Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053 |
Resumo: | This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs. |
id |
BJRH-0_8fd42833be7925a3dd825f87de98643a |
---|---|
oai_identifier_str |
oai:ojs2.ojs.brazilianjournals.com.br:article/4053 |
network_acronym_str |
BJRH-0 |
network_name_str |
Brazilian Journal of Health Review |
repository_id_str |
|
spelling |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UVCaffeineDecaffeinated coffeeHPLC-UVValidationThis study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs.Brazilian Journals Publicações de Periódicos e Editora Ltda.2019-10-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/405310.34119/bjhr2n5-061Brazilian Journal of Health Review; Vol. 2 No. 5 (2019); 4600-4610Brazilian Journal of Health Review; v. 2 n. 5 (2019); 4600-46102595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053/3839Copyright (c) 2019 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessLima, Maria Fernanda Ferreira deFrança, Gislainy Isrraiany da SilvaSouza, Diogo Jorge Fragoso deCabral, Analucia Guedes SilveiraFilho, Clayton Anderson de AzevedoLima, Ellison Neves deSousa, Carlos Eduardo Miranda de2019-11-07T12:55:54Zoai:ojs2.ojs.brazilianjournals.com.br:article/4053Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2019-11-07T12:55:54Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
title |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
spellingShingle |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV Lima, Maria Fernanda Ferreira de Caffeine Decaffeinated coffee HPLC-UV Validation |
title_short |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
title_full |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
title_fullStr |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
title_full_unstemmed |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
title_sort |
Analytical validation of caffeine quantification method in decaffeinated coffee by HPLC-UV/ Validação analítica do método de quantificação de cafeína em café descafeinado por HPLC-UV |
author |
Lima, Maria Fernanda Ferreira de |
author_facet |
Lima, Maria Fernanda Ferreira de França, Gislainy Isrraiany da Silva Souza, Diogo Jorge Fragoso de Cabral, Analucia Guedes Silveira Filho, Clayton Anderson de Azevedo Lima, Ellison Neves de Sousa, Carlos Eduardo Miranda de |
author_role |
author |
author2 |
França, Gislainy Isrraiany da Silva Souza, Diogo Jorge Fragoso de Cabral, Analucia Guedes Silveira Filho, Clayton Anderson de Azevedo Lima, Ellison Neves de Sousa, Carlos Eduardo Miranda de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Maria Fernanda Ferreira de França, Gislainy Isrraiany da Silva Souza, Diogo Jorge Fragoso de Cabral, Analucia Guedes Silveira Filho, Clayton Anderson de Azevedo Lima, Ellison Neves de Sousa, Carlos Eduardo Miranda de |
dc.subject.por.fl_str_mv |
Caffeine Decaffeinated coffee HPLC-UV Validation |
topic |
Caffeine Decaffeinated coffee HPLC-UV Validation |
description |
This study aimed to determine the caffeine content and to verify that the contents of decaffeinated coffee are within the standards determined by regulatory agencies. This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.8 µg/mL) analytical method for the quantitation of Caffeine (CFF) in decaffeinated coffee. The method is based on High-performance Liquid chromatography-Ultraviolet (HPLC-UV) with a wavelength of 285nm. Sample preparation involved liquid-liquid extraction with chloroform. The chromatographic separation was achieved on a ACE C18 (150 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (20:80 v/v), in isocratic conditions. The assay was linear in the concentration range of 10 – 100 µg/mL. The mean recovery for CFF was 99.89%. Intra- and inter-day precision (R.S.D.) were < 1.5 % and <1.82 %, respectively and the accuracy (R.E.) was in the range ± 2.14 %.The results, verified by the validation, showed an adequate technique to be applied in the samples evaluated, in addition to a simple, fast execution methodology, with lower costs. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053 10.34119/bjhr2n5-061 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053 |
identifier_str_mv |
10.34119/bjhr2n5-061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4053/3839 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Journal of Health Review info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Journal of Health Review |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 2 No. 5 (2019); 4600-4610 Brazilian Journal of Health Review; v. 2 n. 5 (2019); 4600-4610 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
_version_ |
1797240049034592256 |