Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322011000400003 |
Resumo: | Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up. |
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Brazilian Journal of Chemical Engineering |
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Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltrationFlagellinPurificationTangential ultrafiltrationSalmonella TyphimuriumFlagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up.Brazilian Society of Chemical Engineering2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322011000400003Brazilian Journal of Chemical Engineering v.28 n.4 2011reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322011000400003info:eu-repo/semantics/openAccessOliveira,B. H.Silva,M. R.Braga,C. J. M.Massis,L. M.Ferreira,L. C. S.Sbrogio-Almeida,M. E.Takagi,M.eng2011-11-24T00:00:00Zoai:scielo:S0104-66322011000400003Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2011-11-24T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
title |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
spellingShingle |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration Oliveira,B. H. Flagellin Purification Tangential ultrafiltration Salmonella Typhimurium |
title_short |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
title_full |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
title_fullStr |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
title_full_unstemmed |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
title_sort |
Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration |
author |
Oliveira,B. H. |
author_facet |
Oliveira,B. H. Silva,M. R. Braga,C. J. M. Massis,L. M. Ferreira,L. C. S. Sbrogio-Almeida,M. E. Takagi,M. |
author_role |
author |
author2 |
Silva,M. R. Braga,C. J. M. Massis,L. M. Ferreira,L. C. S. Sbrogio-Almeida,M. E. Takagi,M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,B. H. Silva,M. R. Braga,C. J. M. Massis,L. M. Ferreira,L. C. S. Sbrogio-Almeida,M. E. Takagi,M. |
dc.subject.por.fl_str_mv |
Flagellin Purification Tangential ultrafiltration Salmonella Typhimurium |
topic |
Flagellin Purification Tangential ultrafiltration Salmonella Typhimurium |
description |
Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322011000400003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322011000400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322011000400003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.28 n.4 2011 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173497430016 |