Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos de neuro-psiquiatria (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2016001000810 |
Resumo: | ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes. |
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Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCRenteroviruscerebrospinal fluidreverse transcriptase polymerase chain reactionhemoglobinserythrocytesmeningitisABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes.Academia Brasileira de Neurologia - ABNEURO2016-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2016001000810Arquivos de Neuro-Psiquiatria v.74 n.10 2016reponame:Arquivos de neuro-psiquiatria (Online)instname:Academia Brasileira de Neurologiainstacron:ABNEURO10.1590/0004-282X20160119info:eu-repo/semantics/openAccessAlmeida,Sérgio Monteiro deRaboni,Sônia MaraNogueira,Meri BordignonVidal,Luine R. Renaudeng2016-10-14T00:00:00Zoai:scielo:S0004-282X2016001000810Revistahttp://www.scielo.br/anphttps://old.scielo.br/oai/scielo-oai.php||revista.arquivos@abneuro.org1678-42270004-282Xopendoar:2016-10-14T00:00Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologiafalse |
dc.title.none.fl_str_mv |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
title |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
spellingShingle |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR Almeida,Sérgio Monteiro de enterovirus cerebrospinal fluid reverse transcriptase polymerase chain reaction hemoglobins erythrocytes meningitis |
title_short |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
title_full |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
title_fullStr |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
title_full_unstemmed |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
title_sort |
Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR |
author |
Almeida,Sérgio Monteiro de |
author_facet |
Almeida,Sérgio Monteiro de Raboni,Sônia Mara Nogueira,Meri Bordignon Vidal,Luine R. Renaud |
author_role |
author |
author2 |
Raboni,Sônia Mara Nogueira,Meri Bordignon Vidal,Luine R. Renaud |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Almeida,Sérgio Monteiro de Raboni,Sônia Mara Nogueira,Meri Bordignon Vidal,Luine R. Renaud |
dc.subject.por.fl_str_mv |
enterovirus cerebrospinal fluid reverse transcriptase polymerase chain reaction hemoglobins erythrocytes meningitis |
topic |
enterovirus cerebrospinal fluid reverse transcriptase polymerase chain reaction hemoglobins erythrocytes meningitis |
description |
ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2016001000810 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2016001000810 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0004-282X20160119 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
dc.source.none.fl_str_mv |
Arquivos de Neuro-Psiquiatria v.74 n.10 2016 reponame:Arquivos de neuro-psiquiatria (Online) instname:Academia Brasileira de Neurologia instacron:ABNEURO |
instname_str |
Academia Brasileira de Neurologia |
instacron_str |
ABNEURO |
institution |
ABNEURO |
reponame_str |
Arquivos de neuro-psiquiatria (Online) |
collection |
Arquivos de neuro-psiquiatria (Online) |
repository.name.fl_str_mv |
Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologia |
repository.mail.fl_str_mv |
||revista.arquivos@abneuro.org |
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1754212781138116608 |