Genetic purity certificate in seeds of hybrid maize using molecular markers

Detalhes bibliográficos
Autor(a) principal: Salgado,Kalinka Carla Padovani de Carvalho
Data de Publicação: 2006
Outros Autores: Vieira,das Graças Guimarães Carvalho, Von Pinho,Édila Vilela de Resende, Guimarães,Cláudia Teixeira, Von Pinho,Renzo Garcia, Souza,Lourenço Viana de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Sementes (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024
Resumo: One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.
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spelling Genetic purity certificate in seeds of hybrid maize using molecular markersZea maysmicrosatellites markersisoenzymatic markersOne of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.Associação Brasileira de Tecnologia de Sementes2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024Revista Brasileira de Sementes v.28 n.1 2006reponame:Revista Brasileira de Sementes (Online)instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABTS10.1590/S0101-31222006000100024info:eu-repo/semantics/openAccessSalgado,Kalinka Carla Padovani de CarvalhoVieira,das Graças Guimarães CarvalhoVon Pinho,Édila Vilela de ResendeGuimarães,Cláudia TeixeiraVon Pinho,Renzo GarciaSouza,Lourenço Viana deeng2006-10-25T00:00:00Zoai:scielo:S0101-31222006000100024Revistahttp://www.abrates.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br1806-99750101-3122opendoar:2006-10-25T00:00Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Genetic purity certificate in seeds of hybrid maize using molecular markers
title Genetic purity certificate in seeds of hybrid maize using molecular markers
spellingShingle Genetic purity certificate in seeds of hybrid maize using molecular markers
Salgado,Kalinka Carla Padovani de Carvalho
Zea mays
microsatellites markers
isoenzymatic markers
title_short Genetic purity certificate in seeds of hybrid maize using molecular markers
title_full Genetic purity certificate in seeds of hybrid maize using molecular markers
title_fullStr Genetic purity certificate in seeds of hybrid maize using molecular markers
title_full_unstemmed Genetic purity certificate in seeds of hybrid maize using molecular markers
title_sort Genetic purity certificate in seeds of hybrid maize using molecular markers
author Salgado,Kalinka Carla Padovani de Carvalho
author_facet Salgado,Kalinka Carla Padovani de Carvalho
Vieira,das Graças Guimarães Carvalho
Von Pinho,Édila Vilela de Resende
Guimarães,Cláudia Teixeira
Von Pinho,Renzo Garcia
Souza,Lourenço Viana de
author_role author
author2 Vieira,das Graças Guimarães Carvalho
Von Pinho,Édila Vilela de Resende
Guimarães,Cláudia Teixeira
Von Pinho,Renzo Garcia
Souza,Lourenço Viana de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Salgado,Kalinka Carla Padovani de Carvalho
Vieira,das Graças Guimarães Carvalho
Von Pinho,Édila Vilela de Resende
Guimarães,Cláudia Teixeira
Von Pinho,Renzo Garcia
Souza,Lourenço Viana de
dc.subject.por.fl_str_mv Zea mays
microsatellites markers
isoenzymatic markers
topic Zea mays
microsatellites markers
isoenzymatic markers
description One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.
publishDate 2006
dc.date.none.fl_str_mv 2006-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222006000100024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-31222006000100024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Revista Brasileira de Sementes v.28 n.1 2006
reponame:Revista Brasileira de Sementes (Online)
instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)
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instname_str Associação Brasileira de Tecnologia de Sementes (ABRATES)
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reponame_str Revista Brasileira de Sementes (Online)
collection Revista Brasileira de Sementes (Online)
repository.name.fl_str_mv Revista Brasileira de Sementes (Online) - Associação Brasileira de Tecnologia de Sementes (ABRATES)
repository.mail.fl_str_mv ||abrates@abrates.org.br
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