ABENO (Online) - Associação Brasileira de Ensino Odontológico (ABENO)false
dc.title.none.fl_str_mv Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
Avaliação de métodos de manutenção da esterilidade do órgão dental humano extraído para armazenamento em banco de dentes
title Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
spellingShingle Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
Demenech, Luciana Stadler
Dentistry. Biosecurity. Sterilization. Tooth. Microbiological Techniques.
Odontologia. Biossegurança. Esterilização. Dente. Técnicas microbiológicas.
title_short Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
title_full Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
title_fullStr Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
title_full_unstemmed Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
title_sort Evaluation of methods for maintaining sterility of the extracted human dental organ during storage in a tooth bank
author Demenech, Luciana Stadler
author_facet Demenech, Luciana Stadler
Tomazinho, Flávia Sens Fagundes
Tomazinho, Paulo Henrique
Gabardo, Marilisa Carneiro Leão
Baratto-Filho, Flares
author_role author
author2 Tomazinho, Flávia Sens Fagundes
Tomazinho, Paulo Henrique
Gabardo, Marilisa Carneiro Leão
Baratto-Filho, Flares
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Demenech, Luciana Stadler
Tomazinho, Flávia Sens Fagundes
Tomazinho, Paulo Henrique
Gabardo, Marilisa Carneiro Leão
Baratto-Filho, Flares
dc.subject.por.fl_str_mv Dentistry. Biosecurity. Sterilization. Tooth. Microbiological Techniques.
Odontologia. Biossegurança. Esterilização. Dente. Técnicas microbiológicas.
topic Dentistry. Biosecurity. Sterilization. Tooth. Microbiological Techniques.
Odontologia. Biossegurança. Esterilização. Dente. Técnicas microbiológicas.
description The importance of a human teeth bank in an institution is justified and it is advantageous, since it allows the maximum approximation of reality when working with the extracted organ. This study aimed to evaluate methods for maintaining the sterility of extracted human teeth during storage. A total of 72 human incisors extracted in the undergraduate and graduate clinics of Universidade Positivo (Curitiba, PR, Brazil) were used in this study. The teeth were provided by patients, who signed a donation form. After all teeth were subjected to cleaning and disinfection procedures, 36 teeth were autoclaved as well. Teeth were then stored in various solutions for periods of 15 and 120 days. Microbiological tests were conducted to determine which method or storage promoted maintenance of sterility. Better results were obtained for teeth - including autoclaved teeth - stored in Incidin Extra N®, formaldehyde, and alcohol. The solutions analyzed over the proposed time periods have been shown to prevent microbial growth. Results of this study will aid in developing a protocol of processing for extracted human dental teeth to be stored in a tooth bank.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revabeno.emnuvens.com.br/revabeno/article/view/427
10.30979/rev.abeno.v17i3.427
url https://revabeno.emnuvens.com.br/revabeno/article/view/427
identifier_str_mv 10.30979/rev.abeno.v17i3.427
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://revabeno.emnuvens.com.br/revabeno/article/view/427/324
https://revabeno.emnuvens.com.br/revabeno/article/view/427/323
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista da ABENO
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista da ABENO
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Ensino Odontológico
publisher.none.fl_str_mv Associação Brasileira de Ensino Odontológico
dc.source.none.fl_str_mv Revista da ABENO; Vol. 17 No. 3 (2017); 55-65
Revista da ABENO; Vol. 17 Núm. 3 (2017); 55-65
Revista da ABENO; v. 17 n. 3 (2017); 55-65
2595-0274
1679-5954
reponame:Revista da ABENO (Online)
instname:Associação Brasileira de Ensino Odontológico (ABENO)
instacron:ABENO
instname_str Associação Brasileira de Ensino Odontológico (ABENO)
instacron_str ABENO
institution ABENO
reponame_str Revista da ABENO (Online)
collection Revista da ABENO (Online)
repository.name.fl_str_mv Revista da ABENO (Online) - Associação Brasileira de Ensino Odontológico (ABENO)
repository.mail.fl_str_mv revabeno@gmail.com
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