Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose

Detalhes bibliográficos
Autor(a) principal: Brisola,Juliano
Data de Publicação: 2022
Outros Autores: Andrade,Gabriel Jonathan Sousa, Oliveira,Sabrina Alves de, Viana,Renato Marcio Ribeiro, Tischer,Paula Cristina de Sousa Faria, Tischer,Cesar Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270
Resumo: Abstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane.
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spelling Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and NanocelluloseBiodegradable polymersIndustrial applicationsNanomaterialsAbstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane.ABM, ABC, ABPol2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270Materials Research v.25 2022reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/1980-5373-mr-2021-0350info:eu-repo/semantics/openAccessBrisola,JulianoAndrade,Gabriel Jonathan SousaOliveira,Sabrina Alves deViana,Renato Marcio RibeiroTischer,Paula Cristina de Sousa FariaTischer,Cesar Augustoeng2022-02-18T00:00:00Zoai:scielo:S1516-14392022000100270Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2022-02-18T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
title Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
spellingShingle Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
Brisola,Juliano
Biodegradable polymers
Industrial applications
Nanomaterials
title_short Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
title_full Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
title_fullStr Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
title_full_unstemmed Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
title_sort Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
author Brisola,Juliano
author_facet Brisola,Juliano
Andrade,Gabriel Jonathan Sousa
Oliveira,Sabrina Alves de
Viana,Renato Marcio Ribeiro
Tischer,Paula Cristina de Sousa Faria
Tischer,Cesar Augusto
author_role author
author2 Andrade,Gabriel Jonathan Sousa
Oliveira,Sabrina Alves de
Viana,Renato Marcio Ribeiro
Tischer,Paula Cristina de Sousa Faria
Tischer,Cesar Augusto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brisola,Juliano
Andrade,Gabriel Jonathan Sousa
Oliveira,Sabrina Alves de
Viana,Renato Marcio Ribeiro
Tischer,Paula Cristina de Sousa Faria
Tischer,Cesar Augusto
dc.subject.por.fl_str_mv Biodegradable polymers
Industrial applications
Nanomaterials
topic Biodegradable polymers
Industrial applications
Nanomaterials
description Abstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/1980-5373-mr-2021-0350
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.25 2022
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
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reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
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