Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004 |
Resumo: | INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes. |
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Brazilian Journal of Infectious Diseases |
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Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolatesStaphylococcusresistanceerm genesmacrolidesINTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes.Brazilian Society of Infectious Diseases2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004Brazilian Journal of Infectious Diseases v.14 n.6 2010reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702010000600004info:eu-repo/semantics/openAccessCoutinho,Vivian de Lima SpodePaiva,Rodrigo MinutoReiter,Keli Cristinede-Paris,FernandaBarth,Afonso LuisMachado,Alice Beatriz Mombach Pinheiroeng2011-02-14T00:00:00Zoai:scielo:S1413-86702010000600004Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2011-02-14T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
spellingShingle |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates Coutinho,Vivian de Lima Spode Staphylococcus resistance erm genes macrolides |
title_short |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_full |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_fullStr |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_full_unstemmed |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
title_sort |
Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates |
author |
Coutinho,Vivian de Lima Spode |
author_facet |
Coutinho,Vivian de Lima Spode Paiva,Rodrigo Minuto Reiter,Keli Cristine de-Paris,Fernanda Barth,Afonso Luis Machado,Alice Beatriz Mombach Pinheiro |
author_role |
author |
author2 |
Paiva,Rodrigo Minuto Reiter,Keli Cristine de-Paris,Fernanda Barth,Afonso Luis Machado,Alice Beatriz Mombach Pinheiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Coutinho,Vivian de Lima Spode Paiva,Rodrigo Minuto Reiter,Keli Cristine de-Paris,Fernanda Barth,Afonso Luis Machado,Alice Beatriz Mombach Pinheiro |
dc.subject.por.fl_str_mv |
Staphylococcus resistance erm genes macrolides |
topic |
Staphylococcus resistance erm genes macrolides |
description |
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by the disk diffusion method. Isolates with iMLS B phenotype were tested by double-disk induction method. All isolates were tested by a genotypic assay, PCR with specific primers. RESULTS: A total of 46.7% of staphylococci were positive for cMLS B; 3.3% for iMLS B and 3.3% for MS B. One or more erm genes were present in 50.1% of isolates. The gene ermA was detected in 49 isolates, ermC in 29 and ermB in 3. CONCLUSION: The prevalence of the ermA, ermB and ermC genes were 29.6%, 17.1% and 0.66% respectively, and constitutive resistance was the most frequent as compared to the other two phenotypes. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702010000600004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.14 n.6 2010 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209241508347904 |