Cytogenetic analysis of wheat seeds submitted to artificial aging stress

Detalhes bibliográficos
Autor(a) principal: Menezes,Vanessa Ocom
Data de Publicação: 2014
Outros Autores: Lopes,Sidinei Jose, Tedesco,Solange Bosio, Henning,Fernando Augusto, Zen,Humberto Davi, Mertz,Liliane Marcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of Seed Science
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009
Resumo: Several studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges.
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spelling Cytogenetic analysis of wheat seeds submitted to artificial aging stresschromosomal abnormalitiesmitotic indexseed deteriorationTriticum aestivumartificial aging stressSeveral studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges.ABRATES - Associação Brasileira de Tecnologia de Sementes2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009Journal of Seed Science v.36 n.1 2014reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/S2317-15372014000100009info:eu-repo/semantics/openAccessMenezes,Vanessa OcomLopes,Sidinei JoseTedesco,Solange BosioHenning,Fernando AugustoZen,Humberto DaviMertz,Liliane Marciaeng2014-04-01T00:00:00Zoai:scielo:S2317-15372014000100009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2014-04-01T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Cytogenetic analysis of wheat seeds submitted to artificial aging stress
title Cytogenetic analysis of wheat seeds submitted to artificial aging stress
spellingShingle Cytogenetic analysis of wheat seeds submitted to artificial aging stress
Menezes,Vanessa Ocom
chromosomal abnormalities
mitotic index
seed deterioration
Triticum aestivum
artificial aging stress
title_short Cytogenetic analysis of wheat seeds submitted to artificial aging stress
title_full Cytogenetic analysis of wheat seeds submitted to artificial aging stress
title_fullStr Cytogenetic analysis of wheat seeds submitted to artificial aging stress
title_full_unstemmed Cytogenetic analysis of wheat seeds submitted to artificial aging stress
title_sort Cytogenetic analysis of wheat seeds submitted to artificial aging stress
author Menezes,Vanessa Ocom
author_facet Menezes,Vanessa Ocom
Lopes,Sidinei Jose
Tedesco,Solange Bosio
Henning,Fernando Augusto
Zen,Humberto Davi
Mertz,Liliane Marcia
author_role author
author2 Lopes,Sidinei Jose
Tedesco,Solange Bosio
Henning,Fernando Augusto
Zen,Humberto Davi
Mertz,Liliane Marcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes,Vanessa Ocom
Lopes,Sidinei Jose
Tedesco,Solange Bosio
Henning,Fernando Augusto
Zen,Humberto Davi
Mertz,Liliane Marcia
dc.subject.por.fl_str_mv chromosomal abnormalities
mitotic index
seed deterioration
Triticum aestivum
artificial aging stress
topic chromosomal abnormalities
mitotic index
seed deterioration
Triticum aestivum
artificial aging stress
description Several studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S2317-15372014000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Journal of Seed Science v.36 n.1 2014
reponame:Journal of Seed Science
instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)
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repository.name.fl_str_mv Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)
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