Investigation of vaccinia virus in bovines and buffaloes from the Marajó region, Brazil: standardization of a diagnostic test

• Autor(a) principal: de Melo, Taynah Cohen
• Data de Publicação: 2023
• Outros Autores: Siqueira, Jones Anderson Monteiro, Guerra, Sylvia de Fátima dos Santos, Moraes, Adriana Freitas, Casseb, Lívia Medeiros Neves, Farias, Luana da Silva Soares, Cruz, Ana Cecilia Ribeiro, Wanzeller, Ana Lucia Monteiro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Health Review
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/58694
• topic: Orthopoxvirus; Poxviridae; buffalopox viruses; cattle diseases; bison

The genus Orthopoxvirus (OPXV), belongs to the family Poxviridae and has 12 species, including Vaccinia virus (VACV) and Smallpox virus (VARV). Clinically, lesions in cattle are mostly seen on the teats and udders of lactating cows, which appear as bloody sores. The course of infection to complete healing takes two to four weeks. In humans, infections are currently occupational zoonoses. Little is still known about the ecoepidemiology of these viral agents, requiring further studies to seek alternatives for infection prevention and control. We sought to evaluate the epidemiology of VACV in asymptomatic buffaloes and cattle from farms on Marajó region, Brazil. Serum samples of 247 animals were tested collected in 2009. Viral DNA was extracted using commercial kits and detection was performed by the Polymerase Chain Reaction (PCR), targeting the growth factor gene of Vaccinia (VGF), followed by Nested PCR. Samples were undetectable to VACV by the methods used in the animals studied, requiring continuous epidemiological surveillance in the region, especially considering previous reports of outbreaks involving poxviruses in the country.