Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds

Detalhes bibliográficos
Autor(a) principal: Barrocas,Ellen Noly
Data de Publicação: 2012
Outros Autores: Machado,José da Cruz, Almeida,Mirella Figueiró de, Botelho,Luana Silva, Pinho,Édila Vilela de Resende Von
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Sementes (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
Resumo: Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.
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spelling Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seedsfungusmolecular detectionstalk and ear rotmacrospore leaf spotMaize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.Associação Brasileira de Tecnologia de Sementes2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005Revista Brasileira de Sementes v.34 n.2 2012reponame:Revista Brasileira de Sementes (Online)instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABTS10.1590/S0101-31222012000200005info:eu-repo/semantics/openAccessBarrocas,Ellen NolyMachado,José da CruzAlmeida,Mirella Figueiró deBotelho,Luana SilvaPinho,Édila Vilela de Resende Voneng2012-06-27T00:00:00Zoai:scielo:S0101-31222012000200005Revistahttp://www.abrates.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br1806-99750101-3122opendoar:2012-06-27T00:00Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
title Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
spellingShingle Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
Barrocas,Ellen Noly
fungus
molecular detection
stalk and ear rot
macrospore leaf spot
title_short Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
title_full Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
title_fullStr Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
title_full_unstemmed Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
title_sort Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
author Barrocas,Ellen Noly
author_facet Barrocas,Ellen Noly
Machado,José da Cruz
Almeida,Mirella Figueiró de
Botelho,Luana Silva
Pinho,Édila Vilela de Resende Von
author_role author
author2 Machado,José da Cruz
Almeida,Mirella Figueiró de
Botelho,Luana Silva
Pinho,Édila Vilela de Resende Von
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Barrocas,Ellen Noly
Machado,José da Cruz
Almeida,Mirella Figueiró de
Botelho,Luana Silva
Pinho,Édila Vilela de Resende Von
dc.subject.por.fl_str_mv fungus
molecular detection
stalk and ear rot
macrospore leaf spot
topic fungus
molecular detection
stalk and ear rot
macrospore leaf spot
description Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-31222012000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Revista Brasileira de Sementes v.34 n.2 2012
reponame:Revista Brasileira de Sementes (Online)
instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)
instacron:ABTS
instname_str Associação Brasileira de Tecnologia de Sementes (ABRATES)
instacron_str ABTS
institution ABTS
reponame_str Revista Brasileira de Sementes (Online)
collection Revista Brasileira de Sementes (Online)
repository.name.fl_str_mv Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)
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