Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026 |
Resumo: | The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres. |
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Brazilian Journal of Health Review |
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Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de PlgaEmbryophetotoxicityEncapsulationEndochondral ossificationUsnic acid.The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres.Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/1802610.34119/bjhrv3n5-227Brazilian Journal of Health Review; Vol. 3 No. 5 (2020); 14250-14266Brazilian Journal of Health Review; v. 3 n. 5 (2020); 14250-142662595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026/14577Copyright (c) 2020 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessSilva, Keila Tamires daMarinho, Ketsia Sabrina do NascimentoAntonio, Erivaldo AlvesJúnior, Francisco Carlos Amanajás de AguiarSantos, Noemia Pereira da SilvaSilva, Nicácio Henrique daSilva, Márcia Vanusa daSantos, Katharine Raquel Pereira dosLeite, Sônia Pereira2020-11-02T15:54:24Zoai:ojs2.ojs.brazilianjournals.com.br:article/18026Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2020-11-02T15:54:24Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
title |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
spellingShingle |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga Silva, Keila Tamires da Embryophetotoxicity Encapsulation Endochondral ossification Usnic acid. |
title_short |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
title_full |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
title_fullStr |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
title_full_unstemmed |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
title_sort |
Histomorfometric Analysis of the Endochondral Ossification of the limbs of Rats submitted to Usnic Acid from Cladonia Substellata (AHTI) encapsulated in Plga-Microspheres / Análise Histomorfométrica da Ossificação Endocondral dos membros de Ratos submetidos ao Ácido Úsnico de Cladonia Substellata (AHTI) encapsulado em Microesferas de Plga |
author |
Silva, Keila Tamires da |
author_facet |
Silva, Keila Tamires da Marinho, Ketsia Sabrina do Nascimento Antonio, Erivaldo Alves Júnior, Francisco Carlos Amanajás de Aguiar Santos, Noemia Pereira da Silva Silva, Nicácio Henrique da Silva, Márcia Vanusa da Santos, Katharine Raquel Pereira dos Leite, Sônia Pereira |
author_role |
author |
author2 |
Marinho, Ketsia Sabrina do Nascimento Antonio, Erivaldo Alves Júnior, Francisco Carlos Amanajás de Aguiar Santos, Noemia Pereira da Silva Silva, Nicácio Henrique da Silva, Márcia Vanusa da Santos, Katharine Raquel Pereira dos Leite, Sônia Pereira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Keila Tamires da Marinho, Ketsia Sabrina do Nascimento Antonio, Erivaldo Alves Júnior, Francisco Carlos Amanajás de Aguiar Santos, Noemia Pereira da Silva Silva, Nicácio Henrique da Silva, Márcia Vanusa da Santos, Katharine Raquel Pereira dos Leite, Sônia Pereira |
dc.subject.por.fl_str_mv |
Embryophetotoxicity Encapsulation Endochondral ossification Usnic acid. |
topic |
Embryophetotoxicity Encapsulation Endochondral ossification Usnic acid. |
description |
The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGA-microspheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026 10.34119/bjhrv3n5-227 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026 |
identifier_str_mv |
10.34119/bjhrv3n5-227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/18026/14577 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Health Review info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Health Review |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 3 No. 5 (2020); 14250-14266 Brazilian Journal of Health Review; v. 3 n. 5 (2020); 14250-14266 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
_version_ |
1797240055894376448 |