ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)

Detalhes bibliográficos
Autor(a) principal: Silva Júnior,Benesval L.
Data de Publicação: 2019
Outros Autores: Marques,George L., Reis,Nadabe S., Maldonado,Rafael R., Santos,Rodrigo Luis S. R., Aguiar-Oliveira,Elizama
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000401393
Resumo: Abstract β-Cyclodextrin (β-CD) is a compound of great application for pharmaceutical and food industries, and is generally produced by starchy substrates by cyclomaltodextrin glucanotransferase (CGTase) action. The objective of this study was to produce β-CD using an alternative source of starch, such as jackfruit seed (Artocarpus intergrifolia L.) bran (JSB) by a commercial CGTase. The highest productivity of β-CD (52.10 µM/h.g) was obtained from 10 g of JSB in 100 mL of citrate buffer (10 mM / pH 6.0), with 17 % (v/v) of ethanol and 1.34 U/g of CGTase, at 59 °C for 4 hours. These same conditions were applied to starches extracted from the JSB (SJSB) and ginger (SG) and also, potato starch (SP). The SJSB and SG performances were similar to SP, and resulted in productivities around 2.7 times higher in relation to JSB. Thus, it is possible to conclude that both JSB and SJSB are promising substrates for β-CD production.
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spelling ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)Cyclomaltodextrin glucanotransferaseFactorial designGingerPhenolphthaleinStarchAbstract β-Cyclodextrin (β-CD) is a compound of great application for pharmaceutical and food industries, and is generally produced by starchy substrates by cyclomaltodextrin glucanotransferase (CGTase) action. The objective of this study was to produce β-CD using an alternative source of starch, such as jackfruit seed (Artocarpus intergrifolia L.) bran (JSB) by a commercial CGTase. The highest productivity of β-CD (52.10 µM/h.g) was obtained from 10 g of JSB in 100 mL of citrate buffer (10 mM / pH 6.0), with 17 % (v/v) of ethanol and 1.34 U/g of CGTase, at 59 °C for 4 hours. These same conditions were applied to starches extracted from the JSB (SJSB) and ginger (SG) and also, potato starch (SP). The SJSB and SG performances were similar to SP, and resulted in productivities around 2.7 times higher in relation to JSB. Thus, it is possible to conclude that both JSB and SJSB are promising substrates for β-CD production.Brazilian Society of Chemical Engineering2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000401393Brazilian Journal of Chemical Engineering v.36 n.4 2019reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20190364s20180343info:eu-repo/semantics/openAccessSilva Júnior,Benesval L.Marques,George L.Reis,Nadabe S.Maldonado,Rafael R.Santos,Rodrigo Luis S. R.Aguiar-Oliveira,Elizamaeng2020-01-08T00:00:00Zoai:scielo:S0104-66322019000401393Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2020-01-08T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
title ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
spellingShingle ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
Silva Júnior,Benesval L.
Cyclomaltodextrin glucanotransferase
Factorial design
Ginger
Phenolphthalein
Starch
title_short ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
title_full ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
title_fullStr ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
title_full_unstemmed ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
title_sort ENZYMATIC PRODUCTION OF β-CYCLODEXTRIN FROM JACKFRUIT SEEDS (Artocarpus intergrifolia L.)
author Silva Júnior,Benesval L.
author_facet Silva Júnior,Benesval L.
Marques,George L.
Reis,Nadabe S.
Maldonado,Rafael R.
Santos,Rodrigo Luis S. R.
Aguiar-Oliveira,Elizama
author_role author
author2 Marques,George L.
Reis,Nadabe S.
Maldonado,Rafael R.
Santos,Rodrigo Luis S. R.
Aguiar-Oliveira,Elizama
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva Júnior,Benesval L.
Marques,George L.
Reis,Nadabe S.
Maldonado,Rafael R.
Santos,Rodrigo Luis S. R.
Aguiar-Oliveira,Elizama
dc.subject.por.fl_str_mv Cyclomaltodextrin glucanotransferase
Factorial design
Ginger
Phenolphthalein
Starch
topic Cyclomaltodextrin glucanotransferase
Factorial design
Ginger
Phenolphthalein
Starch
description Abstract β-Cyclodextrin (β-CD) is a compound of great application for pharmaceutical and food industries, and is generally produced by starchy substrates by cyclomaltodextrin glucanotransferase (CGTase) action. The objective of this study was to produce β-CD using an alternative source of starch, such as jackfruit seed (Artocarpus intergrifolia L.) bran (JSB) by a commercial CGTase. The highest productivity of β-CD (52.10 µM/h.g) was obtained from 10 g of JSB in 100 mL of citrate buffer (10 mM / pH 6.0), with 17 % (v/v) of ethanol and 1.34 U/g of CGTase, at 59 °C for 4 hours. These same conditions were applied to starches extracted from the JSB (SJSB) and ginger (SG) and also, potato starch (SP). The SJSB and SG performances were similar to SP, and resulted in productivities around 2.7 times higher in relation to JSB. Thus, it is possible to conclude that both JSB and SJSB are promising substrates for β-CD production.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000401393
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000401393
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0104-6632.20190364s20180343
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.36 n.4 2019
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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