In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations

• Autor(a) principal: Faria,Ricardo T. de
• Data de Publicação: 2004
• Outros Autores: Rodrigues,Fabiana N., Oliveira,Luciana do V.R., Müller,Cláudio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Horticultura Brasileira
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362004000400023
• topic: Dendrobium nobile; Orchidaceae; carbohydrate; tissue culture

Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.