Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300739 |
Resumo: | ABSTRACT In this study, a high salt-tolerant and glyphosate-degrading strain named BZ8 was isolated from activated sludge. According to 16S rDNA sequencing methods, morphological, physiological and biochemical analysis, strain BZ8 was identified as Agrobacterium tumefaciens. The growth and glyphosate-degrading capability of A. tumefaciens BZ8 were investigated and the results showed that the optimum conditions for glyphosate degradation under 6% of NaCl concentration were found as follows: inoculation size of 10% (v/v), incubation temperature 37℃ and initial pH of 5.0. Salt tolerance test showed that A. tumefaciens BZ8 grew well and could thoroughly degrade 2000 mg/L glyphosate in 36 h if the concentration of NaCl was lower than 6%, while the degradation rate decreased gradually with increasing NaCl concentration. But the glyphosate degradation rate could still reach 62% when the salt concentration was 8%. In addition, the kinetic parameters for A. tumefaciens BZ8 grown on 100-2800 mg/L glyphosate according to Haldane’s model could predict the cell growth tendency successfully. These results showed that A. tumefaciens BZ8 could be used to control glyphosate wastewater with high salt content. Therefore, it has potential application. |
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Brazilian Journal of Chemical Engineering |
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Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8salt-tolerantglyphosate-degrading strainidentificationgrowth kineticsABSTRACT In this study, a high salt-tolerant and glyphosate-degrading strain named BZ8 was isolated from activated sludge. According to 16S rDNA sequencing methods, morphological, physiological and biochemical analysis, strain BZ8 was identified as Agrobacterium tumefaciens. The growth and glyphosate-degrading capability of A. tumefaciens BZ8 were investigated and the results showed that the optimum conditions for glyphosate degradation under 6% of NaCl concentration were found as follows: inoculation size of 10% (v/v), incubation temperature 37℃ and initial pH of 5.0. Salt tolerance test showed that A. tumefaciens BZ8 grew well and could thoroughly degrade 2000 mg/L glyphosate in 36 h if the concentration of NaCl was lower than 6%, while the degradation rate decreased gradually with increasing NaCl concentration. But the glyphosate degradation rate could still reach 62% when the salt concentration was 8%. In addition, the kinetic parameters for A. tumefaciens BZ8 grown on 100-2800 mg/L glyphosate according to Haldane’s model could predict the cell growth tendency successfully. These results showed that A. tumefaciens BZ8 could be used to control glyphosate wastewater with high salt content. Therefore, it has potential application.Brazilian Society of Chemical Engineering2017-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300739Brazilian Journal of Chemical Engineering v.34 n.3 2017reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20170343s20150803info:eu-repo/semantics/openAccessYan,ShoubaoZhao,WeiShi,CuieTong,QianqianZhai,ZhijunWang,Yuneng2018-02-01T00:00:00Zoai:scielo:S0104-66322017000300739Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2018-02-01T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
title |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
spellingShingle |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 Yan,Shoubao salt-tolerant glyphosate-degrading strain identification growth kinetics |
title_short |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
title_full |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
title_fullStr |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
title_full_unstemmed |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
title_sort |
Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8 |
author |
Yan,Shoubao |
author_facet |
Yan,Shoubao Zhao,Wei Shi,Cuie Tong,Qianqian Zhai,Zhijun Wang,Yun |
author_role |
author |
author2 |
Zhao,Wei Shi,Cuie Tong,Qianqian Zhai,Zhijun Wang,Yun |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Yan,Shoubao Zhao,Wei Shi,Cuie Tong,Qianqian Zhai,Zhijun Wang,Yun |
dc.subject.por.fl_str_mv |
salt-tolerant glyphosate-degrading strain identification growth kinetics |
topic |
salt-tolerant glyphosate-degrading strain identification growth kinetics |
description |
ABSTRACT In this study, a high salt-tolerant and glyphosate-degrading strain named BZ8 was isolated from activated sludge. According to 16S rDNA sequencing methods, morphological, physiological and biochemical analysis, strain BZ8 was identified as Agrobacterium tumefaciens. The growth and glyphosate-degrading capability of A. tumefaciens BZ8 were investigated and the results showed that the optimum conditions for glyphosate degradation under 6% of NaCl concentration were found as follows: inoculation size of 10% (v/v), incubation temperature 37℃ and initial pH of 5.0. Salt tolerance test showed that A. tumefaciens BZ8 grew well and could thoroughly degrade 2000 mg/L glyphosate in 36 h if the concentration of NaCl was lower than 6%, while the degradation rate decreased gradually with increasing NaCl concentration. But the glyphosate degradation rate could still reach 62% when the salt concentration was 8%. In addition, the kinetic parameters for A. tumefaciens BZ8 grown on 100-2800 mg/L glyphosate according to Haldane’s model could predict the cell growth tendency successfully. These results showed that A. tumefaciens BZ8 could be used to control glyphosate wastewater with high salt content. Therefore, it has potential application. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300739 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000300739 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20170343s20150803 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.34 n.3 2017 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213175530618880 |