Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000300001 |
Resumo: | Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate. |
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Brazilian Journal of Chemical Engineering |
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Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10cBatch cultureContinuous culturePenicillium canescensXylanasePenicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate.Brazilian Society of Chemical Engineering2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000300001Brazilian Journal of Chemical Engineering v.29 n.3 2012reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322012000300001info:eu-repo/semantics/openAccessBakri,Y.Akeed,Y.Thonart,P.eng2012-10-25T00:00:00Zoai:scielo:S0104-66322012000300001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2012-10-25T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
title |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
spellingShingle |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c Bakri,Y. Batch culture Continuous culture Penicillium canescens Xylanase |
title_short |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
title_full |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
title_fullStr |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
title_full_unstemmed |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
title_sort |
Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c |
author |
Bakri,Y. |
author_facet |
Bakri,Y. Akeed,Y. Thonart,P. |
author_role |
author |
author2 |
Akeed,Y. Thonart,P. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bakri,Y. Akeed,Y. Thonart,P. |
dc.subject.por.fl_str_mv |
Batch culture Continuous culture Penicillium canescens Xylanase |
topic |
Batch culture Continuous culture Penicillium canescens Xylanase |
description |
Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322012000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322012000300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.29 n.3 2012 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173819342848 |