Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Rossi,F. G.
Data de Publicação: 2009
Outros Autores: Silva,D. P., Almeida e Silva,J. B., Taqueda,M. E., Vitolo,M., Pessoa-Jr,A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001
Resumo: The objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme.
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spelling Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiaeSaccharomyces cerevisiaeFermentationEnzyme productionGlucose-6-phosphate dehydrogenaseStrain genetically modifiedThe objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme.Brazilian Society of Chemical Engineering2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001Brazilian Journal of Chemical Engineering v.26 n.1 2009reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322009000100001info:eu-repo/semantics/openAccessRossi,F. G.Silva,D. P.Almeida e Silva,J. B.Taqueda,M. E.Vitolo,M.Pessoa-Jr,A.eng2009-03-10T00:00:00Zoai:scielo:S0104-66322009000100001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2009-03-10T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
title Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
spellingShingle Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
Rossi,F. G.
Saccharomyces cerevisiae
Fermentation
Enzyme production
Glucose-6-phosphate dehydrogenase
Strain genetically modified
title_short Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
title_full Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
title_fullStr Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
title_full_unstemmed Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
title_sort Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
author Rossi,F. G.
author_facet Rossi,F. G.
Silva,D. P.
Almeida e Silva,J. B.
Taqueda,M. E.
Vitolo,M.
Pessoa-Jr,A.
author_role author
author2 Silva,D. P.
Almeida e Silva,J. B.
Taqueda,M. E.
Vitolo,M.
Pessoa-Jr,A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rossi,F. G.
Silva,D. P.
Almeida e Silva,J. B.
Taqueda,M. E.
Vitolo,M.
Pessoa-Jr,A.
dc.subject.por.fl_str_mv Saccharomyces cerevisiae
Fermentation
Enzyme production
Glucose-6-phosphate dehydrogenase
Strain genetically modified
topic Saccharomyces cerevisiae
Fermentation
Enzyme production
Glucose-6-phosphate dehydrogenase
Strain genetically modified
description The objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322009000100001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.26 n.1 2009
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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