Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001 |
Resumo: | The objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme. |
id |
ABEQ-1_1714ff3cb8b1b66a7479d86493a58ae6 |
---|---|
oai_identifier_str |
oai:scielo:S0104-66322009000100001 |
network_acronym_str |
ABEQ-1 |
network_name_str |
Brazilian Journal of Chemical Engineering |
repository_id_str |
|
spelling |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiaeSaccharomyces cerevisiaeFermentationEnzyme productionGlucose-6-phosphate dehydrogenaseStrain genetically modifiedThe objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme.Brazilian Society of Chemical Engineering2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001Brazilian Journal of Chemical Engineering v.26 n.1 2009reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322009000100001info:eu-repo/semantics/openAccessRossi,F. G.Silva,D. P.Almeida e Silva,J. B.Taqueda,M. E.Vitolo,M.Pessoa-Jr,A.eng2009-03-10T00:00:00Zoai:scielo:S0104-66322009000100001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2009-03-10T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
title |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
spellingShingle |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae Rossi,F. G. Saccharomyces cerevisiae Fermentation Enzyme production Glucose-6-phosphate dehydrogenase Strain genetically modified |
title_short |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
title_full |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
title_fullStr |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
title_full_unstemmed |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
title_sort |
Effect of cultivation conditions on glucose-6-phosphate dehydrogenase production by genetically modified Saccharomyces cerevisiae |
author |
Rossi,F. G. |
author_facet |
Rossi,F. G. Silva,D. P. Almeida e Silva,J. B. Taqueda,M. E. Vitolo,M. Pessoa-Jr,A. |
author_role |
author |
author2 |
Silva,D. P. Almeida e Silva,J. B. Taqueda,M. E. Vitolo,M. Pessoa-Jr,A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rossi,F. G. Silva,D. P. Almeida e Silva,J. B. Taqueda,M. E. Vitolo,M. Pessoa-Jr,A. |
dc.subject.por.fl_str_mv |
Saccharomyces cerevisiae Fermentation Enzyme production Glucose-6-phosphate dehydrogenase Strain genetically modified |
topic |
Saccharomyces cerevisiae Fermentation Enzyme production Glucose-6-phosphate dehydrogenase Strain genetically modified |
description |
The objective of this research was to improve Glucose-6-phosphate dehydrogenase (G6PD) production by Saccharomyces cerevisiae W303-181, which carry the plasmid YEpPGK-G6PD, by varying the following cultivation conditions: pH value (4.8, 5.7 and 6.6); inoculum concentration (0.1, 0.6 and 1.1 g/L) and initial glucose concentration (20.0, 30.0 and 40.0 g/L). The effect of those variables on G6PD production capability was studied by the application of response surface statistical analysis. The results showed that the highest G6PD production (1594.2 U/L), specific activity (1189.7 U/g cell) and productivity (45.6 U/L.h) occurred at pH 4.8, inoculum concentration of 0.1 g/L and initial glucose concentration of 20.0 g/L, under agitation of 150 rpm at 30ºC after 36 h. In this work, the strain expressed about 21 fold more activity than the wild S. cerevisiae strain, being an attractive and promising new source of this enzyme. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322009000100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322009000100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.26 n.1 2009 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213172716240896 |