Nuclease p1 immobilized on deae cellulose

Detalhes bibliográficos
Autor(a) principal: Shi,Lu-E
Data de Publicação: 2010
Outros Autores: Yi,Yu, Tang,Zhen-Xing, Xiong,Wen-Yue, Mei,Jiang-Feng, Ying,Guo-Qing
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100003
Resumo: Effects of various factors, such as pH, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. The immobilization conditions were optimized by orthogonal experiments. Characterizations of immobilized nuclease p1 were also evaluated. Through orthogonal optimization, the optimal immobilization conditions were as follows: pH 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. Optimal pH of immobilized enzyme was 5.8. Optimal temperature of immobilized enzyme was 70ºC. Thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on DEAE cellulose. Michaelis constant Km of immobilized enzyme at 69ºC was found to be 27.21 g/l by the Lineweaver-Burk plot.
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spelling Nuclease p1 immobilized on deae celluloseDEAE celluloseNuclease p1ImmobilizationCharacterizationEffects of various factors, such as pH, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. The immobilization conditions were optimized by orthogonal experiments. Characterizations of immobilized nuclease p1 were also evaluated. Through orthogonal optimization, the optimal immobilization conditions were as follows: pH 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. Optimal pH of immobilized enzyme was 5.8. Optimal temperature of immobilized enzyme was 70ºC. Thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on DEAE cellulose. Michaelis constant Km of immobilized enzyme at 69ºC was found to be 27.21 g/l by the Lineweaver-Burk plot.Brazilian Society of Chemical Engineering2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100003Brazilian Journal of Chemical Engineering v.27 n.1 2010reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322010000100003info:eu-repo/semantics/openAccessShi,Lu-EYi,YuTang,Zhen-XingXiong,Wen-YueMei,Jiang-FengYing,Guo-Qingeng2010-04-14T00:00:00Zoai:scielo:S0104-66322010000100003Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2010-04-14T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Nuclease p1 immobilized on deae cellulose
title Nuclease p1 immobilized on deae cellulose
spellingShingle Nuclease p1 immobilized on deae cellulose
Shi,Lu-E
DEAE cellulose
Nuclease p1
Immobilization
Characterization
title_short Nuclease p1 immobilized on deae cellulose
title_full Nuclease p1 immobilized on deae cellulose
title_fullStr Nuclease p1 immobilized on deae cellulose
title_full_unstemmed Nuclease p1 immobilized on deae cellulose
title_sort Nuclease p1 immobilized on deae cellulose
author Shi,Lu-E
author_facet Shi,Lu-E
Yi,Yu
Tang,Zhen-Xing
Xiong,Wen-Yue
Mei,Jiang-Feng
Ying,Guo-Qing
author_role author
author2 Yi,Yu
Tang,Zhen-Xing
Xiong,Wen-Yue
Mei,Jiang-Feng
Ying,Guo-Qing
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Shi,Lu-E
Yi,Yu
Tang,Zhen-Xing
Xiong,Wen-Yue
Mei,Jiang-Feng
Ying,Guo-Qing
dc.subject.por.fl_str_mv DEAE cellulose
Nuclease p1
Immobilization
Characterization
topic DEAE cellulose
Nuclease p1
Immobilization
Characterization
description Effects of various factors, such as pH, ionic strength, glutaraldehyde concentration, enzyme amount and immobilization time, on enzyme activity were investigated. The immobilization conditions were optimized by orthogonal experiments. Characterizations of immobilized nuclease p1 were also evaluated. Through orthogonal optimization, the optimal immobilization conditions were as follows: pH 5.6, ionic strength 0.125, glutaraldehyde concentration 0.20% and immobilization time 2.0 h. Optimal pH of immobilized enzyme was 5.8. Optimal temperature of immobilized enzyme was 70ºC. Thermal, operational and storage stabilities of the enzyme were improved after it was immobilized on DEAE cellulose. Michaelis constant Km of immobilized enzyme at 69ºC was found to be 27.21 g/l by the Lineweaver-Burk plot.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322010000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.27 n.1 2010
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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