Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Sementes (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005 |
Resumo: | Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species. |
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Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seedsfungusmolecular detectionstalk and ear rotmacrospore leaf spotMaize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.Associação Brasileira de Tecnologia de Sementes2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005Revista Brasileira de Sementes v.34 n.2 2012reponame:Revista Brasileira de Sementes (Online)instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABTS10.1590/S0101-31222012000200005info:eu-repo/semantics/openAccessBarrocas,Ellen NolyMachado,José da CruzAlmeida,Mirella Figueiró deBotelho,Luana SilvaPinho,Édila Vilela de Resende Voneng2012-06-27T00:00:00Zoai:scielo:S0101-31222012000200005Revistahttp://www.abrates.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br1806-99750101-3122opendoar:2012-06-27T00:00Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
title |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
spellingShingle |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds Barrocas,Ellen Noly fungus molecular detection stalk and ear rot macrospore leaf spot |
title_short |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
title_full |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
title_fullStr |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
title_full_unstemmed |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
title_sort |
Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds |
author |
Barrocas,Ellen Noly |
author_facet |
Barrocas,Ellen Noly Machado,José da Cruz Almeida,Mirella Figueiró de Botelho,Luana Silva Pinho,Édila Vilela de Resende Von |
author_role |
author |
author2 |
Machado,José da Cruz Almeida,Mirella Figueiró de Botelho,Luana Silva Pinho,Édila Vilela de Resende Von |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Barrocas,Ellen Noly Machado,José da Cruz Almeida,Mirella Figueiró de Botelho,Luana Silva Pinho,Édila Vilela de Resende Von |
dc.subject.por.fl_str_mv |
fungus molecular detection stalk and ear rot macrospore leaf spot |
topic |
fungus molecular detection stalk and ear rot macrospore leaf spot |
description |
Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-31222012000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Revista Brasileira de Sementes v.34 n.2 2012 reponame:Revista Brasileira de Sementes (Online) instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABTS |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABTS |
institution |
ABTS |
reponame_str |
Revista Brasileira de Sementes (Online) |
collection |
Revista Brasileira de Sementes (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
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1754820949870379008 |