Development of a bioprocess for the production of actinomycin-D
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322002000300002 |
Resumo: | S. parvulus was selected from three species of Streptomyces that produce actinomycins, as it has the greatest antibiotic activity (152 mg/L) and produces only actinomycin-D. Aiming at improving the antibiotic production in shaken flasks, the substitution of glucose by fructose in concentrations of 20, 30 and 40 g/L was investigated. In all cases this replacement led to an increase in the antibiotic production, reaching a maximum concentration of 635 mg/L for an initial fructose concentration of 30 g/L. In experiments conducted in a bioreactor at different degrees of aeration (0.5, 1.0 and 1.5 vvm) and stirring speeds (300 and 500 rpm), it was found that the greatest antibiotic production (1530 mg/L) occurred with a aeration of 1.5 vvm and a stirring speed of 500 rpm. The fermented medium showed the rheological behavior of a pseudoplastic fluid. |
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Brazilian Journal of Chemical Engineering |
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Development of a bioprocess for the production of actinomycin-Dactinomycin-DS. parvulusS. felleusS. regensisfermentationrheologyS. parvulus was selected from three species of Streptomyces that produce actinomycins, as it has the greatest antibiotic activity (152 mg/L) and produces only actinomycin-D. Aiming at improving the antibiotic production in shaken flasks, the substitution of glucose by fructose in concentrations of 20, 30 and 40 g/L was investigated. In all cases this replacement led to an increase in the antibiotic production, reaching a maximum concentration of 635 mg/L for an initial fructose concentration of 30 g/L. In experiments conducted in a bioreactor at different degrees of aeration (0.5, 1.0 and 1.5 vvm) and stirring speeds (300 and 500 rpm), it was found that the greatest antibiotic production (1530 mg/L) occurred with a aeration of 1.5 vvm and a stirring speed of 500 rpm. The fermented medium showed the rheological behavior of a pseudoplastic fluid.Brazilian Society of Chemical Engineering2002-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322002000300002Brazilian Journal of Chemical Engineering v.19 n.3 2002reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322002000300002info:eu-repo/semantics/openAccessSousa,M.F.V.Q.Lopes,C.E.Pereira Jr.,N.eng2003-01-21T00:00:00Zoai:scielo:S0104-66322002000300002Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2003-01-21T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Development of a bioprocess for the production of actinomycin-D |
title |
Development of a bioprocess for the production of actinomycin-D |
spellingShingle |
Development of a bioprocess for the production of actinomycin-D Sousa,M.F.V.Q. actinomycin-D S. parvulus S. felleus S. regensis fermentation rheology |
title_short |
Development of a bioprocess for the production of actinomycin-D |
title_full |
Development of a bioprocess for the production of actinomycin-D |
title_fullStr |
Development of a bioprocess for the production of actinomycin-D |
title_full_unstemmed |
Development of a bioprocess for the production of actinomycin-D |
title_sort |
Development of a bioprocess for the production of actinomycin-D |
author |
Sousa,M.F.V.Q. |
author_facet |
Sousa,M.F.V.Q. Lopes,C.E. Pereira Jr.,N. |
author_role |
author |
author2 |
Lopes,C.E. Pereira Jr.,N. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Sousa,M.F.V.Q. Lopes,C.E. Pereira Jr.,N. |
dc.subject.por.fl_str_mv |
actinomycin-D S. parvulus S. felleus S. regensis fermentation rheology |
topic |
actinomycin-D S. parvulus S. felleus S. regensis fermentation rheology |
description |
S. parvulus was selected from three species of Streptomyces that produce actinomycins, as it has the greatest antibiotic activity (152 mg/L) and produces only actinomycin-D. Aiming at improving the antibiotic production in shaken flasks, the substitution of glucose by fructose in concentrations of 20, 30 and 40 g/L was investigated. In all cases this replacement led to an increase in the antibiotic production, reaching a maximum concentration of 635 mg/L for an initial fructose concentration of 30 g/L. In experiments conducted in a bioreactor at different degrees of aeration (0.5, 1.0 and 1.5 vvm) and stirring speeds (300 and 500 rpm), it was found that the greatest antibiotic production (1530 mg/L) occurred with a aeration of 1.5 vvm and a stirring speed of 500 rpm. The fermented medium showed the rheological behavior of a pseudoplastic fluid. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322002000300002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322002000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322002000300002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.19 n.3 2002 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213171152814080 |