Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
Autor(a) principal: | |
---|---|
Data de Publicação: | 2024 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875 |
Resumo: | Infections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens. |
id |
BJRH-0_143fb48b1888ac4b2de4e843ec147fe0 |
---|---|
oai_identifier_str |
oai:ojs2.ojs.brazilianjournals.com.br:article/66875 |
network_acronym_str |
BJRH-0 |
network_name_str |
Brazilian Journal of Health Review |
repository_id_str |
|
spelling |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human virusesLAMPvirusinfectionsmolecular diagnosisInfections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens.Brazilian Journals Publicações de Periódicos e Editora Ltda.2024-02-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/6687510.34119/bjhrv7n1-321Brazilian Journal of Health Review; Vol. 7 No. 1 (2024); 3958-3974Brazilian Journal of Health Review; Vol. 7 Núm. 1 (2024); 3958-3974Brazilian Journal of Health Review; v. 7 n. 1 (2024); 3958-39742595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875/47669da Silva, Yury Yzabellade Melo, Fabio LopesXavier, Edeneide MariaCoimbra, Eliane Camposinfo:eu-repo/semantics/openAccess2024-02-02T12:10:16Zoai:ojs2.ojs.brazilianjournals.com.br:article/66875Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2024-02-02T12:10:16Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
title |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
spellingShingle |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses da Silva, Yury Yzabella LAMP virus infections molecular diagnosis |
title_short |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
title_full |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
title_fullStr |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
title_full_unstemmed |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
title_sort |
Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses |
author |
da Silva, Yury Yzabella |
author_facet |
da Silva, Yury Yzabella de Melo, Fabio Lopes Xavier, Edeneide Maria Coimbra, Eliane Campos |
author_role |
author |
author2 |
de Melo, Fabio Lopes Xavier, Edeneide Maria Coimbra, Eliane Campos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
da Silva, Yury Yzabella de Melo, Fabio Lopes Xavier, Edeneide Maria Coimbra, Eliane Campos |
dc.subject.por.fl_str_mv |
LAMP virus infections molecular diagnosis |
topic |
LAMP virus infections molecular diagnosis |
description |
Infections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875 10.34119/bjhrv7n1-321 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875 |
identifier_str_mv |
10.34119/bjhrv7n1-321 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875/47669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 7 No. 1 (2024); 3958-3974 Brazilian Journal of Health Review; Vol. 7 Núm. 1 (2024); 3958-3974 Brazilian Journal of Health Review; v. 7 n. 1 (2024); 3958-3974 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
_version_ |
1797240041936781312 |