Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes

Detalhes bibliográficos
Autor(a) principal: Weska,Raquel Farias
Data de Publicação: 2009
Outros Autores: Vieira Jr.,Wellington Carlos, Nogueira,Grínia Michelle, Beppu,Marisa Masumi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392009000200020
Resumo: Silk fibroin is a fibrous protein that has been extensively studied for application in the biomedical field, and has been used as a scaffold for bone tissue engineering. Biomaterials made of proteins are prone to physical and chemical degradation during storage; lyophilization, a drying method that consists of freezing and drying steps, is known to promote minimal changes in structure and biological activity of biomaterials. This study evaluates the effect of freezing methods on the properties of lyophilized porous silk fibroin membranes. The membranes were obtained from silk fibroin solution, frozen in liquid nitrogen or ultrafreezer, lyophilized, and then characterized by XRD, FTIR, TGA, DSC and SEM. Although the membranes presented similar physical, chemical and microstructural characteristics, quench freezing with liquid nitrogen, followed by lyophilization, promoted collapse of the membranes, while slow cooling performed by ultrafreezer preserved membrane integrity.
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spelling Effect of freezing methods on the properties of lyophilized porous silk fibroin membranessilk fibroinporous membranefreezinglyophilizationSilk fibroin is a fibrous protein that has been extensively studied for application in the biomedical field, and has been used as a scaffold for bone tissue engineering. Biomaterials made of proteins are prone to physical and chemical degradation during storage; lyophilization, a drying method that consists of freezing and drying steps, is known to promote minimal changes in structure and biological activity of biomaterials. This study evaluates the effect of freezing methods on the properties of lyophilized porous silk fibroin membranes. The membranes were obtained from silk fibroin solution, frozen in liquid nitrogen or ultrafreezer, lyophilized, and then characterized by XRD, FTIR, TGA, DSC and SEM. Although the membranes presented similar physical, chemical and microstructural characteristics, quench freezing with liquid nitrogen, followed by lyophilization, promoted collapse of the membranes, while slow cooling performed by ultrafreezer preserved membrane integrity.ABM, ABC, ABPol2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392009000200020Materials Research v.12 n.2 2009reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392009000200020info:eu-repo/semantics/openAccessWeska,Raquel FariasVieira Jr.,Wellington CarlosNogueira,Grínia MichelleBeppu,Marisa Masumieng2009-07-24T00:00:00Zoai:scielo:S1516-14392009000200020Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2009-07-24T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
title Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
spellingShingle Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
Weska,Raquel Farias
silk fibroin
porous membrane
freezing
lyophilization
title_short Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
title_full Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
title_fullStr Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
title_full_unstemmed Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
title_sort Effect of freezing methods on the properties of lyophilized porous silk fibroin membranes
author Weska,Raquel Farias
author_facet Weska,Raquel Farias
Vieira Jr.,Wellington Carlos
Nogueira,Grínia Michelle
Beppu,Marisa Masumi
author_role author
author2 Vieira Jr.,Wellington Carlos
Nogueira,Grínia Michelle
Beppu,Marisa Masumi
author2_role author
author
author
dc.contributor.author.fl_str_mv Weska,Raquel Farias
Vieira Jr.,Wellington Carlos
Nogueira,Grínia Michelle
Beppu,Marisa Masumi
dc.subject.por.fl_str_mv silk fibroin
porous membrane
freezing
lyophilization
topic silk fibroin
porous membrane
freezing
lyophilization
description Silk fibroin is a fibrous protein that has been extensively studied for application in the biomedical field, and has been used as a scaffold for bone tissue engineering. Biomaterials made of proteins are prone to physical and chemical degradation during storage; lyophilization, a drying method that consists of freezing and drying steps, is known to promote minimal changes in structure and biological activity of biomaterials. This study evaluates the effect of freezing methods on the properties of lyophilized porous silk fibroin membranes. The membranes were obtained from silk fibroin solution, frozen in liquid nitrogen or ultrafreezer, lyophilized, and then characterized by XRD, FTIR, TGA, DSC and SEM. Although the membranes presented similar physical, chemical and microstructural characteristics, quench freezing with liquid nitrogen, followed by lyophilization, promoted collapse of the membranes, while slow cooling performed by ultrafreezer preserved membrane integrity.
publishDate 2009
dc.date.none.fl_str_mv 2009-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392009000200020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392009000200020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-14392009000200020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.12 n.2 2009
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str ABM ABC ABPOL
institution ABM ABC ABPOL
reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
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