The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322001000200005 |
Resumo: | With the aim of producing polyhydroxyalkanoates, a study of the influence of culture conditions (nitrogen and carbon sources and temperature) on the growth of Ralstonia eutropha in stirred flasks was carried out and the use of some low-cost sources (hydrolyzed lactose, inverted sugar and corn steep liquor) as evaluated. The best specific growth rate was obtained when inverted sugar was utilized as the substrate (mumax = 0.26 h-1). Two different phases in the assimilation of the carbon source were observed when hydrolyzed lactose was present, suggesting the assimilation first of glucose and then of galactose. To confirm the growth of Ralstonia eutropha using galactose as the only carbon source, experiments were carried out and the results showed that this bacterium is able to grow in the presence of this sugar at a growth rate of 0.13 h-1. The use of galactose by Ralstonia eutropha for its growth has not been reported in the literature until now. Corn steep liquor was found to be a viable alternative nitrogen source to ammonium sulfate. The results of experiments carried out at 30°C and 34°C were similar. |
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Brazilian Journal of Chemical Engineering |
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The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoatecorn steep liquorinverted sugarpolyhydroxyalkanoatesRalstonia eutrophaspecific growth rateWith the aim of producing polyhydroxyalkanoates, a study of the influence of culture conditions (nitrogen and carbon sources and temperature) on the growth of Ralstonia eutropha in stirred flasks was carried out and the use of some low-cost sources (hydrolyzed lactose, inverted sugar and corn steep liquor) as evaluated. The best specific growth rate was obtained when inverted sugar was utilized as the substrate (mumax = 0.26 h-1). Two different phases in the assimilation of the carbon source were observed when hydrolyzed lactose was present, suggesting the assimilation first of glucose and then of galactose. To confirm the growth of Ralstonia eutropha using galactose as the only carbon source, experiments were carried out and the results showed that this bacterium is able to grow in the presence of this sugar at a growth rate of 0.13 h-1. The use of galactose by Ralstonia eutropha for its growth has not been reported in the literature until now. Corn steep liquor was found to be a viable alternative nitrogen source to ammonium sulfate. The results of experiments carried out at 30°C and 34°C were similar.Brazilian Society of Chemical Engineering2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322001000200005Brazilian Journal of Chemical Engineering v.18 n.2 2001reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322001000200005info:eu-repo/semantics/openAccessMarangoni,C.Furigo Jr.,A.Aragão,G.M.F.eng2001-08-06T00:00:00Zoai:scielo:S0104-66322001000200005Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2001-08-06T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
title |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
spellingShingle |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate Marangoni,C. corn steep liquor inverted sugar polyhydroxyalkanoates Ralstonia eutropha specific growth rate |
title_short |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
title_full |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
title_fullStr |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
title_full_unstemmed |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
title_sort |
The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate |
author |
Marangoni,C. |
author_facet |
Marangoni,C. Furigo Jr.,A. Aragão,G.M.F. |
author_role |
author |
author2 |
Furigo Jr.,A. Aragão,G.M.F. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Marangoni,C. Furigo Jr.,A. Aragão,G.M.F. |
dc.subject.por.fl_str_mv |
corn steep liquor inverted sugar polyhydroxyalkanoates Ralstonia eutropha specific growth rate |
topic |
corn steep liquor inverted sugar polyhydroxyalkanoates Ralstonia eutropha specific growth rate |
description |
With the aim of producing polyhydroxyalkanoates, a study of the influence of culture conditions (nitrogen and carbon sources and temperature) on the growth of Ralstonia eutropha in stirred flasks was carried out and the use of some low-cost sources (hydrolyzed lactose, inverted sugar and corn steep liquor) as evaluated. The best specific growth rate was obtained when inverted sugar was utilized as the substrate (mumax = 0.26 h-1). Two different phases in the assimilation of the carbon source were observed when hydrolyzed lactose was present, suggesting the assimilation first of glucose and then of galactose. To confirm the growth of Ralstonia eutropha using galactose as the only carbon source, experiments were carried out and the results showed that this bacterium is able to grow in the presence of this sugar at a growth rate of 0.13 h-1. The use of galactose by Ralstonia eutropha for its growth has not been reported in the literature until now. Corn steep liquor was found to be a viable alternative nitrogen source to ammonium sulfate. The results of experiments carried out at 30°C and 34°C were similar. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322001000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322001000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322001000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.18 n.2 2001 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213171082559488 |