Genetic purity certificate in seeds of hybrid maize using molecular markers
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Sementes (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024 |
Resumo: | One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis. |
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Genetic purity certificate in seeds of hybrid maize using molecular markersZea maysmicrosatellites markersisoenzymatic markersOne of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.Associação Brasileira de Tecnologia de Sementes2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024Revista Brasileira de Sementes v.28 n.1 2006reponame:Revista Brasileira de Sementes (Online)instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABTS10.1590/S0101-31222006000100024info:eu-repo/semantics/openAccessSalgado,Kalinka Carla Padovani de CarvalhoVieira,das Graças Guimarães CarvalhoVon Pinho,Édila Vilela de ResendeGuimarães,Cláudia TeixeiraVon Pinho,Renzo GarciaSouza,Lourenço Viana deeng2006-10-25T00:00:00Zoai:scielo:S0101-31222006000100024Revistahttp://www.abrates.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br1806-99750101-3122opendoar:2006-10-25T00:00Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
title |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
spellingShingle |
Genetic purity certificate in seeds of hybrid maize using molecular markers Salgado,Kalinka Carla Padovani de Carvalho Zea mays microsatellites markers isoenzymatic markers |
title_short |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
title_full |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
title_fullStr |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
title_full_unstemmed |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
title_sort |
Genetic purity certificate in seeds of hybrid maize using molecular markers |
author |
Salgado,Kalinka Carla Padovani de Carvalho |
author_facet |
Salgado,Kalinka Carla Padovani de Carvalho Vieira,das Graças Guimarães Carvalho Von Pinho,Édila Vilela de Resende Guimarães,Cláudia Teixeira Von Pinho,Renzo Garcia Souza,Lourenço Viana de |
author_role |
author |
author2 |
Vieira,das Graças Guimarães Carvalho Von Pinho,Édila Vilela de Resende Guimarães,Cláudia Teixeira Von Pinho,Renzo Garcia Souza,Lourenço Viana de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Salgado,Kalinka Carla Padovani de Carvalho Vieira,das Graças Guimarães Carvalho Von Pinho,Édila Vilela de Resende Guimarães,Cláudia Teixeira Von Pinho,Renzo Garcia Souza,Lourenço Viana de |
dc.subject.por.fl_str_mv |
Zea mays microsatellites markers isoenzymatic markers |
topic |
Zea mays microsatellites markers isoenzymatic markers |
description |
One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-31222006000100024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Revista Brasileira de Sementes v.28 n.1 2006 reponame:Revista Brasileira de Sementes (Online) instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABTS |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABTS |
institution |
ABTS |
reponame_str |
Revista Brasileira de Sementes (Online) |
collection |
Revista Brasileira de Sementes (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
_version_ |
1754820947377913856 |