Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008 |
Resumo: | Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment. |
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Brazilian Journal of Infectious Diseases |
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Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samplesHuman rabiesneck-skindiagnosisRT-PCRDNA sequencingRapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment.Brazilian Society of Infectious Diseases2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008Brazilian Journal of Infectious Diseases v.10 n.5 2006reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702006000500008info:eu-repo/semantics/openAccessMacedo,Carla IsabelCarnieli Jr,PedroBrandão,Paulo EduardoRosa,Elizabeth S. Travassos daOliveira,Rafael de NovaesCastilho,Juliana GaleraMedeiros,RitaMachado,Rosangela RochaOliveira,Rosely Cerqueira deCarrieri,Maria LuizaKotait,Ivaneteeng2007-01-31T00:00:00Zoai:scielo:S1413-86702006000500008Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2007-01-31T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
title |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
spellingShingle |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples Macedo,Carla Isabel Human rabies neck-skin diagnosis RT-PCR DNA sequencing |
title_short |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
title_full |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
title_fullStr |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
title_full_unstemmed |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
title_sort |
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples |
author |
Macedo,Carla Isabel |
author_facet |
Macedo,Carla Isabel Carnieli Jr,Pedro Brandão,Paulo Eduardo Rosa,Elizabeth S. Travassos da Oliveira,Rafael de Novaes Castilho,Juliana Galera Medeiros,Rita Machado,Rosangela Rocha Oliveira,Rosely Cerqueira de Carrieri,Maria Luiza Kotait,Ivanete |
author_role |
author |
author2 |
Carnieli Jr,Pedro Brandão,Paulo Eduardo Rosa,Elizabeth S. Travassos da Oliveira,Rafael de Novaes Castilho,Juliana Galera Medeiros,Rita Machado,Rosangela Rocha Oliveira,Rosely Cerqueira de Carrieri,Maria Luiza Kotait,Ivanete |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Macedo,Carla Isabel Carnieli Jr,Pedro Brandão,Paulo Eduardo Rosa,Elizabeth S. Travassos da Oliveira,Rafael de Novaes Castilho,Juliana Galera Medeiros,Rita Machado,Rosangela Rocha Oliveira,Rosely Cerqueira de Carrieri,Maria Luiza Kotait,Ivanete |
dc.subject.por.fl_str_mv |
Human rabies neck-skin diagnosis RT-PCR DNA sequencing |
topic |
Human rabies neck-skin diagnosis RT-PCR DNA sequencing |
description |
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702006000500008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.10 n.5 2006 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209239470964736 |