Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops

Detalhes bibliográficos
Autor(a) principal: Barros,Aline F
Data de Publicação: 2018
Outros Autores: Campos,Vicente P, Souza,Larissa N, Costa,Sarah S, Terra,Willian C, Lessa,Josimar HL
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Horticultura Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473
Resumo: ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent. Here, the species M. arenaria parasitizing scarlet eggplant and M. morocciensis parasitizing pumpkin and cabbage are reported in Brazil for the first time. Esterase electrophoresis efficiently separated the species of Meloidogyne found in vegetable crops; however, SCAR markers were only effective for the identification of M. incognita, M. javanica, and M. hapla, since the primer pair Far/Rar yielded no amplification product to confirm the identity of M. arenaria. The species M.arenaria and M. morocciensis could not be distinguished by the female perineal patterns. Based on the present results, new primers should be designed for the identification of M. arenaria and M. morocciensis.
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spelling Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable cropsMeloidogynediagnosismolecular biologyvegetable cropsABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent. Here, the species M. arenaria parasitizing scarlet eggplant and M. morocciensis parasitizing pumpkin and cabbage are reported in Brazil for the first time. Esterase electrophoresis efficiently separated the species of Meloidogyne found in vegetable crops; however, SCAR markers were only effective for the identification of M. incognita, M. javanica, and M. hapla, since the primer pair Far/Rar yielded no amplification product to confirm the identity of M. arenaria. The species M.arenaria and M. morocciensis could not be distinguished by the female perineal patterns. Based on the present results, new primers should be designed for the identification of M. arenaria and M. morocciensis.Associação Brasileira de Horticultura2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473Horticultura Brasileira v.36 n.4 2018reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/s0102-053620180408info:eu-repo/semantics/openAccessBarros,Aline FCampos,Vicente PSouza,Larissa NCosta,Sarah STerra,Willian CLessa,Josimar HLeng2018-12-17T00:00:00Zoai:scielo:S0102-05362018000400473Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2018-12-17T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false
dc.title.none.fl_str_mv Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
title Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
spellingShingle Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
Barros,Aline F
Meloidogyne
diagnosis
molecular biology
vegetable crops
title_short Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
title_full Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
title_fullStr Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
title_full_unstemmed Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
title_sort Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
author Barros,Aline F
author_facet Barros,Aline F
Campos,Vicente P
Souza,Larissa N
Costa,Sarah S
Terra,Willian C
Lessa,Josimar HL
author_role author
author2 Campos,Vicente P
Souza,Larissa N
Costa,Sarah S
Terra,Willian C
Lessa,Josimar HL
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barros,Aline F
Campos,Vicente P
Souza,Larissa N
Costa,Sarah S
Terra,Willian C
Lessa,Josimar HL
dc.subject.por.fl_str_mv Meloidogyne
diagnosis
molecular biology
vegetable crops
topic Meloidogyne
diagnosis
molecular biology
vegetable crops
description ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent. Here, the species M. arenaria parasitizing scarlet eggplant and M. morocciensis parasitizing pumpkin and cabbage are reported in Brazil for the first time. Esterase electrophoresis efficiently separated the species of Meloidogyne found in vegetable crops; however, SCAR markers were only effective for the identification of M. incognita, M. javanica, and M. hapla, since the primer pair Far/Rar yielded no amplification product to confirm the identity of M. arenaria. The species M.arenaria and M. morocciensis could not be distinguished by the female perineal patterns. Based on the present results, new primers should be designed for the identification of M. arenaria and M. morocciensis.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-053620180408
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Horticultura
publisher.none.fl_str_mv Associação Brasileira de Horticultura
dc.source.none.fl_str_mv Horticultura Brasileira v.36 n.4 2018
reponame:Horticultura Brasileira
instname:Associação Brasileira de Horticultura (ABH)
instacron:ABH
instname_str Associação Brasileira de Horticultura (ABH)
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institution ABH
reponame_str Horticultura Brasileira
collection Horticultura Brasileira
repository.name.fl_str_mv Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)
repository.mail.fl_str_mv ||hortbras@gmail.com
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