In vitro propagation of five Alocasia species
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Horticultura Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006 |
Resumo: | The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot growth and proliferation for all the tested species. For A. amazonica, A. cuprea, A. robusta and A. longiloba, shake flask system using liquid medium of the same constituents stimulated more shoot proliferation as compared to agar-gelled medium. However, for A. chaii there was no significant difference. All the in vitro plantlets with well developed roots and leaves were successfully acclimatized with more than 90% survival rate. |
id |
ABH-1_d3599419e50a530b8bc35d95ef5710de |
---|---|
oai_identifier_str |
oai:scielo:S0102-05362013000200006 |
network_acronym_str |
ABH-1 |
network_name_str |
Horticultura Brasileira |
repository_id_str |
|
spelling |
In vitro propagation of five Alocasia speciesAlocasia amazonicaA. cupreaA. robustaA. longilobaA. chaiiculture mediumacclimatizationThe influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot growth and proliferation for all the tested species. For A. amazonica, A. cuprea, A. robusta and A. longiloba, shake flask system using liquid medium of the same constituents stimulated more shoot proliferation as compared to agar-gelled medium. However, for A. chaii there was no significant difference. All the in vitro plantlets with well developed roots and leaves were successfully acclimatized with more than 90% survival rate.Associação Brasileira de Horticultura2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006Horticultura Brasileira v.31 n.2 2013reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/S0102-05362013000200006info:eu-repo/semantics/openAccessBhatt,ArvindStanly,ChristineKeng,Chan Laieng2013-07-18T00:00:00Zoai:scielo:S0102-05362013000200006Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2013-07-18T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false |
dc.title.none.fl_str_mv |
In vitro propagation of five Alocasia species |
title |
In vitro propagation of five Alocasia species |
spellingShingle |
In vitro propagation of five Alocasia species Bhatt,Arvind Alocasia amazonica A. cuprea A. robusta A. longiloba A. chaii culture medium acclimatization |
title_short |
In vitro propagation of five Alocasia species |
title_full |
In vitro propagation of five Alocasia species |
title_fullStr |
In vitro propagation of five Alocasia species |
title_full_unstemmed |
In vitro propagation of five Alocasia species |
title_sort |
In vitro propagation of five Alocasia species |
author |
Bhatt,Arvind |
author_facet |
Bhatt,Arvind Stanly,Christine Keng,Chan Lai |
author_role |
author |
author2 |
Stanly,Christine Keng,Chan Lai |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bhatt,Arvind Stanly,Christine Keng,Chan Lai |
dc.subject.por.fl_str_mv |
Alocasia amazonica A. cuprea A. robusta A. longiloba A. chaii culture medium acclimatization |
topic |
Alocasia amazonica A. cuprea A. robusta A. longiloba A. chaii culture medium acclimatization |
description |
The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot growth and proliferation for all the tested species. For A. amazonica, A. cuprea, A. robusta and A. longiloba, shake flask system using liquid medium of the same constituents stimulated more shoot proliferation as compared to agar-gelled medium. However, for A. chaii there was no significant difference. All the in vitro plantlets with well developed roots and leaves were successfully acclimatized with more than 90% survival rate. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-05362013000200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
dc.source.none.fl_str_mv |
Horticultura Brasileira v.31 n.2 2013 reponame:Horticultura Brasileira instname:Associação Brasileira de Horticultura (ABH) instacron:ABH |
instname_str |
Associação Brasileira de Horticultura (ABH) |
instacron_str |
ABH |
institution |
ABH |
reponame_str |
Horticultura Brasileira |
collection |
Horticultura Brasileira |
repository.name.fl_str_mv |
Horticultura Brasileira - Associação Brasileira de Horticultura (ABH) |
repository.mail.fl_str_mv |
||hortbras@gmail.com |
_version_ |
1754213081735495680 |