A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Melo,Adriene Siqueira de
Data de Publicação: 2010
Outros Autores: Lorena,Virginia Maria Barros de, Braz,Suellen Carvalho de Moura, Gomes,Yara de Miranda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de hematologia e hemoterapia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000500012
Resumo: Venipuncture is one of the easiest clinical procedures to obtain viable blood samples to evaluate gene expression using mRNA analysis. However, the use of this sample type in reverse transcriptase polymerase chain reaction tests (RT-PCR) without prior treatment is controversial. We therefore propose to compare the suitability of different peripheral blood samples (whole blood without treatment, whole blood with hemolysis, peripheral blood mononuclear cells and frozen whole blood) for RT-PCR analysis. The results showed that, despite the blood sample being peripheral, it is possible to extract a fair amount of RNA and perform target gene amplification. Thus, peripheral blood without prior treatment could be used to investigate the gene expression using Real Time PCR.
id ABHHTC-1_e1ea8f37224bc9a0baf1ce2b7cccefa5
oai_identifier_str oai:scielo:S1516-84842010000500012
network_acronym_str ABHHTC-1
network_name_str Revista brasileira de hematologia e hemoterapia (Online)
repository_id_str
spelling A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reactionBlood specimen collection/methodsGene expressionRNA, messenger/bloodgenetic/immunologyReverse transcriptase polymerase chain reaction/Transcription, methodsSpectrophotometersVenipuncture is one of the easiest clinical procedures to obtain viable blood samples to evaluate gene expression using mRNA analysis. However, the use of this sample type in reverse transcriptase polymerase chain reaction tests (RT-PCR) without prior treatment is controversial. We therefore propose to compare the suitability of different peripheral blood samples (whole blood without treatment, whole blood with hemolysis, peripheral blood mononuclear cells and frozen whole blood) for RT-PCR analysis. The results showed that, despite the blood sample being peripheral, it is possible to extract a fair amount of RNA and perform target gene amplification. Thus, peripheral blood without prior treatment could be used to investigate the gene expression using Real Time PCR.Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000500012Revista Brasileira de Hematologia e Hemoterapia v.32 n.5 2010reponame:Revista brasileira de hematologia e hemoterapia (Online)instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)instacron:ABHHTC10.1590/S1516-84842010000500012info:eu-repo/semantics/openAccessMelo,Adriene Siqueira deLorena,Virginia Maria Barros deBraz,Suellen Carvalho de MouraGomes,Yara de Mirandaeng2012-02-01T00:00:00Zoai:scielo:S1516-84842010000500012Revistahttp://www.rbhh.org/pt/archivo/https://old.scielo.br/oai/scielo-oai.phpsbhh@terra.com.br||secretaria@rbhh.org1806-08701516-8484opendoar:2012-02-01T00:00Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)false
dc.title.none.fl_str_mv A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
title A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
spellingShingle A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
Melo,Adriene Siqueira de
Blood specimen collection/methods
Gene expression
RNA, messenger/blood
genetic/immunology
Reverse transcriptase polymerase chain reaction/Transcription, methods
Spectrophotometers
title_short A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
title_full A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
title_fullStr A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
title_full_unstemmed A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
title_sort A comparative analysis of the suitability of different peripheral blood samples for reverse transcriptase polymerase chain reaction
author Melo,Adriene Siqueira de
author_facet Melo,Adriene Siqueira de
Lorena,Virginia Maria Barros de
Braz,Suellen Carvalho de Moura
Gomes,Yara de Miranda
author_role author
author2 Lorena,Virginia Maria Barros de
Braz,Suellen Carvalho de Moura
Gomes,Yara de Miranda
author2_role author
author
author
dc.contributor.author.fl_str_mv Melo,Adriene Siqueira de
Lorena,Virginia Maria Barros de
Braz,Suellen Carvalho de Moura
Gomes,Yara de Miranda
dc.subject.por.fl_str_mv Blood specimen collection/methods
Gene expression
RNA, messenger/blood
genetic/immunology
Reverse transcriptase polymerase chain reaction/Transcription, methods
Spectrophotometers
topic Blood specimen collection/methods
Gene expression
RNA, messenger/blood
genetic/immunology
Reverse transcriptase polymerase chain reaction/Transcription, methods
Spectrophotometers
description Venipuncture is one of the easiest clinical procedures to obtain viable blood samples to evaluate gene expression using mRNA analysis. However, the use of this sample type in reverse transcriptase polymerase chain reaction tests (RT-PCR) without prior treatment is controversial. We therefore propose to compare the suitability of different peripheral blood samples (whole blood without treatment, whole blood with hemolysis, peripheral blood mononuclear cells and frozen whole blood) for RT-PCR analysis. The results showed that, despite the blood sample being peripheral, it is possible to extract a fair amount of RNA and perform target gene amplification. Thus, peripheral blood without prior treatment could be used to investigate the gene expression using Real Time PCR.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000500012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000500012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-84842010000500012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
dc.source.none.fl_str_mv Revista Brasileira de Hematologia e Hemoterapia v.32 n.5 2010
reponame:Revista brasileira de hematologia e hemoterapia (Online)
instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron:ABHHTC
instname_str Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron_str ABHHTC
institution ABHHTC
reponame_str Revista brasileira de hematologia e hemoterapia (Online)
collection Revista brasileira de hematologia e hemoterapia (Online)
repository.name.fl_str_mv Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
repository.mail.fl_str_mv sbhh@terra.com.br||secretaria@rbhh.org
_version_ 1754213110334357504

Registros relacionados