Screening of cyanotoxin producing genes in Ecuadorian freshwater systems

Detalhes bibliográficos
Autor(a) principal: Ballesteros,Isabel
Data de Publicação: 2022
Outros Autores: Cruz,Sara de la, Rojas,Michelle, Salazar,Génesis, Martínez-Fresneda,Miguel, Castillejo,Pablo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Limnologica Brasiliensia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720
Resumo: Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.
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spelling Screening of cyanotoxin producing genes in Ecuadorian freshwater systemscylindrospermopsinmicrocystinnested PCRAndesAmazonAbstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.Associação Brasileira de Limnologia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720Acta Limnologica Brasiliensia v.34 2022reponame:Acta Limnologica Brasiliensia (Online)instname:Associação Brasileira de Limnologia (ABL)instacron:ABL10.1590/s2179-975x2122info:eu-repo/semantics/openAccessBallesteros,IsabelCruz,Sara de laRojas,MichelleSalazar,GénesisMartínez-Fresneda,MiguelCastillejo,Pabloeng2022-10-11T00:00:00Zoai:scielo:S2179-975X2022000100720Revistahttp://www.ablimno.org.br/publiActa.phphttps://old.scielo.br/oai/scielo-oai.php||actalb@rc.unesp.br2179-975X0102-6712opendoar:2022-10-11T00:00Acta Limnologica Brasiliensia (Online) - Associação Brasileira de Limnologia (ABL)false
dc.title.none.fl_str_mv Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
spellingShingle Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
Ballesteros,Isabel
cylindrospermopsin
microcystin
nested PCR
Andes
Amazon
title_short Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_full Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_fullStr Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_full_unstemmed Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
title_sort Screening of cyanotoxin producing genes in Ecuadorian freshwater systems
author Ballesteros,Isabel
author_facet Ballesteros,Isabel
Cruz,Sara de la
Rojas,Michelle
Salazar,Génesis
Martínez-Fresneda,Miguel
Castillejo,Pablo
author_role author
author2 Cruz,Sara de la
Rojas,Michelle
Salazar,Génesis
Martínez-Fresneda,Miguel
Castillejo,Pablo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ballesteros,Isabel
Cruz,Sara de la
Rojas,Michelle
Salazar,Génesis
Martínez-Fresneda,Miguel
Castillejo,Pablo
dc.subject.por.fl_str_mv cylindrospermopsin
microcystin
nested PCR
Andes
Amazon
topic cylindrospermopsin
microcystin
nested PCR
Andes
Amazon
description Abstract: Aim This study pursued the detection of cyr and mcy genes to assess the presence of cylindrospemopsin (CN) and microcystin (MC) potential producers in Ecuadorian water bodies. Methods Environmental DNA (eDNA) was extracted from eight water bodies and one wastewater treatment plant (WWTP) from Ecuador. A nested PCR was designed to amplify mcyB, cyrE, and cyrJ genes in these environmental samples. PCR products were sequenced and blasted against GenBank database. Results Potential CN producers were found in seven water bodies and the WWTP. cyrE amplification revealed three variants belonging to Raphidopsis and Aphanizmenon species and one for cyrJ with around 90% identity with Raphidiopsis and Oscillatoria species. Four water bodies presented the same variant for mcyB similar to Microcystis panniformis with 99% of identity. Conclusions This study contributes new data on the presence of toxic cyanobacteria strains and provides new molecular tools to assess cyanotoxin hazards in Ecuadorian water bodies.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2022000100720
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s2179-975x2122
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Limnologia
publisher.none.fl_str_mv Associação Brasileira de Limnologia
dc.source.none.fl_str_mv Acta Limnologica Brasiliensia v.34 2022
reponame:Acta Limnologica Brasiliensia (Online)
instname:Associação Brasileira de Limnologia (ABL)
instacron:ABL
instname_str Associação Brasileira de Limnologia (ABL)
instacron_str ABL
institution ABL
reponame_str Acta Limnologica Brasiliensia (Online)
collection Acta Limnologica Brasiliensia (Online)
repository.name.fl_str_mv Acta Limnologica Brasiliensia (Online) - Associação Brasileira de Limnologia (ABL)
repository.mail.fl_str_mv ||actalb@rc.unesp.br
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