Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering

Detalhes bibliográficos
Autor(a) principal: Yang,Chunrong
Data de Publicação: 2015
Outros Autores: Fang,Cong
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000501077
Resumo: AbstractScaffolds featuring spatiotemporal control of drug release is highly desirable for bone tissue regeneration. The objective of this study was to construct a scaffold with gradient porosity and drug distribution and evaluate the effect of scaffold structure on drug release kinetics and cell bioactivity. Nano-hydroxyapatite/collagen/phosphatidylserine scaffolds embedded with steroidal saponin loaded collagen microparticles were prepared using a porogen leaching protocol. Morphological characterization showed that the scaffolds consisted of dense layer and loose layer, and pores were interconnective. The microparticles were entrapped at the center of the scaffolds follow a gradient distribution. Release kinetics correlated with the structure. The loose layer showed greater drug release amount as compared to the dense layer. Such differences in release kinetics have distinct effects on cell bioactivity. Cell proliferated much more in loose layer than that in the dense layer. Such spatial and temporal control over drug deposition and delivery within the scaffolds could provide opportunities for tissue regeneration associated with optimum drug doses at wound site, and lessen undesirable drug release and side-effects at uninjured site.
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spelling Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineeringscaffoldmicroparticlecontrolled releaseboneAbstractScaffolds featuring spatiotemporal control of drug release is highly desirable for bone tissue regeneration. The objective of this study was to construct a scaffold with gradient porosity and drug distribution and evaluate the effect of scaffold structure on drug release kinetics and cell bioactivity. Nano-hydroxyapatite/collagen/phosphatidylserine scaffolds embedded with steroidal saponin loaded collagen microparticles were prepared using a porogen leaching protocol. Morphological characterization showed that the scaffolds consisted of dense layer and loose layer, and pores were interconnective. The microparticles were entrapped at the center of the scaffolds follow a gradient distribution. Release kinetics correlated with the structure. The loose layer showed greater drug release amount as compared to the dense layer. Such differences in release kinetics have distinct effects on cell bioactivity. Cell proliferated much more in loose layer than that in the dense layer. Such spatial and temporal control over drug deposition and delivery within the scaffolds could provide opportunities for tissue regeneration associated with optimum drug doses at wound site, and lessen undesirable drug release and side-effects at uninjured site.ABM, ABC, ABPol2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000501077Materials Research v.18 n.5 2015reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/1516-1439.042115info:eu-repo/semantics/openAccessYang,ChunrongFang,Congeng2015-10-19T00:00:00Zoai:scielo:S1516-14392015000501077Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2015-10-19T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
title Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
spellingShingle Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
Yang,Chunrong
scaffold
microparticle
controlled release
bone
title_short Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
title_full Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
title_fullStr Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
title_full_unstemmed Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
title_sort Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering
author Yang,Chunrong
author_facet Yang,Chunrong
Fang,Cong
author_role author
author2 Fang,Cong
author2_role author
dc.contributor.author.fl_str_mv Yang,Chunrong
Fang,Cong
dc.subject.por.fl_str_mv scaffold
microparticle
controlled release
bone
topic scaffold
microparticle
controlled release
bone
description AbstractScaffolds featuring spatiotemporal control of drug release is highly desirable for bone tissue regeneration. The objective of this study was to construct a scaffold with gradient porosity and drug distribution and evaluate the effect of scaffold structure on drug release kinetics and cell bioactivity. Nano-hydroxyapatite/collagen/phosphatidylserine scaffolds embedded with steroidal saponin loaded collagen microparticles were prepared using a porogen leaching protocol. Morphological characterization showed that the scaffolds consisted of dense layer and loose layer, and pores were interconnective. The microparticles were entrapped at the center of the scaffolds follow a gradient distribution. Release kinetics correlated with the structure. The loose layer showed greater drug release amount as compared to the dense layer. Such differences in release kinetics have distinct effects on cell bioactivity. Cell proliferated much more in loose layer than that in the dense layer. Such spatial and temporal control over drug deposition and delivery within the scaffolds could provide opportunities for tissue regeneration associated with optimum drug doses at wound site, and lessen undesirable drug release and side-effects at uninjured site.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000501077
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000501077
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1516-1439.042115
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.18 n.5 2015
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str ABM ABC ABPOL
institution ABM ABC ABPOL
reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
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