Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | São Paulo medical journal (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802000000200005 |
Resumo: | CONTEXT: Mutations of the p53 tumor suppressor gene are the most frequent alterations observed in human neoplasias affecting adults. In pediatric oncology, however, they have seldom been identified. Wilms tumor is a renal neoplasia commonly occurring in children and is associated with mutations of the WT1 gene. The correlation between Wilms tumor and alterations of the p53 gene has not been well established, with a low frequency of mutations having been reported in this type of tumor. Mutation may be associated with advanced stage disease and unfavorable histology. OBJECTIVE: To screen for mutations of the p53 gene by the PCR-SSCP method and DNA sequencing in cases of Wilms tumor sug-gestive of mutation. DESIGN: Case Report. CASE REPORT: Evaluations of exons 5-9 of the p53 gene in DNA samples extracted by PCR-SSCP from 10 Wilms tumors in children at different stages, and DNA sequencing. Changes in SSCP analy-sis were observed in exon 8 in two samples. The probable muta-tions were not confirmed by DNA sequencing. The absence of point mutations in p53 gene observed in the 10 samples of Wilms tumor studied agrees with literature data, with DNA sequencing being of fundamental importance for the confirmation of possible mutations. |
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Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumorWilms tumorp53 genePCR-SSCPSolid tumorsCONTEXT: Mutations of the p53 tumor suppressor gene are the most frequent alterations observed in human neoplasias affecting adults. In pediatric oncology, however, they have seldom been identified. Wilms tumor is a renal neoplasia commonly occurring in children and is associated with mutations of the WT1 gene. The correlation between Wilms tumor and alterations of the p53 gene has not been well established, with a low frequency of mutations having been reported in this type of tumor. Mutation may be associated with advanced stage disease and unfavorable histology. OBJECTIVE: To screen for mutations of the p53 gene by the PCR-SSCP method and DNA sequencing in cases of Wilms tumor sug-gestive of mutation. DESIGN: Case Report. CASE REPORT: Evaluations of exons 5-9 of the p53 gene in DNA samples extracted by PCR-SSCP from 10 Wilms tumors in children at different stages, and DNA sequencing. Changes in SSCP analy-sis were observed in exon 8 in two samples. The probable muta-tions were not confirmed by DNA sequencing. The absence of point mutations in p53 gene observed in the 10 samples of Wilms tumor studied agrees with literature data, with DNA sequencing being of fundamental importance for the confirmation of possible mutations.Associação Paulista de Medicina - APM2000-03-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802000000200005Sao Paulo Medical Journal v.118 n.2 2000reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APM10.1590/S1516-31802000000200005info:eu-repo/semantics/openAccessDefavery,RicardoLemos,José Alexandre RodriguesKashima,SimoneBernardes,José EduardoScridelli,Carlos AlbertoCovas,Dimas TadeuTone,Luiz Gonzagaeng2000-05-11T00:00:00Zoai:scielo:S1516-31802000000200005Revistahttp://www.scielo.br/spmjhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2000-05-11T00:00São Paulo medical journal (Online) - Associação Paulista de Medicinafalse |
dc.title.none.fl_str_mv |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
title |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
spellingShingle |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor Defavery,Ricardo Wilms tumor p53 gene PCR-SSCP Solid tumors |
title_short |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
title_full |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
title_fullStr |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
title_full_unstemmed |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
title_sort |
Analysis of the p53 gene by PCR-SSCP in ten cases of Wilms tumor |
author |
Defavery,Ricardo |
author_facet |
Defavery,Ricardo Lemos,José Alexandre Rodrigues Kashima,Simone Bernardes,José Eduardo Scridelli,Carlos Alberto Covas,Dimas Tadeu Tone,Luiz Gonzaga |
author_role |
author |
author2 |
Lemos,José Alexandre Rodrigues Kashima,Simone Bernardes,José Eduardo Scridelli,Carlos Alberto Covas,Dimas Tadeu Tone,Luiz Gonzaga |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Defavery,Ricardo Lemos,José Alexandre Rodrigues Kashima,Simone Bernardes,José Eduardo Scridelli,Carlos Alberto Covas,Dimas Tadeu Tone,Luiz Gonzaga |
dc.subject.por.fl_str_mv |
Wilms tumor p53 gene PCR-SSCP Solid tumors |
topic |
Wilms tumor p53 gene PCR-SSCP Solid tumors |
description |
CONTEXT: Mutations of the p53 tumor suppressor gene are the most frequent alterations observed in human neoplasias affecting adults. In pediatric oncology, however, they have seldom been identified. Wilms tumor is a renal neoplasia commonly occurring in children and is associated with mutations of the WT1 gene. The correlation between Wilms tumor and alterations of the p53 gene has not been well established, with a low frequency of mutations having been reported in this type of tumor. Mutation may be associated with advanced stage disease and unfavorable histology. OBJECTIVE: To screen for mutations of the p53 gene by the PCR-SSCP method and DNA sequencing in cases of Wilms tumor sug-gestive of mutation. DESIGN: Case Report. CASE REPORT: Evaluations of exons 5-9 of the p53 gene in DNA samples extracted by PCR-SSCP from 10 Wilms tumors in children at different stages, and DNA sequencing. Changes in SSCP analy-sis were observed in exon 8 in two samples. The probable muta-tions were not confirmed by DNA sequencing. The absence of point mutations in p53 gene observed in the 10 samples of Wilms tumor studied agrees with literature data, with DNA sequencing being of fundamental importance for the confirmation of possible mutations. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802000000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802000000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-31802000000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
dc.source.none.fl_str_mv |
Sao Paulo Medical Journal v.118 n.2 2000 reponame:São Paulo medical journal (Online) instname:Associação Paulista de Medicina instacron:APM |
instname_str |
Associação Paulista de Medicina |
instacron_str |
APM |
institution |
APM |
reponame_str |
São Paulo medical journal (Online) |
collection |
São Paulo medical journal (Online) |
repository.name.fl_str_mv |
São Paulo medical journal (Online) - Associação Paulista de Medicina |
repository.mail.fl_str_mv |
revistas@apm.org.br |
_version_ |
1754209260034588672 |