Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Horticultura Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019 |
Resumo: | Disinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely. |
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Horticultura Brasileira |
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Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazilorchidsin vitro culturegerminationsodium hypocloriteformaldehydeDisinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely.Associação Brasileira de Horticultura2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019Horticultura Brasileira v.24 n.2 2006reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/S0102-05362006000200019info:eu-repo/semantics/openAccessAlvarez-Pardo,Veronica MFerreira,Alfredo GuiNunes,Valter Feng2007-05-28T00:00:00Zoai:scielo:S0102-05362006000200019Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2007-05-28T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false |
dc.title.none.fl_str_mv |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
title |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
spellingShingle |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil Alvarez-Pardo,Veronica M orchids in vitro culture germination sodium hypoclorite formaldehyde |
title_short |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
title_full |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
title_fullStr |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
title_full_unstemmed |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
title_sort |
Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil |
author |
Alvarez-Pardo,Veronica M |
author_facet |
Alvarez-Pardo,Veronica M Ferreira,Alfredo Gui Nunes,Valter F |
author_role |
author |
author2 |
Ferreira,Alfredo Gui Nunes,Valter F |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Alvarez-Pardo,Veronica M Ferreira,Alfredo Gui Nunes,Valter F |
dc.subject.por.fl_str_mv |
orchids in vitro culture germination sodium hypoclorite formaldehyde |
topic |
orchids in vitro culture germination sodium hypoclorite formaldehyde |
description |
Disinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-05362006000200019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
dc.source.none.fl_str_mv |
Horticultura Brasileira v.24 n.2 2006 reponame:Horticultura Brasileira instname:Associação Brasileira de Horticultura (ABH) instacron:ABH |
instname_str |
Associação Brasileira de Horticultura (ABH) |
instacron_str |
ABH |
institution |
ABH |
reponame_str |
Horticultura Brasileira |
collection |
Horticultura Brasileira |
repository.name.fl_str_mv |
Horticultura Brasileira - Associação Brasileira de Horticultura (ABH) |
repository.mail.fl_str_mv |
||hortbras@gmail.com |
_version_ |
1754213078998712320 |