Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.

Detalhes bibliográficos
Autor(a) principal: Masetto,Tathiana Elisa
Data de Publicação: 2016
Outros Autores: Fraiz,Ana Carla Resende, Faria,José Márcio Rocha
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Journal of Seed Science
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372016000300254
Resumo: Abstract During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.
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spelling Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.cell viabilitytransmission electron microscopyTUNEL testAbstract During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.ABRATES - Associação Brasileira de Tecnologia de Sementes2016-09-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372016000300254Journal of Seed Science v.38 n.3 2016reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v38n3161129info:eu-repo/semantics/openAccessMasetto,Tathiana ElisaFraiz,Ana Carla ResendeFaria,José Márcio Rochaeng2016-10-04T00:00:00Zoai:scielo:S2317-15372016000300254Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2016-10-04T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
title Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
spellingShingle Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
Masetto,Tathiana Elisa
cell viability
transmission electron microscopy
TUNEL test
title_short Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
title_full Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
title_fullStr Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
title_full_unstemmed Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
title_sort Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.
author Masetto,Tathiana Elisa
author_facet Masetto,Tathiana Elisa
Fraiz,Ana Carla Resende
Faria,José Márcio Rocha
author_role author
author2 Fraiz,Ana Carla Resende
Faria,José Márcio Rocha
author2_role author
author
dc.contributor.author.fl_str_mv Masetto,Tathiana Elisa
Fraiz,Ana Carla Resende
Faria,José Márcio Rocha
dc.subject.por.fl_str_mv cell viability
transmission electron microscopy
TUNEL test
topic cell viability
transmission electron microscopy
TUNEL test
description Abstract During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372016000300254
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372016000300254
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2317-1545v38n3161129
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Journal of Seed Science v.38 n.3 2016
reponame:Journal of Seed Science
instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)
instacron:ABRATES
instname_str Associação Brasileira de Tecnologia de Sementes (ABRATES)
instacron_str ABRATES
institution ABRATES
reponame_str Journal of Seed Science
collection Journal of Seed Science
repository.name.fl_str_mv Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)
repository.mail.fl_str_mv ||abrates@abrates.org.br
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