Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044 |
Resumo: | ABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America. |
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Brazilian Journal of Infectious Diseases |
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Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regionsLatin AmericaH. capsulatumYeastsThermotoleranceThermosensitivityABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America.Brazilian Society of Infectious Diseases2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044Brazilian Journal of Infectious Diseases v.24 n.1 2020reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2019.12.007info:eu-repo/semantics/openAccessSahaza,Jorge H.Rodríguez-Arellanez,GabrielaCanteros,Cristina E.Reyes-Montes,María del RocíoTaylor,Maria Luciaeng2020-04-02T00:00:00Zoai:scielo:S1413-86702020000100044Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2020-04-02T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
spellingShingle |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions Sahaza,Jorge H. Latin America H. capsulatum Yeasts Thermotolerance Thermosensitivity |
title_short |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_full |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_fullStr |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_full_unstemmed |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
title_sort |
Thermotolerance of Histoplasma capsulatum at 40 °C predominates among clinical isolates from different Latin American regions |
author |
Sahaza,Jorge H. |
author_facet |
Sahaza,Jorge H. Rodríguez-Arellanez,Gabriela Canteros,Cristina E. Reyes-Montes,María del Rocío Taylor,Maria Lucia |
author_role |
author |
author2 |
Rodríguez-Arellanez,Gabriela Canteros,Cristina E. Reyes-Montes,María del Rocío Taylor,Maria Lucia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sahaza,Jorge H. Rodríguez-Arellanez,Gabriela Canteros,Cristina E. Reyes-Montes,María del Rocío Taylor,Maria Lucia |
dc.subject.por.fl_str_mv |
Latin America H. capsulatum Yeasts Thermotolerance Thermosensitivity |
topic |
Latin America H. capsulatum Yeasts Thermotolerance Thermosensitivity |
description |
ABSTRACT The yeast phase of 22 Histoplasma capsulatum clinical isolates from Mexico, Argentina, Colombia, and Guatemala and three reference strains, one from Panama and two from the United States of America (USA), were screened for thermosensitivity characteristics using different analyses. Growth curves at 0, 3, 6, 12, 24, and 30 h of incubation at 37 and 40 °C, the growth inhibition percentage at 40 °C, and the doubling time at 37 and 40 °C were determined for all yeasts studied. Most of the isolates examined exhibited thermotolerant phenotypes at 40 °C, whereas a thermosensitive phenotype at 40 °C was only detected in the Downs reference strain from the USA. Growth inhibition values lower than 33.8% supported the predominance of the thermotolerant phenotype at 40 °C. The doubling time means found for the different isolates were 5.14 h ± 1.47 h at 37 °C and 5.55 h ± 1.87 h at 40 °C. This is the first report to underscore the predominance of thermotolerant and delayed doubling time phenotypes in H. capsulatum clinical isolates from different regions of Latin America. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100044 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2019.12.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.24 n.1 2020 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209244732719104 |