Characterization of chemically modified chitosan microspheres as adsorbents using standard Proteins (bovine serum albumin and lysozyme)

• Autor(a) principal: Torres,M. A.
• Data de Publicação: 2007
• Outros Autores: Beppu,M. M., Santana,C. C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Chemical Engineering
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322007000300003
• topic: Chitosan microspheres BSA lysozyme adsorption

Chitosan microspheres with a mean size of 140 ± 119 µm were produced by the spray and coagulation methods. The microspheres were chemically modified using the following routes: a) crosslinking with glutaraldehyde b) crosslinking with epychlorohydrin and c) acetylation. For investigation of their ability as adsorbents, the following standard proteins were chosen as adsorbates: bovine serum albumin - BSA (pI = 4.8 and MW = 66 kDa) and lysozyme (pI = 11 and MW = 14 kDa). The adsorption experiments were performed using a static method. The adsorption media and equilibrium concentration of adsorbates were varied in the ranges of pH 4-11 and 0.07-0.70 mg.ml-1, respectively. The maximum adsorption capacities (q m) and the constant of the Langmuir model (Ks) were shown to be dependent on charge interactions and on the kind of treatment performed on chitosan microspheres. The satisfactory fit of a kinetic model to the experimental data shows that the step that controls the adsorption kinetics is probably the initial adsorbate transport.