Cytogenetic analysis of wheat seeds submitted to artificial aging stress
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009 |
Resumo: | Several studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges. |
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Cytogenetic analysis of wheat seeds submitted to artificial aging stresschromosomal abnormalitiesmitotic indexseed deteriorationTriticum aestivumartificial aging stressSeveral studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges.ABRATES - Associação Brasileira de Tecnologia de Sementes2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009Journal of Seed Science v.36 n.1 2014reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/S2317-15372014000100009info:eu-repo/semantics/openAccessMenezes,Vanessa OcomLopes,Sidinei JoseTedesco,Solange BosioHenning,Fernando AugustoZen,Humberto DaviMertz,Liliane Marciaeng2014-04-01T00:00:00Zoai:scielo:S2317-15372014000100009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2014-04-01T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
title |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
spellingShingle |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress Menezes,Vanessa Ocom chromosomal abnormalities mitotic index seed deterioration Triticum aestivum artificial aging stress |
title_short |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
title_full |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
title_fullStr |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
title_full_unstemmed |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
title_sort |
Cytogenetic analysis of wheat seeds submitted to artificial aging stress |
author |
Menezes,Vanessa Ocom |
author_facet |
Menezes,Vanessa Ocom Lopes,Sidinei Jose Tedesco,Solange Bosio Henning,Fernando Augusto Zen,Humberto Davi Mertz,Liliane Marcia |
author_role |
author |
author2 |
Lopes,Sidinei Jose Tedesco,Solange Bosio Henning,Fernando Augusto Zen,Humberto Davi Mertz,Liliane Marcia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Menezes,Vanessa Ocom Lopes,Sidinei Jose Tedesco,Solange Bosio Henning,Fernando Augusto Zen,Humberto Davi Mertz,Liliane Marcia |
dc.subject.por.fl_str_mv |
chromosomal abnormalities mitotic index seed deterioration Triticum aestivum artificial aging stress |
topic |
chromosomal abnormalities mitotic index seed deterioration Triticum aestivum artificial aging stress |
description |
Several studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S2317-15372014000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.36 n.1 2014 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
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1754212981689810944 |