Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography

Detalhes bibliográficos
Autor(a) principal: MINAMI,N.M.
Data de Publicação: 1999
Outros Autores: LUCARINI,A.C., KILIKIAN,B.V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012
Resumo: In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa.
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spelling Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatographyGlucoamylasegel filtration chromatographypurificationsubmerse culturesemisolid cultureIn this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa.Brazilian Society of Chemical Engineering1999-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012Brazilian Journal of Chemical Engineering v.16 n.2 1999reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66321999000200012info:eu-repo/semantics/openAccessMINAMI,N.M.LUCARINI,A.C.KILIKIAN,B.V.eng1999-09-15T00:00:00Zoai:scielo:S0104-66321999000200012Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:1999-09-15T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
title Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
spellingShingle Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
MINAMI,N.M.
Glucoamylase
gel filtration chromatography
purification
submerse culture
semisolid culture
title_short Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
title_full Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
title_fullStr Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
title_full_unstemmed Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
title_sort Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography
author MINAMI,N.M.
author_facet MINAMI,N.M.
LUCARINI,A.C.
KILIKIAN,B.V.
author_role author
author2 LUCARINI,A.C.
KILIKIAN,B.V.
author2_role author
author
dc.contributor.author.fl_str_mv MINAMI,N.M.
LUCARINI,A.C.
KILIKIAN,B.V.
dc.subject.por.fl_str_mv Glucoamylase
gel filtration chromatography
purification
submerse culture
semisolid culture
topic Glucoamylase
gel filtration chromatography
purification
submerse culture
semisolid culture
description In this study, a preparative size-exclusion chromatography of two different clarified media obtained from submerse and semisolid culture of the mold Aspergillus awamori was carried out. Characterization and comparison of the quantities of glucoamylase and contaminant proteins present in these media were possible. Glucoamylase is the protein with the higher molecular weight in both media analyzed, varying from 72 to 80kDa in the submerse culture and from 68 to 90kDa in the semisolid culture. Also, glucoamylase protein concentration is higher in the submerse culture than in the semisolid culture. The other proteins in the submerse culture presented molecular weights lower than 12kDa and in the semisolid culture their molecular weights varied from 21 to 37kDa and below 10kDa.
publishDate 1999
dc.date.none.fl_str_mv 1999-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321999000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66321999000200012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.16 n.2 1999
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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