Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Materials research (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270 |
Resumo: | Abstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane. |
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Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and NanocelluloseBiodegradable polymersIndustrial applicationsNanomaterialsAbstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane.ABM, ABC, ABPol2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270Materials Research v.25 2022reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/1980-5373-mr-2021-0350info:eu-repo/semantics/openAccessBrisola,JulianoAndrade,Gabriel Jonathan SousaOliveira,Sabrina Alves deViana,Renato Marcio RibeiroTischer,Paula Cristina de Sousa FariaTischer,Cesar Augustoeng2022-02-18T00:00:00Zoai:scielo:S1516-14392022000100270Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2022-02-18T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.none.fl_str_mv |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
title |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
spellingShingle |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose Brisola,Juliano Biodegradable polymers Industrial applications Nanomaterials |
title_short |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
title_full |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
title_fullStr |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
title_full_unstemmed |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
title_sort |
Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose |
author |
Brisola,Juliano |
author_facet |
Brisola,Juliano Andrade,Gabriel Jonathan Sousa Oliveira,Sabrina Alves de Viana,Renato Marcio Ribeiro Tischer,Paula Cristina de Sousa Faria Tischer,Cesar Augusto |
author_role |
author |
author2 |
Andrade,Gabriel Jonathan Sousa Oliveira,Sabrina Alves de Viana,Renato Marcio Ribeiro Tischer,Paula Cristina de Sousa Faria Tischer,Cesar Augusto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Brisola,Juliano Andrade,Gabriel Jonathan Sousa Oliveira,Sabrina Alves de Viana,Renato Marcio Ribeiro Tischer,Paula Cristina de Sousa Faria Tischer,Cesar Augusto |
dc.subject.por.fl_str_mv |
Biodegradable polymers Industrial applications Nanomaterials |
topic |
Biodegradable polymers Industrial applications Nanomaterials |
description |
Abstract The cellulose from Komagataeibacter hansenii is synthesized as a thin film at the surface of glucose based media. Strong acid hydrolysis release sections of crystalline cellulose chains in nanometric scale that leads to persistent suspensions in water. The cellulose anhydro-glucose hydroxyls are suitable to receive functional groups as enzymes, and lipases have great economic value being a valuable model for protein immobilization. In this work both, the membrane of bacterial cellulose as well the nanocellulose produced trough acid hydrolysis, was functionalized with a lipase. The bacterial cellulose membranes were produced by Hestrin-Schramm medium, and nanocelluloses produced from the pristine material was characterized using techniques as 13C solid state NMR and transmission electron microscopy (TEM). The pristine membranes and nanocellulose were functionalized with succinic acid as linker, then lipase was conjugated using EDC (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride), and NHS (N-Hydroxysuccinimide). The effectiveness of the chemical process was characterized, and the lipase activity were measured. The presence of the succinic acid and amide linkage, as well physical-chemical changes on the functionalized polysaccharide. Hence, we inferred that after immobilization the enzyme maintained its activity in both cellulose and the cellulose membrane. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392022000100270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1980-5373-mr-2021-0350 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABM, ABC, ABPol |
publisher.none.fl_str_mv |
ABM, ABC, ABPol |
dc.source.none.fl_str_mv |
Materials Research v.25 2022 reponame:Materials research (São Carlos. Online) instname:Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
ABM ABC ABPOL |
institution |
ABM ABC ABPOL |
reponame_str |
Materials research (São Carlos. Online) |
collection |
Materials research (São Carlos. Online) |
repository.name.fl_str_mv |
Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
dedz@power.ufscar.br |
_version_ |
1754212680689778688 |