SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION

Detalhes bibliográficos
Autor(a) principal: Cintra,F. de O.
Data de Publicação: 2018
Outros Autores: Takagi,M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000401305
Resumo: ABSTRACT Haemophilus influenzae type b (Hib) vaccine is made up from its capsular polysaccharide (PRP). Low productivity of the polysaccharide during cell growth increases the final cost of this vaccine. Hib achieves low levels of cellular concentration in vitro due to the inhibition caused by acetate. The Akaike Information Criterion (AIC) was used in this work for selecting models of microbial growth. The application to the case of the multivariate models is outlined and the procedure is carried out using data from Hib cultures. From 4 models of biomass and 15 of acetate and PRP, one could be selected with great evidence for support. The use of AIC has shown to be robust and free of subjectivity, and it was able to define a kinetic model that is adequate for the cell growth and the production of its PRP over a wide range of culture conditions. The exponential inhibition factor was found to be the best for modelling inhibition of cell growth by acetate, while the hyperbolic factor was the best for inhibition of PRP formation. The acetate formation was found to have both growth associated and non-associated types. PRP formation was found to be only growth associated.
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spelling SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERIONHaemophilus influenzae type bAkaike’s Information CriterionMathematical modelBiomassPolysaccharideABSTRACT Haemophilus influenzae type b (Hib) vaccine is made up from its capsular polysaccharide (PRP). Low productivity of the polysaccharide during cell growth increases the final cost of this vaccine. Hib achieves low levels of cellular concentration in vitro due to the inhibition caused by acetate. The Akaike Information Criterion (AIC) was used in this work for selecting models of microbial growth. The application to the case of the multivariate models is outlined and the procedure is carried out using data from Hib cultures. From 4 models of biomass and 15 of acetate and PRP, one could be selected with great evidence for support. The use of AIC has shown to be robust and free of subjectivity, and it was able to define a kinetic model that is adequate for the cell growth and the production of its PRP over a wide range of culture conditions. The exponential inhibition factor was found to be the best for modelling inhibition of cell growth by acetate, while the hyperbolic factor was the best for inhibition of PRP formation. The acetate formation was found to have both growth associated and non-associated types. PRP formation was found to be only growth associated.Brazilian Society of Chemical Engineering2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000401305Brazilian Journal of Chemical Engineering v.35 n.4 2018reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20180354s20170085info:eu-repo/semantics/openAccessCintra,F. de O.Takagi,M.eng2019-03-20T00:00:00Zoai:scielo:S0104-66322018000401305Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2019-03-20T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
title SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
spellingShingle SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
Cintra,F. de O.
Haemophilus influenzae type b
Akaike’s Information Criterion
Mathematical model
Biomass
Polysaccharide
title_short SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
title_full SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
title_fullStr SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
title_full_unstemmed SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
title_sort SELECTION OF A MATHEMATICAL MODEL FOR THE KINETICS OF Haemophilus influenzae TYPE B USING AKAIKE’S INFORMATION CRITERION
author Cintra,F. de O.
author_facet Cintra,F. de O.
Takagi,M.
author_role author
author2 Takagi,M.
author2_role author
dc.contributor.author.fl_str_mv Cintra,F. de O.
Takagi,M.
dc.subject.por.fl_str_mv Haemophilus influenzae type b
Akaike’s Information Criterion
Mathematical model
Biomass
Polysaccharide
topic Haemophilus influenzae type b
Akaike’s Information Criterion
Mathematical model
Biomass
Polysaccharide
description ABSTRACT Haemophilus influenzae type b (Hib) vaccine is made up from its capsular polysaccharide (PRP). Low productivity of the polysaccharide during cell growth increases the final cost of this vaccine. Hib achieves low levels of cellular concentration in vitro due to the inhibition caused by acetate. The Akaike Information Criterion (AIC) was used in this work for selecting models of microbial growth. The application to the case of the multivariate models is outlined and the procedure is carried out using data from Hib cultures. From 4 models of biomass and 15 of acetate and PRP, one could be selected with great evidence for support. The use of AIC has shown to be robust and free of subjectivity, and it was able to define a kinetic model that is adequate for the cell growth and the production of its PRP over a wide range of culture conditions. The exponential inhibition factor was found to be the best for modelling inhibition of cell growth by acetate, while the hyperbolic factor was the best for inhibition of PRP formation. The acetate formation was found to have both growth associated and non-associated types. PRP formation was found to be only growth associated.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000401305
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322018000401305
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0104-6632.20180354s20170085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.35 n.4 2018
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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