Kinetic study of the enzymatic hydrolysis of sugarcane bagasse

• Main Author: Carvalho,M. L.
• Publication Date: 2013
• Other Authors: Sousa Jr.,R., Rodríguez-Zúñiga,U. F., Suarez,C. A. G., Rodrigues,D. S., Giordano,R. C., Giordano,R. L. C.
• Format: Article
• Language: eng
• Source: Brazilian Journal of Chemical Engineering
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000300002
• topic: Cellulose enzymatic hydrolysis; Sugarcane bagasse; Kinetic study

This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a low recalcitrance substrate model; steam exploded sugarcane bagasse (SB); and weak acid pretreated SB (1:20 dry bagasse:H2SO4 solution 1% w/w), the last two delignified with 4% NaOH (w/w). The influence of substrate concentration was assessed in hydrolysis experiments in a shaker, using Accellerase® 1500, at pH 4.8, in 50 mM sodium citrate buffer. Cellulose loads (weight substrate/weight total) were changed between 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP and low loads of steam exploded SB, it was possible to fit pseudo-homogeneous Michaelis-Menten models (with inhibition). For FP and higher loads of steam exploded SB, modified Michaelis-Menten models were fitted. Besides, it was observed that, after retuning of the model parameters, it is possible to apply a model fitted for one situation to a different case. Chrastil models were also fitted and they were the only feasible approach for the highly recalcitrant acid-treated SB.