Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga

Detalhes bibliográficos
Autor(a) principal: Silva, Keila Tamires da
Data de Publicação: 2020
Outros Autores: Marinho, Ketsia Sabrina do Nascimento, Antonio, Erivaldo Alves, Júnior, Francisco Carlos Amanajás de Aguiar, Santos, Noemia Pereira da Silva, Silva, Nicácio Henrique da, Silva, Márcia Vanusa da, Santos, Katharine Raquel Pereira dos, Leite, Sônia Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Health Review
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026
Resumo: The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres.
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spelling Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de PlgaEmbryophetotoxicityEncapsulationEndochondral ossificationUsnic acid.The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres.Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/1802610.34119/bjhrv3n5-227Brazilian Journal of Health Review; Vol. 3 No. 5 (2020); 14250-14266Brazilian Journal of Health Review; v. 3 n. 5 (2020); 14250-142662595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026/14577Copyright (c) 2020 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessSilva, Keila Tamires daMarinho, Ketsia Sabrina do NascimentoAntonio, Erivaldo AlvesJúnior, Francisco Carlos Amanajás de AguiarSantos, Noemia Pereira da SilvaSilva, Nicácio Henrique daSilva, Márcia Vanusa daSantos, Katharine Raquel Pereira dosLeite, Sônia Pereira2020-11-02T15:54:24Zoai:ojs2.ojs.brazilianjournals.com.br:article/18026Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2020-11-02T15:54:24Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false
dc.title.none.fl_str_mv Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
title Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
spellingShingle Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
Silva, Keila Tamires da
Embryophetotoxicity
Encapsulation
Endochondral ossification
Usnic acid.
title_short Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
title_full Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
title_fullStr Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
title_full_unstemmed Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
title_sort Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
author Silva, Keila Tamires da
author_facet Silva, Keila Tamires da
Marinho, Ketsia Sabrina do Nascimento
Antonio, Erivaldo Alves
Júnior, Francisco Carlos Amanajás de Aguiar
Santos, Noemia Pereira da Silva
Silva, Nicácio Henrique da
Silva, Márcia Vanusa da
Santos, Katharine Raquel Pereira dos
Leite, Sônia Pereira
author_role author
author2 Marinho, Ketsia Sabrina do Nascimento
Antonio, Erivaldo Alves
Júnior, Francisco Carlos Amanajás de Aguiar
Santos, Noemia Pereira da Silva
Silva, Nicácio Henrique da
Silva, Márcia Vanusa da
Santos, Katharine Raquel Pereira dos
Leite, Sônia Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Keila Tamires da
Marinho, Ketsia Sabrina do Nascimento
Antonio, Erivaldo Alves
Júnior, Francisco Carlos Amanajás de Aguiar
Santos, Noemia Pereira da Silva
Silva, Nicácio Henrique da
Silva, Márcia Vanusa da
Santos, Katharine Raquel Pereira dos
Leite, Sônia Pereira
dc.subject.por.fl_str_mv Embryophetotoxicity
Encapsulation
Endochondral ossification
Usnic acid.
topic Embryophetotoxicity
Encapsulation
Endochondral ossification
Usnic acid.
description The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026
10.34119/bjhrv3n5-227
url https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026
identifier_str_mv 10.34119/bjhrv3n5-227
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026/14577
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Health Review
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Health Review
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Health Review; Vol. 3 No. 5 (2020); 14250-14266
Brazilian Journal of Health Review; v. 3 n. 5 (2020); 14250-14266
2595-6825
reponame:Brazilian Journal of Health Review
instname:Federação das Indústrias do Estado do Paraná (FIEP)
instacron:BJRH
instname_str Federação das Indústrias do Estado do Paraná (FIEP)
instacron_str BJRH
institution BJRH
reponame_str Brazilian Journal of Health Review
collection Brazilian Journal of Health Review
repository.name.fl_str_mv Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)
repository.mail.fl_str_mv || brazilianjhr@gmail.com
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