Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses

Detalhes bibliográficos
Autor(a) principal: da Silva, Yury Yzabella
Data de Publicação: 2024
Outros Autores: de Melo, Fabio Lopes, Xavier, Edeneide Maria, Coimbra, Eliane Campos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Health Review
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875
Resumo: Infections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens.
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spelling Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human virusesLAMPvirusinfectionsmolecular diagnosisInfections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens.Brazilian Journals Publicações de Periódicos e Editora Ltda.2024-02-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/6687510.34119/bjhrv7n1-321Brazilian Journal of Health Review; Vol. 7 No. 1 (2024); 3958-3974Brazilian Journal of Health Review; Vol. 7 Núm. 1 (2024); 3958-3974Brazilian Journal of Health Review; v. 7 n. 1 (2024); 3958-39742595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875/47669da Silva, Yury Yzabellade Melo, Fabio LopesXavier, Edeneide MariaCoimbra, Eliane Camposinfo:eu-repo/semantics/openAccess2024-02-02T12:10:16Zoai:ojs2.ojs.brazilianjournals.com.br:article/66875Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2024-02-02T12:10:16Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false
dc.title.none.fl_str_mv Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
title Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
spellingShingle Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
da Silva, Yury Yzabella
LAMP
virus
infections
molecular diagnosis
title_short Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
title_full Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
title_fullStr Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
title_full_unstemmed Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
title_sort Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses
author da Silva, Yury Yzabella
author_facet da Silva, Yury Yzabella
de Melo, Fabio Lopes
Xavier, Edeneide Maria
Coimbra, Eliane Campos
author_role author
author2 de Melo, Fabio Lopes
Xavier, Edeneide Maria
Coimbra, Eliane Campos
author2_role author
author
author
dc.contributor.author.fl_str_mv da Silva, Yury Yzabella
de Melo, Fabio Lopes
Xavier, Edeneide Maria
Coimbra, Eliane Campos
dc.subject.por.fl_str_mv LAMP
virus
infections
molecular diagnosis
topic LAMP
virus
infections
molecular diagnosis
description Infections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875
10.34119/bjhrv7n1-321
url https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875
identifier_str_mv 10.34119/bjhrv7n1-321
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/66875/47669
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Health Review; Vol. 7 No. 1 (2024); 3958-3974
Brazilian Journal of Health Review; Vol. 7 Núm. 1 (2024); 3958-3974
Brazilian Journal of Health Review; v. 7 n. 1 (2024); 3958-3974
2595-6825
reponame:Brazilian Journal of Health Review
instname:Federação das Indústrias do Estado do Paraná (FIEP)
instacron:BJRH
instname_str Federação das Indústrias do Estado do Paraná (FIEP)
instacron_str BJRH
institution BJRH
reponame_str Brazilian Journal of Health Review
collection Brazilian Journal of Health Review
repository.name.fl_str_mv Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)
repository.mail.fl_str_mv || brazilianjhr@gmail.com
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