Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications

Detalhes bibliográficos
Autor(a) principal: Santos,Maria Helena
Data de Publicação: 2007
Outros Autores: Mansur,Herman Sander
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Materials research (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000400018
Resumo: In the present study two novel composites of Calcium phosphates (CaP) and Collagen (COL) were synthesized, hydroxyapatite/Collagen (HA/COL) and hydroxyapatite-btricalcium phosphate/COL (HAbTCP/COL). Collagen was extracted from bovine pericardium submitted to enzymatic digestion and purification by ion-exchange chromatography yielding high purity grade type I collagen. Biocomposites of HAP/COL and HAbTCP/COL were produced with a calcium phosphate/COL ratio of 80/20 (wt. (%)) and were characterized by chemical analysis, light microscopy and scanning electron microscopy, X ray diffraction and FT-infrared spectroscopy. SEM results of the CaP powders showed agglomerates of particles at the nanometric size range with predominantly columnar shape and average chemical composition of [Ca/P] = 1.67. FTIR analysis of collagen has confirmed the major vibrational bands associated with chemical groups like amides and hydroxyls usually found in proteins. SEM micrographs have indicated that both morphological and structural features and chemical composition of the composites were very similar to their precursors, collagen and calcium phosphate components. SDS-PAGE characterization results of protein extracted and purified has showed that bovine type I collagen was successfully obtained. Finally, the biocomposites presented a homogeneous aspect with the calcium phosphate particles aggregated to the collagen fibers. Hence, the novel developed biocomposites have high potential to be used for rebuilding small lesions in bone tissue engineering.
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spelling Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applicationsbiomaterialshydroxyapatitecollagenbiocompositesIn the present study two novel composites of Calcium phosphates (CaP) and Collagen (COL) were synthesized, hydroxyapatite/Collagen (HA/COL) and hydroxyapatite-btricalcium phosphate/COL (HAbTCP/COL). Collagen was extracted from bovine pericardium submitted to enzymatic digestion and purification by ion-exchange chromatography yielding high purity grade type I collagen. Biocomposites of HAP/COL and HAbTCP/COL were produced with a calcium phosphate/COL ratio of 80/20 (wt. (%)) and were characterized by chemical analysis, light microscopy and scanning electron microscopy, X ray diffraction and FT-infrared spectroscopy. SEM results of the CaP powders showed agglomerates of particles at the nanometric size range with predominantly columnar shape and average chemical composition of [Ca/P] = 1.67. FTIR analysis of collagen has confirmed the major vibrational bands associated with chemical groups like amides and hydroxyls usually found in proteins. SEM micrographs have indicated that both morphological and structural features and chemical composition of the composites were very similar to their precursors, collagen and calcium phosphate components. SDS-PAGE characterization results of protein extracted and purified has showed that bovine type I collagen was successfully obtained. Finally, the biocomposites presented a homogeneous aspect with the calcium phosphate particles aggregated to the collagen fibers. Hence, the novel developed biocomposites have high potential to be used for rebuilding small lesions in bone tissue engineering.ABM, ABC, ABPol2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000400018Materials Research v.10 n.4 2007reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392007000400018info:eu-repo/semantics/openAccessSantos,Maria HelenaMansur,Herman Sandereng2008-02-07T00:00:00Zoai:scielo:S1516-14392007000400018Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2008-02-07T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false
dc.title.none.fl_str_mv Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
title Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
spellingShingle Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
Santos,Maria Helena
biomaterials
hydroxyapatite
collagen
biocomposites
title_short Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
title_full Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
title_fullStr Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
title_full_unstemmed Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
title_sort Low-cost processing technology for the synthesis of calcium phosphates/collagen biocomposites for potential bone tissue engineering applications
author Santos,Maria Helena
author_facet Santos,Maria Helena
Mansur,Herman Sander
author_role author
author2 Mansur,Herman Sander
author2_role author
dc.contributor.author.fl_str_mv Santos,Maria Helena
Mansur,Herman Sander
dc.subject.por.fl_str_mv biomaterials
hydroxyapatite
collagen
biocomposites
topic biomaterials
hydroxyapatite
collagen
biocomposites
description In the present study two novel composites of Calcium phosphates (CaP) and Collagen (COL) were synthesized, hydroxyapatite/Collagen (HA/COL) and hydroxyapatite-btricalcium phosphate/COL (HAbTCP/COL). Collagen was extracted from bovine pericardium submitted to enzymatic digestion and purification by ion-exchange chromatography yielding high purity grade type I collagen. Biocomposites of HAP/COL and HAbTCP/COL were produced with a calcium phosphate/COL ratio of 80/20 (wt. (%)) and were characterized by chemical analysis, light microscopy and scanning electron microscopy, X ray diffraction and FT-infrared spectroscopy. SEM results of the CaP powders showed agglomerates of particles at the nanometric size range with predominantly columnar shape and average chemical composition of [Ca/P] = 1.67. FTIR analysis of collagen has confirmed the major vibrational bands associated with chemical groups like amides and hydroxyls usually found in proteins. SEM micrographs have indicated that both morphological and structural features and chemical composition of the composites were very similar to their precursors, collagen and calcium phosphate components. SDS-PAGE characterization results of protein extracted and purified has showed that bovine type I collagen was successfully obtained. Finally, the biocomposites presented a homogeneous aspect with the calcium phosphate particles aggregated to the collagen fibers. Hence, the novel developed biocomposites have high potential to be used for rebuilding small lesions in bone tissue engineering.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000400018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392007000400018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-14392007000400018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv Materials Research v.10 n.4 2007
reponame:Materials research (São Carlos. Online)
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:ABM ABC ABPOL
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str ABM ABC ABPOL
institution ABM ABC ABPOL
reponame_str Materials research (São Carlos. Online)
collection Materials research (São Carlos. Online)
repository.name.fl_str_mv Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv dedz@power.ufscar.br
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