Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci

Detalhes bibliográficos
Autor(a) principal: Secchi,Carina
Data de Publicação: 2008
Outros Autores: Antunes,Ana Lúcia Souza, Perez,Leandro Reus Rodrigues, Cantarelli,Vlademir Vicente, d'Azevedo,Pedro Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012
Resumo: The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin gave a specificity of 88%. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.
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spelling Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococciCoagulase-negative StaphylococcuscefoxitinoxacillinmecA genesusceptibility diagnosticThe NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin gave a specificity of 88%. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.Brazilian Society of Infectious Diseases2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012Brazilian Journal of Infectious Diseases v.12 n.4 2008reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702008000400012info:eu-repo/semantics/openAccessSecchi,CarinaAntunes,Ana Lúcia SouzaPerez,Leandro Reus RodriguesCantarelli,Vlademir Vicented'Azevedo,Pedro Alveseng2008-11-13T00:00:00Zoai:scielo:S1413-86702008000400012Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2008-11-13T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
title Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
spellingShingle Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
Secchi,Carina
Coagulase-negative Staphylococcus
cefoxitin
oxacillin
mecA gene
susceptibility diagnostic
title_short Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
title_full Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
title_fullStr Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
title_full_unstemmed Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
title_sort Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
author Secchi,Carina
author_facet Secchi,Carina
Antunes,Ana Lúcia Souza
Perez,Leandro Reus Rodrigues
Cantarelli,Vlademir Vicente
d'Azevedo,Pedro Alves
author_role author
author2 Antunes,Ana Lúcia Souza
Perez,Leandro Reus Rodrigues
Cantarelli,Vlademir Vicente
d'Azevedo,Pedro Alves
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Secchi,Carina
Antunes,Ana Lúcia Souza
Perez,Leandro Reus Rodrigues
Cantarelli,Vlademir Vicente
d'Azevedo,Pedro Alves
dc.subject.por.fl_str_mv Coagulase-negative Staphylococcus
cefoxitin
oxacillin
mecA gene
susceptibility diagnostic
topic Coagulase-negative Staphylococcus
cefoxitin
oxacillin
mecA gene
susceptibility diagnostic
description The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin gave a specificity of 88%. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1413-86702008000400012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.12 n.4 2008
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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