Identification of hepatitis B virus genotypes in the state of São Paulo
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Associação Médica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500424 |
Resumo: | Objective: the aim of this study was to identify HBV genotypes in serum samples from patients from the state of São Paulo, received by the viral hepatitis laboratory, at the Virology Centre of Instituto Adolfo Lutz, from various municipalities. Methods: a total of 94 serum samples were randomly analyzed. Genotyping was performed using nested PCR for amplification of S and Pol regions from viral genome. Genotypes were identified comparing the sequences obtained with the sequences deposited in GenBank. Results: we were able to determine the genotype of 91 (97%) samples, as follows: genotype A (55.3%), D (32%), F (5.3%), C (3.2%) and G (1%). There are few data on the epidemiology of genotype G. This genotype has been detected in restricted areas around the world. Frequently, the genotype G infection occurs in HIV-positive male patients. In our case, the sample identified as G was also positive for HIV but in a female patient, which is an uncommon finding in the scientific literature. Conclusion: in this work, we identified the most frequent genotypes in São Paulo as well as the genotype G, rare among the genotypes found in our environment. |
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Revista da Associação Médica Brasileira (Online) |
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Identification of hepatitis B virus genotypes in the state of São Paulohepatitis Bpolymerase chain reactiongenotypeBrazil Objective: the aim of this study was to identify HBV genotypes in serum samples from patients from the state of São Paulo, received by the viral hepatitis laboratory, at the Virology Centre of Instituto Adolfo Lutz, from various municipalities. Methods: a total of 94 serum samples were randomly analyzed. Genotyping was performed using nested PCR for amplification of S and Pol regions from viral genome. Genotypes were identified comparing the sequences obtained with the sequences deposited in GenBank. Results: we were able to determine the genotype of 91 (97%) samples, as follows: genotype A (55.3%), D (32%), F (5.3%), C (3.2%) and G (1%). There are few data on the epidemiology of genotype G. This genotype has been detected in restricted areas around the world. Frequently, the genotype G infection occurs in HIV-positive male patients. In our case, the sample identified as G was also positive for HIV but in a female patient, which is an uncommon finding in the scientific literature. Conclusion: in this work, we identified the most frequent genotypes in São Paulo as well as the genotype G, rare among the genotypes found in our environment. Associação Médica Brasileira2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500424Revista da Associação Médica Brasileira v.60 n.5 2014reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.60.05.009info:eu-repo/semantics/openAccessSantos,Ana Paula de TorresLevi,José EduardoLemos,Marcílio FigueiredoCalux,Samira JulienOba,Isabel TakanoMoreira,Regina Céliaeng2014-11-17T00:00:00Zoai:scielo:S0104-42302014000500424Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2014-11-17T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false |
dc.title.none.fl_str_mv |
Identification of hepatitis B virus genotypes in the state of São Paulo |
title |
Identification of hepatitis B virus genotypes in the state of São Paulo |
spellingShingle |
Identification of hepatitis B virus genotypes in the state of São Paulo Santos,Ana Paula de Torres hepatitis B polymerase chain reaction genotype Brazil |
title_short |
Identification of hepatitis B virus genotypes in the state of São Paulo |
title_full |
Identification of hepatitis B virus genotypes in the state of São Paulo |
title_fullStr |
Identification of hepatitis B virus genotypes in the state of São Paulo |
title_full_unstemmed |
Identification of hepatitis B virus genotypes in the state of São Paulo |
title_sort |
Identification of hepatitis B virus genotypes in the state of São Paulo |
author |
Santos,Ana Paula de Torres |
author_facet |
Santos,Ana Paula de Torres Levi,José Eduardo Lemos,Marcílio Figueiredo Calux,Samira Julien Oba,Isabel Takano Moreira,Regina Célia |
author_role |
author |
author2 |
Levi,José Eduardo Lemos,Marcílio Figueiredo Calux,Samira Julien Oba,Isabel Takano Moreira,Regina Célia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Ana Paula de Torres Levi,José Eduardo Lemos,Marcílio Figueiredo Calux,Samira Julien Oba,Isabel Takano Moreira,Regina Célia |
dc.subject.por.fl_str_mv |
hepatitis B polymerase chain reaction genotype Brazil |
topic |
hepatitis B polymerase chain reaction genotype Brazil |
description |
Objective: the aim of this study was to identify HBV genotypes in serum samples from patients from the state of São Paulo, received by the viral hepatitis laboratory, at the Virology Centre of Instituto Adolfo Lutz, from various municipalities. Methods: a total of 94 serum samples were randomly analyzed. Genotyping was performed using nested PCR for amplification of S and Pol regions from viral genome. Genotypes were identified comparing the sequences obtained with the sequences deposited in GenBank. Results: we were able to determine the genotype of 91 (97%) samples, as follows: genotype A (55.3%), D (32%), F (5.3%), C (3.2%) and G (1%). There are few data on the epidemiology of genotype G. This genotype has been detected in restricted areas around the world. Frequently, the genotype G infection occurs in HIV-positive male patients. In our case, the sample identified as G was also positive for HIV but in a female patient, which is an uncommon finding in the scientific literature. Conclusion: in this work, we identified the most frequent genotypes in São Paulo as well as the genotype G, rare among the genotypes found in our environment. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500424 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500424 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1806-9282.60.05.009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Médica Brasileira |
publisher.none.fl_str_mv |
Associação Médica Brasileira |
dc.source.none.fl_str_mv |
Revista da Associação Médica Brasileira v.60 n.5 2014 reponame:Revista da Associação Médica Brasileira (Online) instname:Associação Médica Brasileira (AMB) instacron:AMB |
instname_str |
Associação Médica Brasileira (AMB) |
instacron_str |
AMB |
institution |
AMB |
reponame_str |
Revista da Associação Médica Brasileira (Online) |
collection |
Revista da Associação Médica Brasileira (Online) |
repository.name.fl_str_mv |
Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB) |
repository.mail.fl_str_mv |
||ramb@amb.org.br |
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1754212831176163328 |