Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds

• Autor(a) principal: Barrocas,Ellen Noly
• Data de Publicação: 2012
• Outros Autores: Machado,José da Cruz, Almeida,Mirella Figueiró de, Botelho,Luana Silva, Pinho,Édila Vilela de Resende Von
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Sementes (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
• topic: fungus; molecular detection; stalk and ear rot; macrospore leaf spot

Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.