Human polyomavirus infection: Cytological and molecular diagnosis

Detalhes bibliográficos
Autor(a) principal: Assis,Patricia Gioia de
Data de Publicação: 2017
Outros Autores: Carvalho,Maria da Glória da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302017001100943
Resumo: Summary Few studies directly compare urinary cytology with molecular methods for detecting BK and JC polyomaviruses. Reactivation of BKV infection is the main risk factor for the development of nephropathy in immunocompromised individuals. The limitation of the cytological method can be attributed to the stage where the infected cell does not have specific and sufficient morphological characteristics for a conclusive diagnosis and can be easily interpreted as degenerative alteration. Moreover, morphologically, it is not possible to differentiate the two types of viruses. Polymerase chain reaction (PCR), not only is a sensitive method, but also allows differentiation of viral types without quantification, and therefore is not indicative of nephropathy. According to the American Society of Nephrology, real-time PCR would be the gold standard to indicate nephropathy because it allows quantifying the number of viral copies.
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spelling Human polyomavirus infection: Cytological and molecular diagnosispolyomavirusBK virusJC viruskidney transplantationSummary Few studies directly compare urinary cytology with molecular methods for detecting BK and JC polyomaviruses. Reactivation of BKV infection is the main risk factor for the development of nephropathy in immunocompromised individuals. The limitation of the cytological method can be attributed to the stage where the infected cell does not have specific and sufficient morphological characteristics for a conclusive diagnosis and can be easily interpreted as degenerative alteration. Moreover, morphologically, it is not possible to differentiate the two types of viruses. Polymerase chain reaction (PCR), not only is a sensitive method, but also allows differentiation of viral types without quantification, and therefore is not indicative of nephropathy. According to the American Society of Nephrology, real-time PCR would be the gold standard to indicate nephropathy because it allows quantifying the number of viral copies.Associação Médica Brasileira2017-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302017001100943Revista da Associação Médica Brasileira v.63 n.11 2017reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.63.11.943info:eu-repo/semantics/openAccessAssis,Patricia Gioia deCarvalho,Maria da Glória da Costaeng2018-02-09T00:00:00Zoai:scielo:S0104-42302017001100943Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2018-02-09T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv Human polyomavirus infection: Cytological and molecular diagnosis
title Human polyomavirus infection: Cytological and molecular diagnosis
spellingShingle Human polyomavirus infection: Cytological and molecular diagnosis
Assis,Patricia Gioia de
polyomavirus
BK virus
JC virus
kidney transplantation
title_short Human polyomavirus infection: Cytological and molecular diagnosis
title_full Human polyomavirus infection: Cytological and molecular diagnosis
title_fullStr Human polyomavirus infection: Cytological and molecular diagnosis
title_full_unstemmed Human polyomavirus infection: Cytological and molecular diagnosis
title_sort Human polyomavirus infection: Cytological and molecular diagnosis
author Assis,Patricia Gioia de
author_facet Assis,Patricia Gioia de
Carvalho,Maria da Glória da Costa
author_role author
author2 Carvalho,Maria da Glória da Costa
author2_role author
dc.contributor.author.fl_str_mv Assis,Patricia Gioia de
Carvalho,Maria da Glória da Costa
dc.subject.por.fl_str_mv polyomavirus
BK virus
JC virus
kidney transplantation
topic polyomavirus
BK virus
JC virus
kidney transplantation
description Summary Few studies directly compare urinary cytology with molecular methods for detecting BK and JC polyomaviruses. Reactivation of BKV infection is the main risk factor for the development of nephropathy in immunocompromised individuals. The limitation of the cytological method can be attributed to the stage where the infected cell does not have specific and sufficient morphological characteristics for a conclusive diagnosis and can be easily interpreted as degenerative alteration. Moreover, morphologically, it is not possible to differentiate the two types of viruses. Polymerase chain reaction (PCR), not only is a sensitive method, but also allows differentiation of viral types without quantification, and therefore is not indicative of nephropathy. According to the American Society of Nephrology, real-time PCR would be the gold standard to indicate nephropathy because it allows quantifying the number of viral copies.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302017001100943
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9282.63.11.943
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dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.63 n.11 2017
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repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
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