Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions

Detalhes bibliográficos
Autor(a) principal: Sahaza,Jorge H.
Data de Publicação: 2020
Outros Autores: Rodríguez-Arellanez,Gabriela, Canteros,Cristina E., Reyes-Montes,María del Rocío, Taylor,Maria Lucia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044
Resumo: ABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America.
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spelling Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regionsLatin AmericaH. capsulatumYeastsThermotoleranceThermosensitivityABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America.Brazilian Society of Infectious Diseases2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044Brazilian Journal of Infectious Diseases v.24 n.1 2020reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2019.12.007info:eu-repo/semantics/openAccessSahaza,Jorge H.Rodríguez-Arellanez,GabrielaCanteros,Cristina E.Reyes-Montes,María del RocíoTaylor,Maria Luciaeng2020-04-02T00:00:00Zoai:scielo:S1413-86702020000100044Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2020-04-02T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
title Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
spellingShingle Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
Sahaza,Jorge H.
Latin America
H. capsulatum
Yeasts
Thermotolerance
Thermosensitivity
title_short Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
title_full Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
title_fullStr Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
title_full_unstemmed Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
title_sort Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
author Sahaza,Jorge H.
author_facet Sahaza,Jorge H.
Rodríguez-Arellanez,Gabriela
Canteros,Cristina E.
Reyes-Montes,María del Rocío
Taylor,Maria Lucia
author_role author
author2 Rodríguez-Arellanez,Gabriela
Canteros,Cristina E.
Reyes-Montes,María del Rocío
Taylor,Maria Lucia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sahaza,Jorge H.
Rodríguez-Arellanez,Gabriela
Canteros,Cristina E.
Reyes-Montes,María del Rocío
Taylor,Maria Lucia
dc.subject.por.fl_str_mv Latin America
H. capsulatum
Yeasts
Thermotolerance
Thermosensitivity
topic Latin America
H. capsulatum
Yeasts
Thermotolerance
Thermosensitivity
description ABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjid.2019.12.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.24 n.1 2020
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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