Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Detalhes bibliográficos
Autor(a) principal: Almeida,Arthur Corrêa de
Data de Publicação: 2015
Outros Autores: Silva,Alexandra Rodrigues Pereira da, Nakamura Filho,Arnaldo, Carvalho,Marcela Davi de, Cardoso,Antônio Valadão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000200395
Resumo: Aragonite is a metastable polymorph of calcium carbonate found in mollusk’s shells, appearing in tiles and prismatic columns, cemented in a protein matrix - mainly proteins - that acts as a framework on which the aragonite is nucleated forming nacre, besides selecting the morphology of the nucleated cristaline phase. The presence of the mineralyzing organic matrix may affect osteoinductive properties of biogenic aragonite, hypothesis tested by combinated tests, comparing viability and bioactivity of biomineralizated aragonite and nacre. Bioactivity was observed by deposition of Ca-P (presumably calcium phosphate) on the surface of samples immersed in Simulated Body Fluid; biocompatibility was verified by adhesion with VERO cells; cytotoxicity and alkaline phosphatase activity assays were performed with human adipose stem cells (hASC). Samples were characterized by scanning electron microscopy and X-ray diffraction. Both materials showed similar behaviour on bioactivity assay; in contrast, exhibited different behaviours in the presence of hASC.
id ABMABCABPOL-1_6d21caf88cc8ce840f900c8b0a7c6d5c
oai_identifier_str oai:scielo:S1516-14392015000200395
network_acronym_str ABMABCABPOL-1
network_name_str Materials research (São Carlos. Online)
repository_id_str
spelling Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and BiocompatibilityaragonitenacrebiocompatibilitybioactivityosteogenesisAragonite is a metastable polymorph of calcium carbonate found in mollusk’s shells, appearing in tiles and prismatic columns, cemented in a protein matrix - mainly proteins - that acts as a framework on which the aragonite is nucleated forming nacre, besides selecting the morphology of the nucleated cristaline phase. The presence of the mineralyzing organic matrix may affect osteoinductive properties of biogenic aragonite, hypothesis tested by combinated tests, comparing viability and bioactivity of biomineralizated aragonite and nacre. Bioactivity was observed by deposition of Ca-P (presumably calcium phosphate) on the surface of samples immersed in Simulated Body Fluid; biocompatibility was verified by adhesion with VERO cells; cytotoxicity and alkaline phosphatase activity assays were performed with human adipose stem cells (hASC). Samples were characterized by scanning electron microscopy and X-ray diffraction. Both materials showed similar behaviour on bioactivity assay; in contrast, exhibited different behaviours in the presence of hASC.ABM, ABC, ABPol2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000200395Materials Research v.18 n.2 2015reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/1516-1439.339614info:eu-repo/semantics/openAccessAlmeida,Arthur Corrêa deSilva,Alexandra Rodrigues Pereira daNakamura Filho,ArnaldoCarvalho,Marcela Davi deCardoso,Antônio Valadãoeng2016-06-10T00:00:00Zoai:scielo:S1516-14392015000200395Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2016-06-10T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
title Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
spellingShingle Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
Almeida,Arthur Corrêa de
aragonite
nacre
biocompatibility
bioactivity
osteogenesis
title_short Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
title_full Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
title_fullStr Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
title_full_unstemmed Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
title_sort Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility
author Almeida,Arthur Corrêa de
author_facet Almeida,Arthur Corrêa de
Silva,Alexandra Rodrigues Pereira da
Nakamura Filho,Arnaldo
Carvalho,Marcela Davi de
Cardoso,Antônio Valadão
author_role author
author2 Silva,Alexandra Rodrigues Pereira da
Nakamura Filho,Arnaldo
Carvalho,Marcela Davi de
Cardoso,Antônio Valadão
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Almeida,Arthur Corrêa de
Silva,Alexandra Rodrigues Pereira da
Nakamura Filho,Arnaldo
Carvalho,Marcela Davi de
Cardoso,Antônio Valadão
dc.subject.por.fl_str_mv aragonite
nacre
biocompatibility
bioactivity
osteogenesis
topic aragonite
nacre
biocompatibility
bioactivity
osteogenesis
description Aragonite is a metastable polymorph of calcium carbonate found in mollusk’s shells, appearing in tiles and prismatic columns, cemented in a protein matrix - mainly proteins - that acts as a framework on which the aragonite is nucleated forming nacre, besides selecting the morphology of the nucleated cristaline phase. The presence of the mineralyzing organic matrix may affect osteoinductive properties of biogenic aragonite, hypothesis tested by combinated tests, comparing viability and bioactivity of biomineralizated aragonite and nacre. Bioactivity was observed by deposition of Ca-P (presumably calcium phosphate) on the surface of samples immersed in Simulated Body Fluid; biocompatibility was verified by adhesion with VERO cells; cytotoxicity and alkaline phosphatase activity assays were performed with human adipose stem cells (hASC). Samples were characterized by scanning electron microscopy and X-ray diffraction. Both materials showed similar behaviour on bioactivity assay; in contrast, exhibited different behaviours in the presence of hASC.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000200395
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392015000200395
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1516-1439.339614
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.18 n.2 2015
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str ABM ABC ABPOL
institution ABM ABC ABPOL
reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
_version_ 1754212665449775104

Registros relacionados