Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV

Detalhes bibliográficos
Autor(a) principal: Lima, Maria Fernanda Ferreira de
Data de Publicação: 2019
Outros Autores: França, Gislainy Isrraiany da Silva, Souza, Diogo Jorge Fragoso de, Cabral, Analucia Guedes Silveira, Filho, Clayton Anderson de Azevedo, Lima, Ellison Neves de, Sousa, Carlos Eduardo Miranda de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Health Review
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053
Resumo: This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs.
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spelling Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UVCaffeineDecaffeinated coffeeHPLC-UVValidationThis study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs.Brazilian Journals Publicações de Periódicos e Editora Ltda.2019-10-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/405310.34119/bjhr2n5-061Brazilian Journal of Health Review; Vol. 2 No. 5 (2019); 4600-4610Brazilian Journal of Health Review; v. 2 n. 5 (2019); 4600-46102595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053/3839Copyright (c) 2019 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessLima, Maria Fernanda Ferreira deFrança, Gislainy Isrraiany da SilvaSouza, Diogo Jorge Fragoso deCabral, Analucia Guedes SilveiraFilho, Clayton Anderson de AzevedoLima, Ellison Neves deSousa, Carlos Eduardo Miranda de2019-11-07T12:55:54Zoai:ojs2.ojs.brazilianjournals.com.br:article/4053Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2019-11-07T12:55:54Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false
dc.title.none.fl_str_mv Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
title Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
spellingShingle Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
Lima, Maria Fernanda Ferreira de
Caffeine
Decaffeinated coffee
HPLC-UV
Validation
title_short Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
title_full Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
title_fullStr Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
title_full_unstemmed Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
title_sort Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
author Lima, Maria Fernanda Ferreira de
author_facet Lima, Maria Fernanda Ferreira de
França, Gislainy Isrraiany da Silva
Souza, Diogo Jorge Fragoso de
Cabral, Analucia Guedes Silveira
Filho, Clayton Anderson de Azevedo
Lima, Ellison Neves de
Sousa, Carlos Eduardo Miranda de
author_role author
author2 França, Gislainy Isrraiany da Silva
Souza, Diogo Jorge Fragoso de
Cabral, Analucia Guedes Silveira
Filho, Clayton Anderson de Azevedo
Lima, Ellison Neves de
Sousa, Carlos Eduardo Miranda de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Maria Fernanda Ferreira de
França, Gislainy Isrraiany da Silva
Souza, Diogo Jorge Fragoso de
Cabral, Analucia Guedes Silveira
Filho, Clayton Anderson de Azevedo
Lima, Ellison Neves de
Sousa, Carlos Eduardo Miranda de
dc.subject.por.fl_str_mv Caffeine
Decaffeinated coffee
HPLC-UV
Validation
topic Caffeine
Decaffeinated coffee
HPLC-UV
Validation
description This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053
10.34119/bjhr2n5-061
url https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053
identifier_str_mv 10.34119/bjhr2n5-061
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053/3839
dc.rights.driver.fl_str_mv Copyright (c) 2019 Brazilian Journal of Health Review
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Brazilian Journal of Health Review
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Health Review; Vol. 2 No. 5 (2019); 4600-4610
Brazilian Journal of Health Review; v. 2 n. 5 (2019); 4600-4610
2595-6825
reponame:Brazilian Journal of Health Review
instname:Federação das Indústrias do Estado do Paraná (FIEP)
instacron:BJRH
instname_str Federação das Indústrias do Estado do Paraná (FIEP)
instacron_str BJRH
institution BJRH
reponame_str Brazilian Journal of Health Review
collection Brazilian Journal of Health Review
repository.name.fl_str_mv Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)
repository.mail.fl_str_mv || brazilianjhr@gmail.com
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