Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Polímeros (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282016000200144 |
Resumo: | Abstract This study evaluated extracellular production of biopolymer using fungus Mucor racemosus Fresenius and glycerol as a carbon source. Initially employing conical flasks of 500 mL containing 100 mL of cultive medium with 0.18 ± 0.03 g.L–1 of microorganisms, the results showed that the best conditions of the variables studied were: initial concentration of glycerol 50 g.L–1, fermentation time of 96 h, inoculum cultivation time of 120 h, and aeration in two stages–the first 24 hours without aeration and 72 hours fermentation with aeration of 2 vvm and 2 g.L–1 of yeast extract. The experiments conducted in a Biostat B fermenter with a 2.0 L capacity that contained 1.0 L of medium showed production of 16.35 g.L–1 gum formed and 75% glycerol consumption. These conditions produced a biopolymer with the molecular weight and total sugar content of 4.607×106 g.mol–1 (Da) and 89.5%, respectively. |
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Polímeros (São Carlos. Online) |
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Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substratebiopolymerMucor racemosus FreseniusglycerolaerationfermentationproductivityAbstract This study evaluated extracellular production of biopolymer using fungus Mucor racemosus Fresenius and glycerol as a carbon source. Initially employing conical flasks of 500 mL containing 100 mL of cultive medium with 0.18 ± 0.03 g.L–1 of microorganisms, the results showed that the best conditions of the variables studied were: initial concentration of glycerol 50 g.L–1, fermentation time of 96 h, inoculum cultivation time of 120 h, and aeration in two stages–the first 24 hours without aeration and 72 hours fermentation with aeration of 2 vvm and 2 g.L–1 of yeast extract. The experiments conducted in a Biostat B fermenter with a 2.0 L capacity that contained 1.0 L of medium showed production of 16.35 g.L–1 gum formed and 75% glycerol consumption. These conditions produced a biopolymer with the molecular weight and total sugar content of 4.607×106 g.mol–1 (Da) and 89.5%, respectively.Associação Brasileira de Polímeros2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282016000200144Polímeros v.26 n.2 2016reponame:Polímeros (São Carlos. Online)instname:Associação Brasileira de Polímeros (ABPol)instacron:ABPO10.1590/0104-1428.2294info:eu-repo/semantics/openAccessAraújo,Thaíssa RodriguesPetkowicz,Carmen Lúcia de OliveiraCardoso,Vicelma LuizCoutinho Filho,UbirajaraVieira,Patrícia Angélicaeng2016-12-20T00:00:00Zoai:scielo:S0104-14282016000200144Revistahttp://www.scielo.br/pohttps://old.scielo.br/oai/scielo-oai.php||revista@abpol.org.br1678-51690104-1428opendoar:2016-12-20T00:00Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)false |
dc.title.none.fl_str_mv |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
title |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
spellingShingle |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate Araújo,Thaíssa Rodrigues biopolymer Mucor racemosus Fresenius glycerol aeration fermentation productivity |
title_short |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
title_full |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
title_fullStr |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
title_full_unstemmed |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
title_sort |
Biopolymer production using fungus Mucor racemosus Fresenius and glycerol as substrate |
author |
Araújo,Thaíssa Rodrigues |
author_facet |
Araújo,Thaíssa Rodrigues Petkowicz,Carmen Lúcia de Oliveira Cardoso,Vicelma Luiz Coutinho Filho,Ubirajara Vieira,Patrícia Angélica |
author_role |
author |
author2 |
Petkowicz,Carmen Lúcia de Oliveira Cardoso,Vicelma Luiz Coutinho Filho,Ubirajara Vieira,Patrícia Angélica |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Araújo,Thaíssa Rodrigues Petkowicz,Carmen Lúcia de Oliveira Cardoso,Vicelma Luiz Coutinho Filho,Ubirajara Vieira,Patrícia Angélica |
dc.subject.por.fl_str_mv |
biopolymer Mucor racemosus Fresenius glycerol aeration fermentation productivity |
topic |
biopolymer Mucor racemosus Fresenius glycerol aeration fermentation productivity |
description |
Abstract This study evaluated extracellular production of biopolymer using fungus Mucor racemosus Fresenius and glycerol as a carbon source. Initially employing conical flasks of 500 mL containing 100 mL of cultive medium with 0.18 ± 0.03 g.L–1 of microorganisms, the results showed that the best conditions of the variables studied were: initial concentration of glycerol 50 g.L–1, fermentation time of 96 h, inoculum cultivation time of 120 h, and aeration in two stages–the first 24 hours without aeration and 72 hours fermentation with aeration of 2 vvm and 2 g.L–1 of yeast extract. The experiments conducted in a Biostat B fermenter with a 2.0 L capacity that contained 1.0 L of medium showed production of 16.35 g.L–1 gum formed and 75% glycerol consumption. These conditions produced a biopolymer with the molecular weight and total sugar content of 4.607×106 g.mol–1 (Da) and 89.5%, respectively. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282016000200144 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282016000200144 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-1428.2294 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
dc.source.none.fl_str_mv |
Polímeros v.26 n.2 2016 reponame:Polímeros (São Carlos. Online) instname:Associação Brasileira de Polímeros (ABPol) instacron:ABPO |
instname_str |
Associação Brasileira de Polímeros (ABPol) |
instacron_str |
ABPO |
institution |
ABPO |
reponame_str |
Polímeros (São Carlos. Online) |
collection |
Polímeros (São Carlos. Online) |
repository.name.fl_str_mv |
Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol) |
repository.mail.fl_str_mv |
||revista@abpol.org.br |
_version_ |
1754212589607321600 |