Molecular detection of Colletotrichum lindemuthianum in bean seed samples

Detalhes bibliográficos
Autor(a) principal: Gadaga,Stélio Jorge Castro
Data de Publicação: 2018
Outros Autores: Siqueira,Carolina da Silva, Machado,José da Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of Seed Science
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372018000400370
Resumo: Abstract: Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.
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spelling Molecular detection of Colletotrichum lindemuthianum in bean seed samplesPhaseolus vulgarisanthracnosePCRAbstract: Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.ABRATES - Associação Brasileira de Tecnologia de Sementes2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372018000400370Journal of Seed Science v.40 n.4 2018reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v40n4192761info:eu-repo/semantics/openAccessGadaga,Stélio Jorge CastroSiqueira,Carolina da SilvaMachado,José da Cruzeng2018-11-23T00:00:00Zoai:scielo:S2317-15372018000400370Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2018-11-23T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Molecular detection of Colletotrichum lindemuthianum in bean seed samples
title Molecular detection of Colletotrichum lindemuthianum in bean seed samples
spellingShingle Molecular detection of Colletotrichum lindemuthianum in bean seed samples
Gadaga,Stélio Jorge Castro
Phaseolus vulgaris
anthracnose
PCR
title_short Molecular detection of Colletotrichum lindemuthianum in bean seed samples
title_full Molecular detection of Colletotrichum lindemuthianum in bean seed samples
title_fullStr Molecular detection of Colletotrichum lindemuthianum in bean seed samples
title_full_unstemmed Molecular detection of Colletotrichum lindemuthianum in bean seed samples
title_sort Molecular detection of Colletotrichum lindemuthianum in bean seed samples
author Gadaga,Stélio Jorge Castro
author_facet Gadaga,Stélio Jorge Castro
Siqueira,Carolina da Silva
Machado,José da Cruz
author_role author
author2 Siqueira,Carolina da Silva
Machado,José da Cruz
author2_role author
author
dc.contributor.author.fl_str_mv Gadaga,Stélio Jorge Castro
Siqueira,Carolina da Silva
Machado,José da Cruz
dc.subject.por.fl_str_mv Phaseolus vulgaris
anthracnose
PCR
topic Phaseolus vulgaris
anthracnose
PCR
description Abstract: Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2317-1545v40n4192761
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dc.publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Journal of Seed Science v.40 n.4 2018
reponame:Journal of Seed Science
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