Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background

Detalhes bibliográficos
Autor(a) principal: Machado,Isabela Nelly
Data de Publicação: 2006
Outros Autores: Castilho,Lilian, Pellegrino Jr.,Jordão, Barini,Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022
Resumo: OBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.
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spelling Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic backgroundFree fetal DNAPCRRHD genotypingMaternal plasmaPrenatal diagnosisOBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.Associação Médica Brasileira2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022Revista da Associação Médica Brasileira v.52 n.4 2006reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/S0104-42302006000400022info:eu-repo/semantics/openAccessMachado,Isabela NellyCastilho,LilianPellegrino Jr.,JordãoBarini,Ricardoeng2006-09-06T00:00:00Zoai:scielo:S0104-42302006000400022Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2006-09-06T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
title Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
spellingShingle Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
Machado,Isabela Nelly
Free fetal DNA
PCR
RHD genotyping
Maternal plasma
Prenatal diagnosis
title_short Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
title_full Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
title_fullStr Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
title_full_unstemmed Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
title_sort Fetal rhd genotyping from maternal plasma in a population with a highly diverse ethnic background
author Machado,Isabela Nelly
author_facet Machado,Isabela Nelly
Castilho,Lilian
Pellegrino Jr.,Jordão
Barini,Ricardo
author_role author
author2 Castilho,Lilian
Pellegrino Jr.,Jordão
Barini,Ricardo
author2_role author
author
author
dc.contributor.author.fl_str_mv Machado,Isabela Nelly
Castilho,Lilian
Pellegrino Jr.,Jordão
Barini,Ricardo
dc.subject.por.fl_str_mv Free fetal DNA
PCR
RHD genotyping
Maternal plasma
Prenatal diagnosis
topic Free fetal DNA
PCR
RHD genotyping
Maternal plasma
Prenatal diagnosis
description OBJECTIVE: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. METHODS: We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. RESULTS: Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. CONCLUSIONS: Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.
publishDate 2006
dc.date.none.fl_str_mv 2006-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302006000400022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-42302006000400022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.52 n.4 2006
reponame:Revista da Associação Médica Brasileira (Online)
instname:Associação Médica Brasileira (AMB)
instacron:AMB
instname_str Associação Médica Brasileira (AMB)
instacron_str AMB
institution AMB
reponame_str Revista da Associação Médica Brasileira (Online)
collection Revista da Associação Médica Brasileira (Online)
repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
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