Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia

Detalhes bibliográficos
Autor(a) principal: Pierrot-Gallo,Bruna Spinella
Data de Publicação: 2015
Outros Autores: Vicari,Perla, Matsuda,Sandra Satiko, Adegoke,Samuel Ademola, Mecabo,Grazielle, Figueiredo,Maria Stella
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de hematologia e hemoterapia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500329
Resumo: BACKGROUND: Haptoglobin genotypes, and interleukin-6 and -8 participate in the pathophysiology of sickle cell anemia. The expression of cytokines is regulated by genetic mechanisms however the effect of haptoglobin polymorphisms on these cytokines is not fully understood. This study aimed to compare the frequency of haptoglobin genotypes and the interleukin-6 and -8 concentrations in sickle cell anemia patients and controls to investigate the association between haptoglobin genotypes and cytokine levels.METHODS: Sixty sickle cell anemia patients and 74 healthy individuals were analyzed. Haptoglobin genotypes were determined by multiplex polymerase chain reaction, and the interleukin-6 and -8 levels by enzyme linked immunosorbent assay. The association between haptoglobin genotypes and cytokines was investigated by statistical tests.RESULTS:Hp2-1 was the most common genotype in both the cases and controls while Hp1-1 was less frequent among sickle cell anemia patients. Interleukin-6 and -8 levels were higher in patients than controls (p-value <0.0001). There was no significant difference in interleukin-6 and -8 concentrations between the genotypes (p-value >0.05). A similar trend was observed among the controls.CONCLUSION: Although, levels of interleukin-6 and -8 were higher in the sickle cell anemia patients, they appeared not to be related to the haptoglobin genotypes. Further investigations are necessary to identify factors responsible for increased secretion of the interleukin-6 and -8 pro-inflammatory cytokines in patients with sickle cell anemia.
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spelling Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemiaHaptoglobin polymorphismGenotypeInterleukinsSickle cell anemiaBACKGROUND: Haptoglobin genotypes, and interleukin-6 and -8 participate in the pathophysiology of sickle cell anemia. The expression of cytokines is regulated by genetic mechanisms however the effect of haptoglobin polymorphisms on these cytokines is not fully understood. This study aimed to compare the frequency of haptoglobin genotypes and the interleukin-6 and -8 concentrations in sickle cell anemia patients and controls to investigate the association between haptoglobin genotypes and cytokine levels.METHODS: Sixty sickle cell anemia patients and 74 healthy individuals were analyzed. Haptoglobin genotypes were determined by multiplex polymerase chain reaction, and the interleukin-6 and -8 levels by enzyme linked immunosorbent assay. The association between haptoglobin genotypes and cytokines was investigated by statistical tests.RESULTS:Hp2-1 was the most common genotype in both the cases and controls while Hp1-1 was less frequent among sickle cell anemia patients. Interleukin-6 and -8 levels were higher in patients than controls (p-value <0.0001). There was no significant difference in interleukin-6 and -8 concentrations between the genotypes (p-value >0.05). A similar trend was observed among the controls.CONCLUSION: Although, levels of interleukin-6 and -8 were higher in the sickle cell anemia patients, they appeared not to be related to the haptoglobin genotypes. Further investigations are necessary to identify factors responsible for increased secretion of the interleukin-6 and -8 pro-inflammatory cytokines in patients with sickle cell anemia.Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500329Revista Brasileira de Hematologia e Hemoterapia v.37 n.5 2015reponame:Revista brasileira de hematologia e hemoterapia (Online)instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)instacron:ABHHTC10.1016/j.bjhh.2015.07.006info:eu-repo/semantics/openAccessPierrot-Gallo,Bruna SpinellaVicari,PerlaMatsuda,Sandra SatikoAdegoke,Samuel AdemolaMecabo,GrazielleFigueiredo,Maria Stellaeng2015-10-30T00:00:00Zoai:scielo:S1516-84842015000500329Revistahttp://www.rbhh.org/pt/archivo/https://old.scielo.br/oai/scielo-oai.phpsbhh@terra.com.br||secretaria@rbhh.org1806-08701516-8484opendoar:2015-10-30T00:00Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)false
dc.title.none.fl_str_mv Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
title Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
spellingShingle Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
Pierrot-Gallo,Bruna Spinella
Haptoglobin polymorphism
Genotype
Interleukins
Sickle cell anemia
title_short Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
title_full Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
title_fullStr Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
title_full_unstemmed Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
title_sort Haptoglobin gene polymorphisms and interleukin-6 and -8 levels in patients with sickle cell anemia
author Pierrot-Gallo,Bruna Spinella
author_facet Pierrot-Gallo,Bruna Spinella
Vicari,Perla
Matsuda,Sandra Satiko
Adegoke,Samuel Ademola
Mecabo,Grazielle
Figueiredo,Maria Stella
author_role author
author2 Vicari,Perla
Matsuda,Sandra Satiko
Adegoke,Samuel Ademola
Mecabo,Grazielle
Figueiredo,Maria Stella
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pierrot-Gallo,Bruna Spinella
Vicari,Perla
Matsuda,Sandra Satiko
Adegoke,Samuel Ademola
Mecabo,Grazielle
Figueiredo,Maria Stella
dc.subject.por.fl_str_mv Haptoglobin polymorphism
Genotype
Interleukins
Sickle cell anemia
topic Haptoglobin polymorphism
Genotype
Interleukins
Sickle cell anemia
description BACKGROUND: Haptoglobin genotypes, and interleukin-6 and -8 participate in the pathophysiology of sickle cell anemia. The expression of cytokines is regulated by genetic mechanisms however the effect of haptoglobin polymorphisms on these cytokines is not fully understood. This study aimed to compare the frequency of haptoglobin genotypes and the interleukin-6 and -8 concentrations in sickle cell anemia patients and controls to investigate the association between haptoglobin genotypes and cytokine levels.METHODS: Sixty sickle cell anemia patients and 74 healthy individuals were analyzed. Haptoglobin genotypes were determined by multiplex polymerase chain reaction, and the interleukin-6 and -8 levels by enzyme linked immunosorbent assay. The association between haptoglobin genotypes and cytokines was investigated by statistical tests.RESULTS:Hp2-1 was the most common genotype in both the cases and controls while Hp1-1 was less frequent among sickle cell anemia patients. Interleukin-6 and -8 levels were higher in patients than controls (p-value <0.0001). There was no significant difference in interleukin-6 and -8 concentrations between the genotypes (p-value >0.05). A similar trend was observed among the controls.CONCLUSION: Although, levels of interleukin-6 and -8 were higher in the sickle cell anemia patients, they appeared not to be related to the haptoglobin genotypes. Further investigations are necessary to identify factors responsible for increased secretion of the interleukin-6 and -8 pro-inflammatory cytokines in patients with sickle cell anemia.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500329
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500329
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjhh.2015.07.006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
dc.source.none.fl_str_mv Revista Brasileira de Hematologia e Hemoterapia v.37 n.5 2015
reponame:Revista brasileira de hematologia e hemoterapia (Online)
instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron:ABHHTC
instname_str Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron_str ABHHTC
institution ABHHTC
reponame_str Revista brasileira de hematologia e hemoterapia (Online)
collection Revista brasileira de hematologia e hemoterapia (Online)
repository.name.fl_str_mv Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
repository.mail.fl_str_mv sbhh@terra.com.br||secretaria@rbhh.org
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