Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Associação Médica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022 |
Resumo: | OBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin. |
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Revista da Associação Médica Brasileira (Online) |
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Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic backgroundFree fetal DNAPCRRHD genotypingMaternal plasmaPrenatal diagnosisOBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.Associação Médica Brasileira2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022Revista da Associação Médica Brasileira v.52 n.4 2006reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/S0104-42302006000400022info:eu-repo/semantics/openAccessMachado,Isabela NellyCastilho,LilianPellegrino Jr.,JordãoBarini,Ricardoeng2006-09-06T00:00:00Zoai:scielo:S0104-42302006000400022Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2006-09-06T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false |
dc.title.none.fl_str_mv |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
title |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
spellingShingle |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background Machado,Isabela Nelly Free fetal DNA PCR RHD genotyping Maternal plasma Prenatal diagnosis |
title_short |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
title_full |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
title_fullStr |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
title_full_unstemmed |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
title_sort |
Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background |
author |
Machado,Isabela Nelly |
author_facet |
Machado,Isabela Nelly Castilho,Lilian Pellegrino Jr.,Jordão Barini,Ricardo |
author_role |
author |
author2 |
Castilho,Lilian Pellegrino Jr.,Jordão Barini,Ricardo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Machado,Isabela Nelly Castilho,Lilian Pellegrino Jr.,Jordão Barini,Ricardo |
dc.subject.por.fl_str_mv |
Free fetal DNA PCR RHD genotyping Maternal plasma Prenatal diagnosis |
topic |
Free fetal DNA PCR RHD genotyping Maternal plasma Prenatal diagnosis |
description |
OBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-42302006000400022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Médica Brasileira |
publisher.none.fl_str_mv |
Associação Médica Brasileira |
dc.source.none.fl_str_mv |
Revista da Associação Médica Brasileira v.52 n.4 2006 reponame:Revista da Associação Médica Brasileira (Online) instname:Associação Médica Brasileira (AMB) instacron:AMB |
instname_str |
Associação Médica Brasileira (AMB) |
instacron_str |
AMB |
institution |
AMB |
reponame_str |
Revista da Associação Médica Brasileira (Online) |
collection |
Revista da Associação Médica Brasileira (Online) |
repository.name.fl_str_mv |
Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB) |
repository.mail.fl_str_mv |
||ramb@amb.org.br |
_version_ |
1754212827153825792 |