Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Polímeros (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282019000300415 |
Resumo: | Abstract Polymer nanoparticles have been widely used in many biomedical applications, constituting a major incentive for immobilization of proteins. Poly(methyl methacrylate) nanoparticles were synthesized through miniemulsion polymerizations and used as supports for bovine serum albumin immobilization. Particularly, the effects of surfactant type (anionic sodium dodecyl sulfate and cationic cetyl trimethyl ammonium bromide) surfactant concentration and monomer holdup on some of the final nanoparticle properties (particle sizes, zeta potential and protein load) were characterized with help of statistical experimental designs for the first time. Results showed that the characteristics of the surfactant controlled the BSA adsorption efficiency, with enhanced rates of adsorption on the anionic particle surfaces, showing that the surfactant exerts fundamental effect on functionalization of emulsified polymer particles, which must be explicitly acknowledged in studies of polymer particle functionalization with proteins. Finally, BSA adsorption was shown to follow a multilayer process, given the better fitting with the Freundlich model. |
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Polímeros (São Carlos. Online) |
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Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticlesmethyl methacrylateminiemulsion polymerizationsurfactantprotein adsorptionstatistical experimental designAbstract Polymer nanoparticles have been widely used in many biomedical applications, constituting a major incentive for immobilization of proteins. Poly(methyl methacrylate) nanoparticles were synthesized through miniemulsion polymerizations and used as supports for bovine serum albumin immobilization. Particularly, the effects of surfactant type (anionic sodium dodecyl sulfate and cationic cetyl trimethyl ammonium bromide) surfactant concentration and monomer holdup on some of the final nanoparticle properties (particle sizes, zeta potential and protein load) were characterized with help of statistical experimental designs for the first time. Results showed that the characteristics of the surfactant controlled the BSA adsorption efficiency, with enhanced rates of adsorption on the anionic particle surfaces, showing that the surfactant exerts fundamental effect on functionalization of emulsified polymer particles, which must be explicitly acknowledged in studies of polymer particle functionalization with proteins. Finally, BSA adsorption was shown to follow a multilayer process, given the better fitting with the Freundlich model.Associação Brasileira de Polímeros2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282019000300415Polímeros v.29 n.3 2019reponame:Polímeros (São Carlos. Online)instname:Associação Brasileira de Polímeros (ABPol)instacron:ABPO10.1590/0104-1428.05818info:eu-repo/semantics/openAccessCampos,IzabellaPaiva,ThamirisFerraz,HelenPinto,José Carloseng2019-10-30T00:00:00Zoai:scielo:S0104-14282019000300415Revistahttp://www.scielo.br/pohttps://old.scielo.br/oai/scielo-oai.php||revista@abpol.org.br1678-51690104-1428opendoar:2019-10-30T00:00Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)false |
dc.title.none.fl_str_mv |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
title |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
spellingShingle |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles Campos,Izabella methyl methacrylate miniemulsion polymerization surfactant protein adsorption statistical experimental design |
title_short |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
title_full |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
title_fullStr |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
title_full_unstemmed |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
title_sort |
Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles |
author |
Campos,Izabella |
author_facet |
Campos,Izabella Paiva,Thamiris Ferraz,Helen Pinto,José Carlos |
author_role |
author |
author2 |
Paiva,Thamiris Ferraz,Helen Pinto,José Carlos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Campos,Izabella Paiva,Thamiris Ferraz,Helen Pinto,José Carlos |
dc.subject.por.fl_str_mv |
methyl methacrylate miniemulsion polymerization surfactant protein adsorption statistical experimental design |
topic |
methyl methacrylate miniemulsion polymerization surfactant protein adsorption statistical experimental design |
description |
Abstract Polymer nanoparticles have been widely used in many biomedical applications, constituting a major incentive for immobilization of proteins. Poly(methyl methacrylate) nanoparticles were synthesized through miniemulsion polymerizations and used as supports for bovine serum albumin immobilization. Particularly, the effects of surfactant type (anionic sodium dodecyl sulfate and cationic cetyl trimethyl ammonium bromide) surfactant concentration and monomer holdup on some of the final nanoparticle properties (particle sizes, zeta potential and protein load) were characterized with help of statistical experimental designs for the first time. Results showed that the characteristics of the surfactant controlled the BSA adsorption efficiency, with enhanced rates of adsorption on the anionic particle surfaces, showing that the surfactant exerts fundamental effect on functionalization of emulsified polymer particles, which must be explicitly acknowledged in studies of polymer particle functionalization with proteins. Finally, BSA adsorption was shown to follow a multilayer process, given the better fitting with the Freundlich model. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282019000300415 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282019000300415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-1428.05818 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
dc.source.none.fl_str_mv |
Polímeros v.29 n.3 2019 reponame:Polímeros (São Carlos. Online) instname:Associação Brasileira de Polímeros (ABPol) instacron:ABPO |
instname_str |
Associação Brasileira de Polímeros (ABPol) |
instacron_str |
ABPO |
institution |
ABPO |
reponame_str |
Polímeros (São Carlos. Online) |
collection |
Polímeros (São Carlos. Online) |
repository.name.fl_str_mv |
Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol) |
repository.mail.fl_str_mv |
||revista@abpol.org.br |
_version_ |
1754212590922235904 |